Comparative Analysis of Substance Content, Antioxidant and α-Glucosidase Inhibitory Activity of Each Component of Yaan Tibetan Tea after Water Extraction and Alcohol Precipitation

In order to study the distribution of components (aqueous extract, alcohol precipitation and supernate) in the water extract of Tibetan tea after alcohol precipitation, the basic components, total polyphenols, total flavonoids, and tea pigments were comprehensively analyzed and compared. The content...

Full description

Bibliographic Details
Main Authors: Chen XIA, Kebin LUO, Zhuoya XIANG, Junlin DENG, Jian CHEN, Yongqing ZHU, Liugang SHI
Format: Article
Language:zho
Published: The editorial department of Science and Technology of Food Industry 2022-11-01
Series:Shipin gongye ke-ji
Subjects:
Online Access:http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022010011
_version_ 1798017297555652608
author Chen XIA
Kebin LUO
Zhuoya XIANG
Junlin DENG
Jian CHEN
Yongqing ZHU
Liugang SHI
author_facet Chen XIA
Kebin LUO
Zhuoya XIANG
Junlin DENG
Jian CHEN
Yongqing ZHU
Liugang SHI
author_sort Chen XIA
collection DOAJ
description In order to study the distribution of components (aqueous extract, alcohol precipitation and supernate) in the water extract of Tibetan tea after alcohol precipitation, the basic components, total polyphenols, total flavonoids, and tea pigments were comprehensively analyzed and compared. The contents of polyphenols were detected by high performance liquid chromatography, and the differences of DPPH· and ABTS+· scavenging abilities and inhibitory effects on α-glucosidase digestive enzyme were investigated. Results indicated that the contents of total sugar (17.73 g/100 g), total polysaccharide (16.93 g/100 g), amino acid (5.89 g/100 g) and the abrownin (49.18 g/100 g) in the alcohol precipitation were significantly higher than those in the supernatant. However, the contents of total polyphenols (195.93 mg/g), total flavonoids (61.61 mg/g) and main polyphenols (GA, GC, EGC, C, CA, EC, EGCG, GCG, ECG and CG) in the supernatant were significantly higher than those in the alcohol precipitation. The results of antioxidant activity and digestive enzyme inhibition activity in vitro showed that the DPPH· and ABTS+· scavenging ability of alcohol precipitate was lower than that of supernatant, while had higher inhibition ability of α-glucosidase. The supernatant of Tibetan tea after alcohol precipitation had strong antioxidant activity, while the alcohol precipitation had strong digestive enzymes (α-glucosidase) inhibition activity.
first_indexed 2024-04-11T16:04:53Z
format Article
id doaj.art-3eb6ee7d6a014922be36bda96744ce0d
institution Directory Open Access Journal
issn 1002-0306
language zho
last_indexed 2024-04-11T16:04:53Z
publishDate 2022-11-01
publisher The editorial department of Science and Technology of Food Industry
record_format Article
series Shipin gongye ke-ji
spelling doaj.art-3eb6ee7d6a014922be36bda96744ce0d2022-12-22T04:14:52ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062022-11-014321697410.13386/j.issn1002-0306.20220100112022010011-21Comparative Analysis of Substance Content, Antioxidant and α-Glucosidase Inhibitory Activity of Each Component of Yaan Tibetan Tea after Water Extraction and Alcohol PrecipitationChen XIA0Kebin LUO1Zhuoya XIANG2Junlin DENG3Jian CHEN4Yongqing ZHU5Liugang SHI6Institute of Agro-Products Processing Science and Technology, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaInstitute of Agro-Products Processing Science and Technology, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaInstitute of Agro-Products Processing Science and Technology, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaInstitute of Agro-Products Processing Science and Technology, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaInstitute of Agro-Products Processing Science and Technology, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaInstitute of Agro-Products Processing Science and Technology, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaYaan Golden Flower Tibetan Tea Tea Industry Co., Ltd., Yaan 625100, ChinaIn order to study the distribution of components (aqueous extract, alcohol precipitation and supernate) in the water extract of Tibetan tea after alcohol precipitation, the basic components, total polyphenols, total