Pregnancy-Induced Alterations in NK Cell Phenotype and Function
Pregnant women are particularly susceptible to complications of influenza A virus infection, which may result from pregnancy-induced changes in the function of immune cells, including natural killer (NK) cells. To better understand NK cell function during pregnancy, we assessed the ability of the tw...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-10-01
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| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fimmu.2019.02469/full |
| _version_ | 1819062251950178304 |
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| author | Mathieu Le Gars Mathieu Le Gars Christof Seiler Alexander W. Kay Nicholas L. Bayless Elina Starosvetsky Lindsay Moore Shai S. Shen-Orr Natali Aziz Purvesh Khatri Cornelia L. Dekker Gary E. Swan Mark M. Davis Mark M. Davis Susan Holmes Catherine A. Blish Catherine A. Blish Catherine A. Blish |
| author_facet | Mathieu Le Gars Mathieu Le Gars Christof Seiler Alexander W. Kay Nicholas L. Bayless Elina Starosvetsky Lindsay Moore Shai S. Shen-Orr Natali Aziz Purvesh Khatri Cornelia L. Dekker Gary E. Swan Mark M. Davis Mark M. Davis Susan Holmes Catherine A. Blish Catherine A. Blish Catherine A. Blish |
| author_sort | Mathieu Le Gars |
| collection | DOAJ |
| description | Pregnant women are particularly susceptible to complications of influenza A virus infection, which may result from pregnancy-induced changes in the function of immune cells, including natural killer (NK) cells. To better understand NK cell function during pregnancy, we assessed the ability of the two main subsets of NK cells, CD56dim, and CD56bright NK cells, to respond to influenza-virus infected cells and tumor cells. During pregnancy, CD56dim and CD56bright NK cells displayed enhanced functional responses to both infected and tumor cells, with increased expression of degranulation markers and elevated frequency of NK cells producing IFN-γ. To better understand the mechanisms driving this enhanced function, we profiled CD56dim and CD56bright NK cells from pregnant and non-pregnant women using mass cytometry. NK cells from pregnant women displayed significantly increased expression of several functional and activation markers such as CD38 on both subsets and NKp46 on CD56dim NK cells. NK cells also displayed diminished expression of the chemokine receptor CXCR3 during pregnancy. Overall, these data demonstrate that functional and phenotypic shifts occur in NK cells during pregnancy that can influence the magnitude of the immune response to both infections and tumors. |
| first_indexed | 2024-12-21T14:55:49Z |
| format | Article |
| id | doaj.art-3ebfba4f605d4fb48eb479e2c9e2771d |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-21T14:55:49Z |
| publishDate | 2019-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-3ebfba4f605d4fb48eb479e2c9e2771d2022-12-21T18:59:46ZengFrontiers Media S.A.Frontiers in Immunology1664-32242019-10-011010.3389/fimmu.2019.02469474193Pregnancy-Induced Alterations in NK Cell Phenotype and FunctionMathieu Le Gars0Mathieu Le Gars1Christof Seiler2Alexander W. Kay3Nicholas L. Bayless4Elina Starosvetsky5Lindsay Moore6Shai S. Shen-Orr7Natali Aziz8Purvesh Khatri9Cornelia L. Dekker10Gary E. Swan11Mark M. Davis12Mark M. Davis13Susan Holmes14Catherine A. Blish15Catherine A. Blish16Catherine A. Blish17Department of Medicine, Stanford University, Palo Alto, CA, United StatesDepartment of Stanford Immunology Program, Stanford University, Palo Alto, CA, United StatesDepartment of Statistics, Stanford University, Palo Alto, CA, United StatesDepartment of Pediatrics, Stanford University, Palo Alto, CA, United StatesDepartment of Stanford Immunology Program, Stanford University, Palo Alto, CA, United StatesFaculty of Medicine, Technion - Israel Institute of Technology, Haifa, IsraelFaculty of Medicine, Technion - Israel Institute of Technology, Haifa, IsraelFaculty of Medicine, Technion - Israel Institute of Technology, Haifa, IsraelDepartment of Obstetrics and Gynecology, Stanford Prevention Research Center, Stanford University School of Medicine, Stanford University, Palo Alto, CA, United StatesDepartment of Medicine, Stanford University, Palo Alto, CA, United StatesDepartment of Statistics, Stanford University, Palo Alto, CA, United StatesDepartment of Obstetrics and Gynecology, Stanford Prevention Research Center, Stanford University School of Medicine, Stanford University, Palo Alto, CA, United StatesDepartment of Microbiology and Immunology, Stanford University, Palo Alto, CA, United StatesHoward Hughes Medical Institute, Stanford University School of Medicine, Stanford, CA, United StatesDepartment of Pediatrics, Stanford University, Palo Alto, CA, United StatesDepartment of Medicine, Stanford University, Palo Alto, CA, United StatesDepartment of Stanford Immunology Program, Stanford University, Palo Alto, CA, United StatesChan Zuckerberg Biohub, San Francisco, CA, United StatesPregnant women are particularly susceptible to complications of influenza A virus infection, which may result from pregnancy-induced changes in the function of immune cells, including natural killer (NK) cells. To better understand NK cell function during pregnancy, we assessed the ability of the two main subsets of NK cells, CD56dim, and CD56bright NK cells, to respond to influenza-virus infected cells and tumor cells. During pregnancy, CD56dim and CD56bright NK cells displayed enhanced functional responses to both infected and tumor cells, with increased expression of degranulation markers and elevated frequency of NK cells producing IFN-γ. To better understand the mechanisms driving this enhanced function, we profiled CD56dim and CD56bright NK cells from pregnant and non-pregnant women using mass cytometry. NK cells from pregnant women displayed significantly increased expression of several functional and activation markers such as CD38 on both subsets and NKp46 on CD56dim NK cells. NK cells also displayed diminished expression of the chemokine receptor CXCR3 during pregnancy. Overall, these data demonstrate that functional and phenotypic shifts occur in NK cells during pregnancy that can influence the magnitude of the immune response to both infections and tumors.https://www.frontiersin.org/article/10.3389/fimmu.2019.02469/fullNK cellspregnancyinfluenza viruscancer cellsNK repertoire |
| spellingShingle | Mathieu Le Gars Mathieu Le Gars Christof Seiler Alexander W. Kay Nicholas L. Bayless Elina Starosvetsky Lindsay Moore Shai S. Shen-Orr Natali Aziz Purvesh Khatri Cornelia L. Dekker Gary E. Swan Mark M. Davis Mark M. Davis Susan Holmes Catherine A. Blish Catherine A. Blish Catherine A. Blish Pregnancy-Induced Alterations in NK Cell Phenotype and Function Frontiers in Immunology NK cells pregnancy influenza virus cancer cells NK repertoire |
| title | Pregnancy-Induced Alterations in NK Cell Phenotype and Function |
| title_full | Pregnancy-Induced Alterations in NK Cell Phenotype and Function |
| title_fullStr | Pregnancy-Induced Alterations in NK Cell Phenotype and Function |
| title_full_unstemmed | Pregnancy-Induced Alterations in NK Cell Phenotype and Function |
| title_short | Pregnancy-Induced Alterations in NK Cell Phenotype and Function |
| title_sort | pregnancy induced alterations in nk cell phenotype and function |
| topic | NK cells pregnancy influenza virus cancer cells NK repertoire |
| url | https://www.frontiersin.org/article/10.3389/fimmu.2019.02469/full |
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