Metagenomic insights into the wastewater resistome before and after purification at large‑scale wastewater treatment plants in the Moscow city
Abstract Wastewater treatment plants (WWTPs) are considered to be hotspots for the spread of antibiotic resistance genes (ARGs). We performed a metagenomic analysis of the raw wastewater, activated sludge and treated wastewater from two large WWTPs responsible for the treatment of urban wastewater i...
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| Format: | Article |
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Nature Portfolio
2024-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-56870-0 |
| _version_ | 1797259342301364224 |
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| author | Shahjahon Begmatov Alexey V. Beletsky Alexander G. Dorofeev Nikolai V. Pimenov Andrey V. Mardanov Nikolai V. Ravin |
| author_facet | Shahjahon Begmatov Alexey V. Beletsky Alexander G. Dorofeev Nikolai V. Pimenov Andrey V. Mardanov Nikolai V. Ravin |
| author_sort | Shahjahon Begmatov |
| collection | DOAJ |
| description | Abstract Wastewater treatment plants (WWTPs) are considered to be hotspots for the spread of antibiotic resistance genes (ARGs). We performed a metagenomic analysis of the raw wastewater, activated sludge and treated wastewater from two large WWTPs responsible for the treatment of urban wastewater in Moscow, Russia. In untreated wastewater, several hundred ARGs that could confer resistance to most commonly used classes of antibiotics were found. WWTPs employed a nitrification/denitrification or an anaerobic/anoxic/oxic process and enabled efficient removal of organic matter, nitrogen and phosphorus, as well as fecal microbiota. The resistome constituted about 0.05% of the whole metagenome, and after water treatment its share decreased by 3–4 times. The resistomes were dominated by ARGs encoding resistance to beta-lactams, macrolides, aminoglycosides, tetracyclines, quaternary ammonium compounds, and sulfonamides. ARGs for macrolides and tetracyclines were removed more efficiently than beta-lactamases, especially ampC, the most abundant ARG in the treated effluent. The removal efficiency of particular ARGs was impacted by the treatment technology. Metagenome-assembled genomes of multidrug-resistant strains were assembled both for the influent and the treated effluent. Ccomparison of resistomes from WWTPs in Moscow and around the world suggested that the abundance and content of ARGs depend on social, economic, medical, and environmental factors. |
| first_indexed | 2024-04-24T23:07:54Z |
| format | Article |
| id | doaj.art-3eb6d1f863e14dbeaece9e7fcf5c6a4d |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-24T23:07:54Z |
| publishDate | 2024-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-3eb6d1f863e14dbeaece9e7fcf5c6a4d2024-03-17T12:21:54ZengNature PortfolioScientific Reports2045-23222024-03-0114111210.1038/s41598-024-56870-0Metagenomic insights into the wastewater resistome before and after purification at large‑scale wastewater treatment plants in the Moscow cityShahjahon Begmatov0Alexey V. Beletsky1Alexander G. Dorofeev2Nikolai V. Pimenov3Andrey V. Mardanov4Nikolai V. Ravin5Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of SciencesInstitute of Bioengineering, Research Center of Biotechnology of the Russian Academy of SciencesWinogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of SciencesWinogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of SciencesInstitute of Bioengineering, Research Center of Biotechnology of the Russian Academy of SciencesInstitute of Bioengineering, Research Center of Biotechnology of the Russian Academy of SciencesAbstract Wastewater treatment plants (WWTPs) are considered to be hotspots for the spread of antibiotic resistance genes (ARGs). We performed a metagenomic analysis of the raw wastewater, activated sludge and treated wastewater from two large WWTPs responsible for the treatment of urban wastewater in Moscow, Russia. In untreated wastewater, several hundred ARGs that could confer resistance to most commonly used classes of antibiotics were found. WWTPs employed a nitrification/denitrification or an anaerobic/anoxic/oxic process and enabled efficient removal of organic matter, nitrogen and phosphorus, as well as fecal microbiota. The resistome constituted about 0.05% of the whole metagenome, and after water treatment its share decreased by 3–4 times. The resistomes were dominated by ARGs encoding resistance to beta-lactams, macrolides, aminoglycosides, tetracyclines, quaternary ammonium compounds, and sulfonamides. ARGs for macrolides and tetracyclines were removed more efficiently than beta-lactamases, especially ampC, the most abundant ARG in the treated effluent. The removal efficiency of particular ARGs was impacted by the treatment technology. Metagenome-assembled genomes of multidrug-resistant strains were assembled both for the influent and the treated effluent. Ccomparison of resistomes from WWTPs in Moscow and around the world suggested that the abundance and content of ARGs depend on social, economic, medical, and environmental factors.https://doi.org/10.1038/s41598-024-56870-0 |
| spellingShingle | Shahjahon Begmatov Alexey V. Beletsky Alexander G. Dorofeev Nikolai V. Pimenov Andrey V. Mardanov Nikolai V. Ravin Metagenomic insights into the wastewater resistome before and after purification at large‑scale wastewater treatment plants in the Moscow city Scientific Reports |
| title | Metagenomic insights into the wastewater resistome before and after purification at large‑scale wastewater treatment plants in the Moscow city |
| title_full | Metagenomic insights into the wastewater resistome before and after purification at large‑scale wastewater treatment plants in the Moscow city |
| title_fullStr | Metagenomic insights into the wastewater resistome before and after purification at large‑scale wastewater treatment plants in the Moscow city |
| title_full_unstemmed | Metagenomic insights into the wastewater resistome before and after purification at large‑scale wastewater treatment plants in the Moscow city |
| title_short | Metagenomic insights into the wastewater resistome before and after purification at large‑scale wastewater treatment plants in the Moscow city |
| title_sort | metagenomic insights into the wastewater resistome before and after purification at large scale wastewater treatment plants in the moscow city |
| url | https://doi.org/10.1038/s41598-024-56870-0 |
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