Phenotypic and Molecular Characterization of Commensal, Community-Acquired and Nosocomial <i>Klebsiella</i> spp.
<i>Klebsiella</i> spp. is a relevant pathogen that can present acquired resistance to almost all available antibiotics, thus representing a serious threat for public health. While most studies have been focused on isolates causing community-acquired and nosocomial infections, little is k...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/11/2344 |
| _version_ | 1797509219779346432 |
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| author | Marta Gómez Arancha Valverde Rosa del Campo Juan Miguel Rodríguez Antonio Maldonado-Barragán |
| author_facet | Marta Gómez Arancha Valverde Rosa del Campo Juan Miguel Rodríguez Antonio Maldonado-Barragán |
| author_sort | Marta Gómez |
| collection | DOAJ |
| description | <i>Klebsiella</i> spp. is a relevant pathogen that can present acquired resistance to almost all available antibiotics, thus representing a serious threat for public health. While most studies have been focused on isolates causing community-acquired and nosocomial infections, little is known about the commensal isolates colonizing healthy subjects. We describe the molecular identification and the phenotypic characterization of commensal <i>Klebsiella</i> spp. from breast milk of healthy women and faeces from healthy breast-fed infants, which were compared with isolates from community-acquired infections and from a nosocomial NICU outbreak. The phylogenetic analysis of a 454-bp sequence of the <i>rpoB</i> gene was useful for species identification (<i>K. pneumoniae</i>, <i>K. variicola</i>, <i>K. quasipneumoniae</i>, <i>K. oxytoca</i>, <i>K. grimontii</i>, <i>K. michiganensis</i>, <i>Raoultella planticola</i> and <i>R. ornithinolytica</i>), previously misidentified as <i>K. pneumoniae</i> or <i>K. oxytoca</i> by biochemical methods. Globally, we report that commensal strains present virulence traits (virulence genes, siderophores and biofilms) comparable to community-acquired and NICU-infective isolates, thus suggesting that the human microbiota could constitute a reservoir for infection. Isolates causing NICU outbreak were multi-drug resistant (MDR) and ESBLs producers, although an imipenem-resistant commensal MDR <i>K. quasipneumoniae</i> isolate was also found. A commensal <i>K. pneumoniae</i> strain showed a potent bacteriocin-like inhibitory activity against MDR <i>Klebsiella</i> isolates, thus highlighting the potential role of commensal <i>Klebsiella</i> spp. in health and disease. |
| first_indexed | 2024-03-10T05:14:37Z |
| format | Article |
| id | doaj.art-3eb7dbd4d08d4ce3a217fbc73ac77e6a |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T05:14:37Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-3eb7dbd4d08d4ce3a217fbc73ac77e6a2023-11-23T00:30:01ZengMDPI AGMicroorganisms2076-26072021-11-01911234410.3390/microorganisms9112344Phenotypic and Molecular Characterization of Commensal, Community-Acquired and Nosocomial <i>Klebsiella</i> spp.Marta Gómez0Arancha Valverde1Rosa del Campo2Juan Miguel Rodríguez3Antonio Maldonado-Barragán4Department of Nutrition and Food Science, Complutense University of Madrid, 28040 Madrid, SpainDepartment of Microbiology, Hospital Universitario Ramón y Cajal IRYCIS, 28034 Madrid, SpainDepartment of Microbiology, Hospital Universitario Ramón y Cajal IRYCIS, 28034 Madrid, SpainDepartment of Nutrition and Food Science, Complutense University of Madrid, 28040 Madrid, SpainDepartment of Nutrition and Food Science, Complutense University of Madrid, 28040 Madrid, Spain<i>Klebsiella</i> spp. is a relevant pathogen that can present acquired resistance to almost all available antibiotics, thus representing a serious threat for public health. While most studies have been focused on isolates causing community-acquired and nosocomial infections, little is known about the commensal isolates colonizing healthy subjects. We describe the molecular identification and the phenotypic characterization of commensal <i>Klebsiella</i> spp. from breast milk of healthy women and faeces from healthy breast-fed infants, which were compared with isolates from community-acquired infections and from a nosocomial NICU outbreak. The phylogenetic analysis of a 454-bp sequence of the <i>rpoB</i> gene was useful for species identification (<i>K. pneumoniae</i>, <i>K. variicola</i>, <i>K. quasipneumoniae</i>, <i>K. oxytoca</i>, <i>K. grimontii</i>, <i>K. michiganensis</i>, <i>Raoultella planticola</i> and <i>R. ornithinolytica</i>), previously misidentified as <i>K. pneumoniae</i> or <i>K. oxytoca</i> by biochemical methods. Globally, we report that commensal strains present virulence traits (virulence genes, siderophores and biofilms) comparable to community-acquired and NICU-infective isolates, thus suggesting that the human microbiota could constitute a reservoir for infection. Isolates causing NICU outbreak were multi-drug resistant (MDR) and ESBLs producers, although an imipenem-resistant commensal MDR <i>K. quasipneumoniae</i> isolate was also found. A commensal <i>K. pneumoniae</i> strain showed a potent bacteriocin-like inhibitory activity against MDR <i>Klebsiella</i> isolates, thus highlighting the potential role of commensal <i>Klebsiella</i> spp. in health and disease.https://www.mdpi.com/2076-2607/9/11/2344<i>Klebsiella</i><i>rpoB</i>virulencesiderophoresbiofilmsantibiotic resistance |
| spellingShingle | Marta Gómez Arancha Valverde Rosa del Campo Juan Miguel Rodríguez Antonio Maldonado-Barragán Phenotypic and Molecular Characterization of Commensal, Community-Acquired and Nosocomial <i>Klebsiella</i> spp. Microorganisms <i>Klebsiella</i> <i>rpoB</i> virulence siderophores biofilms antibiotic resistance |
| title | Phenotypic and Molecular Characterization of Commensal, Community-Acquired and Nosocomial <i>Klebsiella</i> spp. |
| title_full | Phenotypic and Molecular Characterization of Commensal, Community-Acquired and Nosocomial <i>Klebsiella</i> spp. |
| title_fullStr | Phenotypic and Molecular Characterization of Commensal, Community-Acquired and Nosocomial <i>Klebsiella</i> spp. |
| title_full_unstemmed | Phenotypic and Molecular Characterization of Commensal, Community-Acquired and Nosocomial <i>Klebsiella</i> spp. |
| title_short | Phenotypic and Molecular Characterization of Commensal, Community-Acquired and Nosocomial <i>Klebsiella</i> spp. |
| title_sort | phenotypic and molecular characterization of commensal community acquired and nosocomial i klebsiella i spp |
| topic | <i>Klebsiella</i> <i>rpoB</i> virulence siderophores biofilms antibiotic resistance |
| url | https://www.mdpi.com/2076-2607/9/11/2344 |
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