Ascochyta blight (Ascochyta fabae) of faba bean (Vicia faba L.): Phenotypic and molecular characterization, pathogenicity and in vitro biological control by Bacillus spp. and Pseudomonas spp.
Ascochyta blight is a major biotic constraint of faba bean (Vicia faba L.) production and productivity worldwide caused by Ascochyta fabae. No studies have been performed in Algeria to identify A. fabae isolates or to assess their pathogenicity, and therefore information on local isolates i...
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| Format: | Article |
| Language: | English |
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University of Belgrade, University of Novi Sad
2023-01-01
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| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | https://doiserbia.nb.rs/img/doi/0354-4664/2023/0354-46642300009O.pdf |
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| author | Oguiba Bouchra Karkachi Noureddine Suárez-Estrella Francisca Haouhach Sadika Kihal Mebrouk López María J. |
| author_facet | Oguiba Bouchra Karkachi Noureddine Suárez-Estrella Francisca Haouhach Sadika Kihal Mebrouk López María J. |
| author_sort | Oguiba Bouchra |
| collection | DOAJ |
| description | Ascochyta blight is a major biotic constraint of faba bean (Vicia faba L.)
production and productivity worldwide caused by Ascochyta fabae. No studies
have been performed in Algeria to identify A. fabae isolates or to assess
their pathogenicity, and therefore information on local isolates is
insufficient. Herein, 14 isolates of A. fabae were obtained from faba bean
samples and identified based on morphological traits and phylogenetic
analysis of internal transcribed spacer sequences. All generated sequences
have been deposited in GenBank and assigned accession numbers. Pathogenicity
tests on faba bean plants revealed that all isolates produced necrotic
lesions on aerial parts with variable intensity, classifying them as weakly,
moderately, and highly aggressive. The in vitro antifungal activity of
Bacillus and Pseudomonas strains against A. fabae isolates showed that
Bacillus siamensis B8 and Bacillus mojavensis B31 had the highest
suppressive potential against all isolates. Moreover, a negative correlation
was found between the aggressiveness of A. fabae isolates and their
susceptibility to biocontrol strains. This is the first report on the
identification, pathogenicity and in vitro biological control of A. fabae
isolates in Algeria. B8 and B31 could be further developed as promising
biocontrol agents for the control of the ascochyta blight of faba bean. |
| first_indexed | 2024-03-13T06:24:33Z |
| format | Article |
| id | doaj.art-3ebf86ec4c974acc8015ee3c6b040e77 |
| institution | Directory Open Access Journal |
| issn | 0354-4664 1821-4339 |
| language | English |
| last_indexed | 2024-03-13T06:24:33Z |
| publishDate | 2023-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-3ebf86ec4c974acc8015ee3c6b040e772023-06-09T10:41:59ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392023-01-0175110311710.2298/ABS230203009O0354-46642300009OAscochyta blight (Ascochyta fabae) of faba bean (Vicia faba L.): Phenotypic and molecular characterization, pathogenicity and in vitro biological control by Bacillus spp. and Pseudomonas spp.Oguiba Bouchra0Karkachi Noureddine1Suárez-Estrella Francisca2Haouhach Sadika3Kihal Mebrouk4López María J.5Laboratory of Applied Microbiology, Department of Biology, Faculty of Natural and Life Sciences, University of Oran Ahmed Ben Bella, Oran, AlgeriaLaboratory of Applied Microbiology, Department of Biology, Faculty of Natural and Life Sciences, University of Oran Ahmed Ben Bella, Oran, AlgeriaDepartment of Biology and Geology, University of Almería, Agrifood Campus of International Excellence (ceiA), Center for Research in Mediterranean Intensive Agrosystems and Agri- Food Biotechnology (CIAIMBITAL), Almería, SpainDepartment of Biotechnology, University of Science and Technology of Oran Mohamed Boudiaf, Oran, AlgeriaLaboratory of Applied Microbiology, Department of Biology, Faculty of Natural and Life Sciences, University of Oran Ahmed Ben Bella, Oran, AlgeriaDepartment of Biology and Geology, University of Almería, Agrifood Campus of International Excellence (ceiA), Center for Research in Mediterranean Intensive Agrosystems and Agri- Food Biotechnology (CIAIMBITAL), Almería, SpainAscochyta blight is a major biotic constraint of faba bean (Vicia faba L.) production and productivity worldwide caused by Ascochyta fabae. No studies have been performed in Algeria to identify A. fabae isolates or to assess their pathogenicity, and therefore information on local isolates is insufficient. Herein, 14 isolates of A. fabae were obtained from faba bean samples and identified based on morphological traits and phylogenetic analysis of internal transcribed spacer sequences. All generated sequences have been deposited in GenBank and assigned accession numbers. Pathogenicity tests on faba bean plants revealed that all isolates produced necrotic lesions on aerial parts with variable intensity, classifying them as weakly, moderately, and highly aggressive. The in vitro antifungal activity of Bacillus and Pseudomonas strains against A. fabae isolates showed that Bacillus siamensis B8 and Bacillus mojavensis B31 had the highest suppressive potential against all isolates. Moreover, a negative correlation was found between the aggressiveness of A. fabae isolates and their susceptibility to biocontrol strains. This is the first report on the identification, pathogenicity and in vitro biological control of A. fabae isolates in Algeria. B8 and B31 could be further developed as promising biocontrol agents for the control of the ascochyta blight of faba bean.https://doiserbia.nb.rs/img/doi/0354-4664/2023/0354-46642300009O.pdfascochyta blightascochyta fabaebiological controlvicia faba l.faba beanpathogenicity |
| spellingShingle | Oguiba Bouchra Karkachi Noureddine Suárez-Estrella Francisca Haouhach Sadika Kihal Mebrouk López María J. Ascochyta blight (Ascochyta fabae) of faba bean (Vicia faba L.): Phenotypic and molecular characterization, pathogenicity and in vitro biological control by Bacillus spp. and Pseudomonas spp. Archives of Biological Sciences ascochyta blight ascochyta fabae biological control vicia faba l. faba bean pathogenicity |
| title | Ascochyta blight (Ascochyta fabae) of faba bean (Vicia faba L.): Phenotypic and molecular characterization, pathogenicity and in vitro biological control by Bacillus spp. and Pseudomonas spp. |
| title_full | Ascochyta blight (Ascochyta fabae) of faba bean (Vicia faba L.): Phenotypic and molecular characterization, pathogenicity and in vitro biological control by Bacillus spp. and Pseudomonas spp. |
| title_fullStr | Ascochyta blight (Ascochyta fabae) of faba bean (Vicia faba L.): Phenotypic and molecular characterization, pathogenicity and in vitro biological control by Bacillus spp. and Pseudomonas spp. |
| title_full_unstemmed | Ascochyta blight (Ascochyta fabae) of faba bean (Vicia faba L.): Phenotypic and molecular characterization, pathogenicity and in vitro biological control by Bacillus spp. and Pseudomonas spp. |
| title_short | Ascochyta blight (Ascochyta fabae) of faba bean (Vicia faba L.): Phenotypic and molecular characterization, pathogenicity and in vitro biological control by Bacillus spp. and Pseudomonas spp. |
| title_sort | ascochyta blight ascochyta fabae of faba bean vicia faba l phenotypic and molecular characterization pathogenicity and in vitro biological control by bacillus spp and pseudomonas spp |
| topic | ascochyta blight ascochyta fabae biological control vicia faba l. faba bean pathogenicity |
| url | https://doiserbia.nb.rs/img/doi/0354-4664/2023/0354-46642300009O.pdf |
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