flavonoids, and tea pigments were comprehensively analyzed and compared. The contents of polyphenols were detected by high performance liquid chromatography, and the differences of DPPH· and ABTS+· scavenging abilities and inhibitory effects on α-glucosidase digestive enzyme were investigated. Results indicated that the contents of total sugar (17.73 g/100 g), total polysaccharide (16.93 g/100 g), amino acid (5.89 g/100 g) and the abrownin (49.18 g/100 g) in the alcohol precipitation were significantly higher than those in the supernatant. However, the contents of total polyphenols (195.93 mg/g), total flavonoids (61.61 mg/g) and main polyphenols (GA, GC, EGC, C, CA, EC, EGCG, GCG, ECG and CG) in the supernatant were significantly higher than those in the alcohol precipitation. The results of antioxidant activity and digestive enzyme inhibition activity in vitro showed that the DPPH· and ABTS+· scavenging ability of alcohol precipitate was lower than that of supernatant, while had higher inhibition ability of α-glucosidase. The supernatant of Tibetan tea after alcohol precipitation had strong antioxidant activity, while the alcohol precipitation had strong digestive enzymes (α-glucosidase) inhibition activity.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022010011yaan tibetan tea extractalcohol precipitationpolyphenolantioxidant activityenzymes inhibition activity
spellingShingle Chen XIA
Kebin LUO
Zhuoya XIANG
Junlin DENG
Jian CHEN
Yongqing ZHU
Liugang SHI
Comparative Analysis of Substance Content, Antioxidant and α-Glucosidase Inhibitory Activity of Each Component of Yaan Tibetan Tea after Water Extraction and Alcohol Precipitation
Shipin gongye ke-ji
yaan tibetan tea extract
alcohol precipitation
polyphenol
antioxidant activity
enzymes inhibition activity
title Comparative Analysis of Substance Content, Antioxidant and α-Glucosidase Inhibitory Activity of Each Component of Yaan Tibetan Tea after Water Extraction and Alcohol Precipitation
title_full Comparative Analysis of Substance Content, Antioxidant and α-Glucosidase Inhibitory Activity of Each Component of Yaan Tibetan Tea after Water Extraction and Alcohol Precipitation
title_fullStr Comparative Analysis of Substance Content, Antioxidant and α-Glucosidase Inhibitory Activity of Each Component of Yaan Tibetan Tea after Water Extraction and Alcohol Precipitation
title_full_unstemmed Comparative Analysis of Substance Content, Antioxidant and α-Glucosidase Inhibitory Activity of Each Component of Yaan Tibetan Tea after Water Extraction and Alcohol Precipitation
title_short Comparative Analysis of Substance Content, Antioxidant and α-Glucosidase Inhibitory Activity of Each Component of Yaan Tibetan Tea after Water Extraction and Alcohol Precipitation
title_sort comparative analysis of substance content antioxidant and α glucosidase inhibitory activity of each component of yaan tibetan tea after water extraction and alcohol precipitation
topic yaan tibetan tea extract
alcohol precipitation
polyphenol
antioxidant activity
enzymes inhibition activity
url http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022010011
work_keys_str_mv AT chenxia comparativeanalysisofsubstancecontentantioxidantandaglucosidaseinhibitoryactivityofeachcomponentofyaantibetanteaafterwaterextractionandalcoholprecipitation
AT kebinluo comparativeanalysisofsubstancecontentantioxidantandaglucosidaseinhibitoryactivityofeachcomponentofyaantibetanteaafterwaterextractionandalcoholprecipitation
AT zhuoyaxiang comparativeanalysisofsubstancecontentantioxidantandaglucosidaseinhibitoryactivityofeachcomponentofyaantibetanteaafterwaterextractionandalcoholprecipitation
AT junlindeng comparativeanalysisofsubstancecontentantioxidantandaglucosidaseinhibitoryactivityofeachcomponentofyaantibetanteaafterwaterextractionandalcoholprecipitation
AT jianchen comparativeanalysisofsubstancecontentantioxidantandaglucosidaseinhibitoryactivityofeachcomponentofyaantibetanteaafterwaterextractionandalcoholprecipitation
AT yongqingzhu comparativeanalysisofsubstancecontentantioxidantandaglucosidaseinhibitoryactivityofeachcomponentofyaantibetanteaafterwaterextractionandalcoholprecipitation
AT liugangshi comparativeanalysisofsubstancecontentantioxidantandaglucosidaseinhibitoryactivityofeachcomponentofyaantibetanteaafterwaterextractionandalcoholprecipitation