Formation of somatic embryos of plantain cultivar 'FHIA-21' (<em>Musa</em> AAAB) in liquid culture medium
The study aimed to develop somatic embryos (Es) formation of cv. ‘FHIA-21’ (Musa AAAB) in liquid culture medium, from embryogenic cell suspensions. Four inoculation densities (0.5, 1.0, 1.5 and 2.0g of fresh mass (GMF) of embryogenic cell aggregates) in 250 ml Erlenmeyer capacity, which contained 30...
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| Format: | Article |
| Language: | English |
| Published: |
Universidad Central Marta Abreu de Las Villas
2009-04-01
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| Series: | Biotecnología Vegetal |
| Online Access: | https://revista.ibp.co.cu/index.php/BV/article/view/314 |
| _version_ | 1819158484128628736 |
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| author | Leyanis García-Águila Rafael Gómez-Kosky Yelenys Alvarado-Capó Zoe Sarría Maritza Reyes |
| author_facet | Leyanis García-Águila Rafael Gómez-Kosky Yelenys Alvarado-Capó Zoe Sarría Maritza Reyes |
| author_sort | Leyanis García-Águila |
| collection | DOAJ |
| description | The study aimed to develop somatic embryos (Es) formation of cv. ‘FHIA-21’ (Musa AAAB) in liquid culture medium, from embryogenic cell suspensions. Four inoculation densities (0.5, 1.0, 1.5 and 2.0g of fresh mass (GMF) of embryogenic cell aggregates) in 250 ml Erlenmeyer capacity, which contained 30 ml of culture medium were studied. Evaluations were made within 30 days of culture. Tthe following variables were identified: number of Es, length (mm), and morphological and histological features. Results showed the regulatory effect of inoculation density on the formation and morphological development of Es. In this sense, the low density (0.5 and 1.0 GMF) brought about less amount of ES, which were larger and with more advanced ontogenetic development. However, the high density of inoculation (1.5 and 2.0 GMF) produced larger number of uniform size Es and in the globular stage.
Keywords: histology, morphology, liquid culture medium, inoculation density |
| first_indexed | 2024-12-22T16:25:23Z |
| format | Article |
| id | doaj.art-3ebe38acbd304519aaaad8e434920c20 |
| institution | Directory Open Access Journal |
| issn | 1609-1841 2074-8647 |
| language | English |
| last_indexed | 2024-12-22T16:25:23Z |
| publishDate | 2009-04-01 |
| publisher | Universidad Central Marta Abreu de Las Villas |
| record_format | Article |
| series | Biotecnología Vegetal |
| spelling | doaj.art-3ebe38acbd304519aaaad8e434920c202022-12-21T18:20:10ZengUniversidad Central Marta Abreu de Las VillasBiotecnología Vegetal1609-18412074-86472009-04-0192288Formation of somatic embryos of plantain cultivar 'FHIA-21' (<em>Musa</em> AAAB) in liquid culture mediumLeyanis García-ÁguilaRafael Gómez-KoskyYelenys Alvarado-CapóZoe SarríaMaritza ReyesThe study aimed to develop somatic embryos (Es) formation of cv. ‘FHIA-21’ (Musa AAAB) in liquid culture medium, from embryogenic cell suspensions. Four inoculation densities (0.5, 1.0, 1.5 and 2.0g of fresh mass (GMF) of embryogenic cell aggregates) in 250 ml Erlenmeyer capacity, which contained 30 ml of culture medium were studied. Evaluations were made within 30 days of culture. Tthe following variables were identified: number of Es, length (mm), and morphological and histological features. Results showed the regulatory effect of inoculation density on the formation and morphological development of Es. In this sense, the low density (0.5 and 1.0 GMF) brought about less amount of ES, which were larger and with more advanced ontogenetic development. However, the high density of inoculation (1.5 and 2.0 GMF) produced larger number of uniform size Es and in the globular stage. Keywords: histology, morphology, liquid culture medium, inoculation densityhttps://revista.ibp.co.cu/index.php/BV/article/view/314 |
| spellingShingle | Leyanis García-Águila Rafael Gómez-Kosky Yelenys Alvarado-Capó Zoe Sarría Maritza Reyes Formation of somatic embryos of plantain cultivar 'FHIA-21' (<em>Musa</em> AAAB) in liquid culture medium Biotecnología Vegetal |
| title | Formation of somatic embryos of plantain cultivar 'FHIA-21' (<em>Musa</em> AAAB) in liquid culture medium |
| title_full | Formation of somatic embryos of plantain cultivar 'FHIA-21' (<em>Musa</em> AAAB) in liquid culture medium |
| title_fullStr | Formation of somatic embryos of plantain cultivar 'FHIA-21' (<em>Musa</em> AAAB) in liquid culture medium |
| title_full_unstemmed | Formation of somatic embryos of plantain cultivar 'FHIA-21' (<em>Musa</em> AAAB) in liquid culture medium |
| title_short | Formation of somatic embryos of plantain cultivar 'FHIA-21' (<em>Musa</em> AAAB) in liquid culture medium |
| title_sort | formation of somatic embryos of plantain cultivar fhia 21 em musa em aaab in liquid culture medium |
| url | https://revista.ibp.co.cu/index.php/BV/article/view/314 |
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