Different dose-dependent effects of ebselen in sciatic nerve ischemia-reperfusion injury in rats
Ebselen is an organoselenium compound which has strong antioxidant and anti-inflammatory effects. We investigated the neuroprotective role of ebselen pretreatment in rats with experimental sciatic nerve ischemia-reperfusion (I/R) injury. Adult male Sprague Dawley rats were divided into four groups...
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| Format: | Article |
| Language: | English |
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Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina
2015-08-01
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| Series: | Biomolecules & Biomedicine |
| Subjects: | |
| Online Access: | https://www.bjbms.org/ojs/index.php/bjbms/article/view/521 |
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| author | Filiz Ozyigit Aysegul Kucuk Sezer Akcer Murat Tosun Emel Fatma Kocak Cengiz Kocak Ahmet Kocak Hasan Metineren Osman Genc |
| author_facet | Filiz Ozyigit Aysegul Kucuk Sezer Akcer Murat Tosun Emel Fatma Kocak Cengiz Kocak Ahmet Kocak Hasan Metineren Osman Genc |
| author_sort | Filiz Ozyigit |
| collection | DOAJ |
| description |
Ebselen is an organoselenium compound which has strong antioxidant and anti-inflammatory effects. We investigated the neuroprotective role of ebselen pretreatment in rats with experimental sciatic nerve ischemia-reperfusion (I/R) injury. Adult male Sprague Dawley rats were divided into four groups (N = 7 in each group). Before sciatic nerve I/R was induced, ebselen was injected intraperitoneally at doses of 15 and 30 mg/kg. After a 2 h ischemia and a 3 h reperfusion period, sciatic nerve tissues were excised. Tissue levels of malondialdehyde (MDA) and nitric oxide (NO), and activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) were measured. Sciatic nerve tissues were also examined histopathologically. The 15 mg/kg dose of ebselen reduced sciatic nerve damage and apoptosis (P < 0.01), levels of MDA, NO, and inducible nitric oxide synthase (iNOS) positive cells (P < 0.01, P < 0.05, respectively), and increased SOD, GPx, and CAT activities (P < 0.001, P < 0.01, P < 0.05, respectively) compared with the I/R group that did not receive ebselen. Conversely, the 30 mg/kg dose of ebselen increased sciatic nerve damage, apoptosis, iNOS positive cells (P < 0.01, P < 0.05, P < 0.001) and MDA and NO levels (P < 0.05, P < 0.01) and decreased SOD, GPx, and CAT activities (P < 0.05) compared with the sham group. The results of this study suggest that ebselen may cause different effects depending on the dose employed. Ebselen may be protective against sciatic nerve I/R injury via antioxidant and antiapoptotic activities at a 15 mg/kg dose, conversely higher doses may cause detrimental effects.
