Ascochyta blight (Ascochyta fabae) of faba bean (Vicia faba L.): Phenotypic and molecular characterization, pathogenicity and in vitro biological control by Bacillus spp. and Pseudomonas spp.
Ascochyta blight is a major biotic constraint of faba bean (Vicia faba L.) production and productivity worldwide caused by Ascochyta fabae. No studies have been performed in Algeria to identify A. fabae isolates or to assess their pathogenicity, and therefore information on local isolates i...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
University of Belgrade, University of Novi Sad
2023-01-01
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| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | https://doiserbia.nb.rs/img/doi/0354-4664/2023/0354-46642300009O.pdf |
| Summary: | Ascochyta blight is a major biotic constraint of faba bean (Vicia faba L.)
production and productivity worldwide caused by Ascochyta fabae. No studies
have been performed in Algeria to identify A. fabae isolates or to assess
their pathogenicity, and therefore information on local isolates is
insufficient. Herein, 14 isolates of A. fabae were obtained from faba bean
samples and identified based on morphological traits and phylogenetic
analysis of internal transcribed spacer sequences. All generated sequences
have been deposited in GenBank and assigned accession numbers. Pathogenicity
tests on faba bean plants revealed that all isolates produced necrotic
lesions on aerial parts with variable intensity, classifying them as weakly,
moderately, and highly aggressive. The in vitro antifungal activity of
Bacillus and Pseudomonas strains against A. fabae isolates showed that
Bacillus siamensis B8 and Bacillus mojavensis B31 had the highest
suppressive potential against all isolates. Moreover, a negative correlation
was found between the aggressiveness of A. fabae isolates and their
susceptibility to biocontrol strains. This is the first report on the
identification, pathogenicity and in vitro biological control of A. fabae
isolates in Algeria. B8 and B31 could be further developed as promising
biocontrol agents for the control of the ascochyta blight of faba bean. |
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| ISSN: | 0354-4664 1821-4339 |