INTERLEUKIN-10 REGULATES PD-1-B7-H1-MEDIATED CYTOTOXIC ACTIVITY OF DENDRITIC CELLS
In this investigation the phenotypic and functional properties of healthy donor and patient with pulmonary tuberculosis (PT) dendritic cells (DCs) were characterized. We also studied the influence of IL-10 on the phenotype and apoptosis-inducing activity of healthy donor DCs. 60 patients with pulmon...
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| Format: | Article |
| Language: | Russian |
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Scientific Сentre for Family Health and Human Reproduction Problems
2012-05-01
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| Series: | Acta Biomedica Scientifica |
| Subjects: | |
| Online Access: | https://www.actabiomedica.ru/jour/article/view/911 |
| _version_ | 1797237676993150976 |
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| author | L. V. Sakhno M. A. Tikhonova O. Yu. Leplina A. A. Ostanin E. R. Chernykh |
| author_facet | L. V. Sakhno M. A. Tikhonova O. Yu. Leplina A. A. Ostanin E. R. Chernykh |
| author_sort | L. V. Sakhno |
| collection | DOAJ |
| description | In this investigation the phenotypic and functional properties of healthy donor and patient with pulmonary tuberculosis (PT) dendritic cells (DCs) were characterized. We also studied the influence of IL-10 on the phenotype and apoptosis-inducing activity of healthy donor DCs. 60 patients with pulmonary tuberculosis with different proliferative response to antigens of M. tuberculosis (purified protein derivative, PPD) and 40 healthy donors were enrolled in this study. It was revealed that DCs, generated in vitro from PT patient's blood monocytes with GM-CSF+IFN-α, were characterized by increased B7-H1 expression, up-production of IL-10 and reducing of allostimulatory activity in mixed lymphocyte culture (MLC). The endogenous IL-10 production by DCs was correlated with expression of B7-H1 in the general group of persons. It was revealed that addition of IL-10 to semi-mature DCs of healthy donor results in increasing of B7-H1 expression, diminishing of allostimulatory activity and enlargement of pro-apoptogenic activity of DCs. |
| first_indexed | 2024-03-08T12:22:56Z |
| format | Article |
| id | doaj.art-3e9f8c7b965e4a8ba2049c947a7bcd87 |
| institution | Directory Open Access Journal |
| issn | 2541-9420 2587-9596 |
| language | Russian |
| last_indexed | 2024-04-24T17:23:33Z |
| publishDate | 2012-05-01 |
| publisher | Scientific Сentre for Family Health and Human Reproduction Problems |
| record_format | Article |
| series | Acta Biomedica Scientifica |
| spelling | doaj.art-3e9f8c7b965e4a8ba2049c947a7bcd872024-03-28T07:49:04ZrusScientific Сentre for Family Health and Human Reproduction ProblemsActa Biomedica Scientifica2541-94202587-95962012-05-0103(2)170174857INTERLEUKIN-10 REGULATES PD-1-B7-H1-MEDIATED CYTOTOXIC ACTIVITY OF DENDRITIC CELLSL. V. Sakhno0M. A. Tikhonova1O. Yu. Leplina2A. A. Ostanin3E. R. Chernykh4Research Institute of Clinical Immunology SB RAMSResearch Institute of Clinical Immunology SB RAMSResearch Institute of Clinical Immunology SB RAMSResearch Institute of Clinical Immunology SB RAMSResearch Institute of Clinical Immunology SB RAMSIn this investigation the phenotypic and functional properties of healthy donor and patient with pulmonary tuberculosis (PT) dendritic cells (DCs) were characterized. We also studied the influence of IL-10 on the phenotype and apoptosis-inducing activity of healthy donor DCs. 60 patients with pulmonary tuberculosis with different proliferative response to antigens of M. tuberculosis (purified protein derivative, PPD) and 40 healthy donors were enrolled in this study. It was revealed that DCs, generated in vitro from PT patient's blood monocytes with GM-CSF+IFN-α, were characterized by increased B7-H1 expression, up-production of IL-10 and reducing of allostimulatory activity in mixed lymphocyte culture (MLC). The endogenous IL-10 production by DCs was correlated with expression of B7-H1 in the general group of persons. It was revealed that addition of IL-10 to semi-mature DCs of healthy donor results in increasing of B7-H1 expression, diminishing of allostimulatory activity and enlargement of pro-apoptogenic activity of DCs.https://www.actabiomedica.ru/jour/article/view/911b7-h1il-10b7-h1dendritic cellsapoptosisil-10 |
| spellingShingle | L. V. Sakhno M. A. Tikhonova O. Yu. Leplina A. A. Ostanin E. R. Chernykh INTERLEUKIN-10 REGULATES PD-1-B7-H1-MEDIATED CYTOTOXIC ACTIVITY OF DENDRITIC CELLS Acta Biomedica Scientifica b7-h1 il-10 b7-h1 dendritic cells apoptosis il-10 |
| title | INTERLEUKIN-10 REGULATES PD-1-B7-H1-MEDIATED CYTOTOXIC ACTIVITY OF DENDRITIC CELLS |
| title_full | INTERLEUKIN-10 REGULATES PD-1-B7-H1-MEDIATED CYTOTOXIC ACTIVITY OF DENDRITIC CELLS |
| title_fullStr | INTERLEUKIN-10 REGULATES PD-1-B7-H1-MEDIATED CYTOTOXIC ACTIVITY OF DENDRITIC CELLS |
| title_full_unstemmed | INTERLEUKIN-10 REGULATES PD-1-B7-H1-MEDIATED CYTOTOXIC ACTIVITY OF DENDRITIC CELLS |
| title_short | INTERLEUKIN-10 REGULATES PD-1-B7-H1-MEDIATED CYTOTOXIC ACTIVITY OF DENDRITIC CELLS |
| title_sort | interleukin 10 regulates pd 1 b7 h1 mediated cytotoxic activity of dendritic cells |
| topic | b7-h1 il-10 b7-h1 dendritic cells apoptosis il-10 |
| url | https://www.actabiomedica.ru/jour/article/view/911 |
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