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| first_indexed | 2024-04-24T23:32:16Z |
| format | Article |
| id | doaj.art-3eb73c83878d4134862b1fba79ba3270 |
| institution | Directory Open Access Journal |
| issn | 2831-0896 2831-090X |
| language | English |
| last_indexed | 2024-04-24T23:32:16Z |
| publishDate | 2015-08-01 |
| publisher | Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina |
| record_format | Article |
| series | Biomolecules & Biomedicine |
| spelling | doaj.art-3eb73c83878d4134862b1fba79ba32702024-03-15T14:32:06ZengAssociation of Basic Medical Sciences of Federation of Bosnia and HerzegovinaBiomolecules & Biomedicine2831-08962831-090X2015-08-0115410.17305/bjbms.2015.52170Different dose-dependent effects of ebselen in sciatic nerve ischemia-reperfusion injury in ratsFiliz Ozyigit0Aysegul Kucuk1Sezer Akcer2Murat Tosun3Emel Fatma Kocak4Cengiz Kocak5Ahmet Kocak6Hasan Metineren7Osman Genc8Dumlupinar University, Faculty of Medicine, Department of Pharmacology, Kutahya, TurkeyDumlupinar University, Faculty of Medicine, Department of Physiology, Kutahya, TurkeyDumlupinar University, Faculty of Medicine, Department of Anatomy, Kutahya, TurkeyAfyon Kocatepe University, Faculty of Medicine, Department of Histology, Afyon, TurkeyDumlupinar University, Faculty of Medicine, Department of Medical Biochemistry, Kutahya, TurkeyDumlupinar University, Faculty of Medicine, Department of Pathology, Kutahya, TurkeyDumlupinar University, Faculty of Medicine, Department of Histology, Kutahya, TurkeyDumlupinar University, Faculty of Medicine, Department of Orthopedics and Traumatology, Kutahya, TurkeyDumlupinar University, Faculty of Medicine, Department of Physiology, Kutahya, Turkey Ebselen is an organoselenium compound which has strong antioxidant and anti-inflammatory effects. We investigated the neuroprotective role of ebselen pretreatment in rats with experimental sciatic nerve ischemia-reperfusion (I/R) injury. Adult male Sprague Dawley rats were divided into four groups (N = 7 in each group). Before sciatic nerve I/R was induced, ebselen was injected intraperitoneally at doses of 15 and 30 mg/kg. After a 2 h ischemia and a 3 h reperfusion period, sciatic nerve tissues were excised. Tissue levels of malondialdehyde (MDA) and nitric oxide (NO), and activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) were measured. Sciatic nerve tissues were also examined histopathologically. The 15 mg/kg dose of ebselen reduced sciatic nerve damage and apoptosis (P < 0.01), levels of MDA, NO, and inducible nitric oxide synthase (iNOS) positive cells (P < 0.01, P < 0.05, respectively), and increased SOD, GPx, and CAT activities (P < 0.001, P < 0.01, P < 0.05, respectively) compared with the I/R group that did not receive ebselen. Conversely, the 30 mg/kg dose of ebselen increased sciatic nerve damage, apoptosis, iNOS positive cells (P < 0.01, P < 0.05, P < 0.001) and MDA and NO levels (P < 0.05, P < 0.01) and decreased SOD, GPx, and CAT activities (P < 0.05) compared with the sham group. The results of this study suggest that ebselen may cause different effects depending on the dose employed. Ebselen may be protective against sciatic nerve I/R injury via antioxidant and antiapoptotic activities at a 15 mg/kg dose, conversely higher doses may cause detrimental effects. https://www.bjbms.org/ojs/index.php/bjbms/article/view/521Ebselenischemia-reperfusion injuryoxidative stresssciatic nerve. |
| spellingShingle | Filiz Ozyigit Aysegul Kucuk Sezer Akcer Murat Tosun Emel Fatma Kocak Cengiz Kocak Ahmet Kocak Hasan Metineren Osman Genc Different dose-dependent effects of ebselen in sciatic nerve ischemia-reperfusion injury in rats Biomolecules & Biomedicine Ebselen ischemia-reperfusion injury oxidative stress sciatic nerve. |
| title | Different dose-dependent effects of ebselen in sciatic nerve ischemia-reperfusion injury in rats |
| title_full | Different dose-dependent effects of ebselen in sciatic nerve ischemia-reperfusion injury in rats |
| title_fullStr | Different dose-dependent effects of ebselen in sciatic nerve ischemia-reperfusion injury in rats |
| title_full_unstemmed | Different dose-dependent effects of ebselen in sciatic nerve ischemia-reperfusion injury in rats |
| title_short | Different dose-dependent effects of ebselen in sciatic nerve ischemia-reperfusion injury in rats |
| title_sort | different dose dependent effects of ebselen in sciatic nerve ischemia reperfusion injury in rats |
| topic | Ebselen ischemia-reperfusion injury oxidative stress sciatic nerve. |
| url | https://www.bjbms.org/ojs/index.php/bjbms/article/view/521 |
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