Comparison of separation methods for tissue‐derived extracellular vesicles in the liver, heart, and skeletal muscle
Extracellular vesicles (EVs), which are nanosized vesicles released by cells as intracellular messengers, have high potential as biomarkers. EVs are usually collected from in vitro sources, such as cell culture media or biofluids, and not from tissues. Techniques enabling direct collection of EVs fr...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2021-02-01
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| Series: | FEBS Open Bio |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/2211-5463.13075 |
| _version_ | 1827010825062187008 |
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| author | Adam Matejovič Shohei Wakao Masaaki Kitada Yoshihiro Kushida Mari Dezawa |
| author_facet | Adam Matejovič Shohei Wakao Masaaki Kitada Yoshihiro Kushida Mari Dezawa |
| author_sort | Adam Matejovič |
| collection | DOAJ |
| description | Extracellular vesicles (EVs), which are nanosized vesicles released by cells as intracellular messengers, have high potential as biomarkers. EVs are usually collected from in vitro sources, such as cell culture media or biofluids, and not from tissues. Techniques enabling direct collection of EVs from tissues will extend the applications of EVs. We compared methods for separating EVs from solid liver, heart, and skeletal muscle. Compared with a precipitation method, an ultracentrifugation‐based method for collection of EVs from solid tissues yielded a higher proportion of EVs positive for EV‐related markers, with minimum levels of intracellular organelle‐related markers. Some tissue‐specific modifications, such as a sucrose cushion step, may improve the yield and purity of the collected EVs. |
| first_indexed | 2024-04-13T06:37:20Z |
| format | Article |
| id | doaj.art-3eaf873549ef44f0a612fe264c4dd854 |
| institution | Directory Open Access Journal |
| issn | 2211-5463 |
| language | English |
| last_indexed | 2025-02-18T13:11:59Z |
| publishDate | 2021-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj.art-3eaf873549ef44f0a612fe264c4dd8542024-11-01T15:30:59ZengWileyFEBS Open Bio2211-54632021-02-0111248249310.1002/2211-5463.13075Comparison of separation methods for tissue‐derived extracellular vesicles in the liver, heart, and skeletal muscleAdam Matejovič0Shohei Wakao1Masaaki Kitada2Yoshihiro Kushida3Mari Dezawa4Department of Stem Cell Biology and Histology Tohoku University Graduate School of Medicine Sendai JapanDepartment of Stem Cell Biology and Histology Tohoku University Graduate School of Medicine Sendai JapanDepartment of Stem Cell Biology and Histology Tohoku University Graduate School of Medicine Sendai JapanDepartment of Stem Cell Biology and Histology Tohoku University Graduate School of Medicine Sendai JapanDepartment of Stem Cell Biology and Histology Tohoku University Graduate School of Medicine Sendai JapanExtracellular vesicles (EVs), which are nanosized vesicles released by cells as intracellular messengers, have high potential as biomarkers. EVs are usually collected from in vitro sources, such as cell culture media or biofluids, and not from tissues. Techniques enabling direct collection of EVs from tissues will extend the applications of EVs. We compared methods for separating EVs from solid liver, heart, and skeletal muscle. Compared with a precipitation method, an ultracentrifugation‐based method for collection of EVs from solid tissues yielded a higher proportion of EVs positive for EV‐related markers, with minimum levels of intracellular organelle‐related markers. Some tissue‐specific modifications, such as a sucrose cushion step, may improve the yield and purity of the collected EVs.https://doi.org/10.1002/2211-5463.13075exosomeextracellular vesiclesheartlivermuscletissue |
| spellingShingle | Adam Matejovič Shohei Wakao Masaaki Kitada Yoshihiro Kushida Mari Dezawa Comparison of separation methods for tissue‐derived extracellular vesicles in the liver, heart, and skeletal muscle FEBS Open Bio exosome extracellular vesicles heart liver muscle tissue |
| title | Comparison of separation methods for tissue‐derived extracellular vesicles in the liver, heart, and skeletal muscle |
| title_full | Comparison of separation methods for tissue‐derived extracellular vesicles in the liver, heart, and skeletal muscle |
| title_fullStr | Comparison of separation methods for tissue‐derived extracellular vesicles in the liver, heart, and skeletal muscle |
| title_full_unstemmed | Comparison of separation methods for tissue‐derived extracellular vesicles in the liver, heart, and skeletal muscle |
| title_short | Comparison of separation methods for tissue‐derived extracellular vesicles in the liver, heart, and skeletal muscle |
| title_sort | comparison of separation methods for tissue derived extracellular vesicles in the liver heart and skeletal muscle |
| topic | exosome extracellular vesicles heart liver muscle tissue |
| url | https://doi.org/10.1002/2211-5463.13075 |
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