Isolation and identification of yeasts in milk samples from cows' mammary glands

The purpose of this study was to isolate fungi from the milk of cow udder quarters with clinical mastitis. The samples were delivered in Veterinary laboratory in Križevci during a routine mastitis diagnostics. Milk samples were cultured on Columbia agar (Merck, KgaA, Darmstadt, Germany) with 5 % ovi...

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Main Authors: Vesna Jaki, Darko Majnarić, Jadranka Jurmanović, Željka Bažulić
Format: Article
Language:English
Published: Croatian Dairy Union 2007-06-01
Series:Mljekarstvo
Subjects:
Online Access:http://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=20689
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author Vesna Jaki
Darko Majnarić
Jadranka Jurmanović
Željka Bažulić
author_facet Vesna Jaki
Darko Majnarić
Jadranka Jurmanović
Željka Bažulić
author_sort Vesna Jaki
collection DOAJ
description The purpose of this study was to isolate fungi from the milk of cow udder quarters with clinical mastitis. The samples were delivered in Veterinary laboratory in Križevci during a routine mastitis diagnostics. Milk samples were cultured on Columbia agar (Merck, KgaA, Darmstadt, Germany) with 5 % ovine blood, Sabouraud 4 % maltose agar (Merck, KgaA, Darmstadt, Germany) and Rice extract agar (Merck, KgaA, Darmstadt, Germany). The final diagnosis was established regarding to the results of the API 20 C AUX systems (bioMerieux, Lyon, France). All of the fungal isolates were yeasts, genera Candida spp. (76.2 %) and Trichosporon spp. (23.8 %). The most prevalent species were: C. quilliermondi (21.4 %), C. krusei/inconspicua (11.9 %) and Trichosporon mucoides (14.3 %).
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spelling doaj.art-3eb349a42ab24abe8d887dd7cd3e583a2022-12-22T01:36:37ZengCroatian Dairy UnionMljekarstvo0026-704X1846-40252007-06-01572119125Isolation and identification of yeasts in milk samples from cows' mammary glandsVesna JakiDarko MajnarićJadranka JurmanovićŽeljka BažulićThe purpose of this study was to isolate fungi from the milk of cow udder quarters with clinical mastitis. The samples were delivered in Veterinary laboratory in Križevci during a routine mastitis diagnostics. Milk samples were cultured on Columbia agar (Merck, KgaA, Darmstadt, Germany) with 5 % ovine blood, Sabouraud 4 % maltose agar (Merck, KgaA, Darmstadt, Germany) and Rice extract agar (Merck, KgaA, Darmstadt, Germany). The final diagnosis was established regarding to the results of the API 20 C AUX systems (bioMerieux, Lyon, France). All of the fungal isolates were yeasts, genera Candida spp. (76.2 %) and Trichosporon spp. (23.8 %). The most prevalent species were: C. quilliermondi (21.4 %), C. krusei/inconspicua (11.9 %) and Trichosporon mucoides (14.3 %).http://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=20689yeastscow's milkmastitis
spellingShingle Vesna Jaki
Darko Majnarić
Jadranka Jurmanović
Željka Bažulić
Isolation and identification of yeasts in milk samples from cows' mammary glands
Mljekarstvo
yeasts
cow's milk
mastitis
title Isolation and identification of yeasts in milk samples from cows' mammary glands
title_full Isolation and identification of yeasts in milk samples from cows' mammary glands
title_fullStr Isolation and identification of yeasts in milk samples from cows' mammary glands
title_full_unstemmed Isolation and identification of yeasts in milk samples from cows' mammary glands
title_short Isolation and identification of yeasts in milk samples from cows' mammary glands
title_sort isolation and identification of yeasts in milk samples from cows mammary glands
topic yeasts
cow's milk
mastitis
url http://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=20689
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AT jadrankajurmanovic isolationandidentificationofyeastsinmilksamplesfromcowsmammaryglands
AT zeljkabazulic isolationandidentificationofyeastsinmilksamplesfromcowsmammaryglands