Genetic polymorphism of merozoite surface proteins 1 and 2 of Plasmodium falciparum in the China–Myanmar border region
Abstract Background Malaria is a major public health problem in the China–Myanmar border region. The genetic structure of malaria parasite may affect its transmission model and control strategies. The present study was to analyse genetic diversity of Plasmodium falciparum by merozoite surface protei...
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| Format: | Article |
| Language: | English |
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BMC
2019-11-01
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| Series: | Malaria Journal |
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| Online Access: | http://link.springer.com/article/10.1186/s12936-019-3003-8 |
| _version_ | 1818401074848989184 |
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| author | Cang-Lin Zhang Hong-Ning Zhou Quan Liu Ya-Ming Yang |
| author_facet | Cang-Lin Zhang Hong-Ning Zhou Quan Liu Ya-Ming Yang |
| author_sort | Cang-Lin Zhang |
| collection | DOAJ |
| description | Abstract Background Malaria is a major public health problem in the China–Myanmar border region. The genetic structure of malaria parasite may affect its transmission model and control strategies. The present study was to analyse genetic diversity of Plasmodium falciparum by merozoite surface proteins 1 and 2 (MSP1 and MSP2) and to determine the multiplicity of infection in clinical isolates in the China–Myanmar border region. Methods Venous blood samples (172) and filter paper blood spots (70) of P. falciparum isolates were collected from the patients of the China–Myanmar border region from 2006 to 2011. The genomic DNA was extracted, and the msp1 and msp2 genes were genotyped by nested PCR using allele-specific primers for P. falciparum. Results A total of 215 P. falciparum clinical isolates were genotyped at the msp1 (201) and msp2 (204), respectively. For the msp1 gene, MAD20 family was dominant (53.49%), followed by the K1 family (44.65%), and the RO33 family (12.56%). For the msp2 gene, the most frequent allele was the FC27 family (80.93%), followed by the 3D7 family (75.81%). The total multiplicity of infection (MOI) of msp1 and msp2 was 1.76 and 2.21, with a prevalence of 64.19% and 72.09%, respectively. A significant positive correlation between the MOI and parasite density was found in the msp1 gene of P. falciparum. Sequence analysis revealed 38 different alleles of msp1 (14 K1, 23 MAD20, and 1 RO33) and 52 different alleles of msp2 (37 3D7 and 15 FC27). Conclusion The present study showed the genetic polymorphisms with diverse allele types of msp1 and msp2 as well as the high MOI of P. falciparum clinical isolates in the China–Myanmar border region. |
| first_indexed | 2024-12-14T07:46:41Z |
| format | Article |
| id | doaj.art-3ec02d2ffc23452ea939a5ad8735d7a6 |
| institution | Directory Open Access Journal |
| issn | 1475-2875 |
| language | English |
| last_indexed | 2024-12-14T07:46:41Z |
| publishDate | 2019-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Malaria Journal |
| spelling | doaj.art-3ec02d2ffc23452ea939a5ad8735d7a62022-12-21T23:10:53ZengBMCMalaria Journal1475-28752019-11-0118111010.1186/s12936-019-3003-8Genetic polymorphism of merozoite surface proteins 1 and 2 of Plasmodium falciparum in the China–Myanmar border regionCang-Lin Zhang0Hong-Ning Zhou1Quan Liu2Ya-Ming Yang3Yunnan Provincial Key Laboratory of Vector-borne Diseases Control and Research, Yunnan Provincial Center of Malaria Research, Yunnan Institute of Parasitic DiseasesYunnan Provincial Key Laboratory of Vector-borne Diseases Control and Research, Yunnan Provincial Center of Malaria Research, Yunnan Institute of Parasitic DiseasesSchool of Life Sciences and Engineering, Foshan UniversityYunnan Provincial Key Laboratory of Vector-borne Diseases Control and Research, Yunnan Provincial Center of Malaria Research, Yunnan Institute of Parasitic DiseasesAbstract Background Malaria is a major public health problem in the China–Myanmar border region. The genetic structure of malaria parasite may affect its transmission model and control strategies. The present study was to analyse genetic diversity of Plasmodium falciparum by merozoite surface proteins 1 and 2 (MSP1 and MSP2) and to determine the multiplicity of infection in clinical isolates in the China–Myanmar border region. Methods Venous blood samples (172) and filter paper blood spots (70) of P. falciparum isolates were collected from the patients of the China–Myanmar border region from 2006 to 2011. The genomic DNA was extracted, and the msp1 and msp2 genes were genotyped by nested PCR using allele-specific primers for P. falciparum. Results A total of 215 P. falciparum clinical isolates were genotyped at the msp1 (201) and msp2 (204), respectively. For the msp1 gene, MAD20 family was dominant (53.49%), followed by the K1 family (44.65%), and the RO33 family (12.56%). For the msp2 gene, the most frequent allele was the FC27 family (80.93%), followed by the 3D7 family (75.81%). The total multiplicity of infection (MOI) of msp1 and msp2 was 1.76 and 2.21, with a prevalence of 64.19% and 72.09%, respectively. A significant positive correlation between the MOI and parasite density was found in the msp1 gene of P. falciparum. Sequence analysis revealed 38 different alleles of msp1 (14 K1, 23 MAD20, and 1 RO33) and 52 different alleles of msp2 (37 3D7 and 15 FC27). Conclusion The present study showed the genetic polymorphisms with diverse allele types of msp1 and msp2 as well as the high MOI of P. falciparum clinical isolates in the China–Myanmar border region.http://link.springer.com/article/10.1186/s12936-019-3003-8Plasmodium falciparumMerozoite surface proteinGenetic polymorphismThe China–Myanmar border region |
| spellingShingle | Cang-Lin Zhang Hong-Ning Zhou Quan Liu Ya-Ming Yang Genetic polymorphism of merozoite surface proteins 1 and 2 of Plasmodium falciparum in the China–Myanmar border region Malaria Journal Plasmodium falciparum Merozoite surface protein Genetic polymorphism The China–Myanmar border region |
| title | Genetic polymorphism of merozoite surface proteins 1 and 2 of Plasmodium falciparum in the China–Myanmar border region |
| title_full | Genetic polymorphism of merozoite surface proteins 1 and 2 of Plasmodium falciparum in the China–Myanmar border region |
| title_fullStr | Genetic polymorphism of merozoite surface proteins 1 and 2 of Plasmodium falciparum in the China–Myanmar border region |
| title_full_unstemmed | Genetic polymorphism of merozoite surface proteins 1 and 2 of Plasmodium falciparum in the China–Myanmar border region |
| title_short | Genetic polymorphism of merozoite surface proteins 1 and 2 of Plasmodium falciparum in the China–Myanmar border region |
| title_sort | genetic polymorphism of merozoite surface proteins 1 and 2 of plasmodium falciparum in the china myanmar border region |
| topic | Plasmodium falciparum Merozoite surface protein Genetic polymorphism The China–Myanmar border region |
| url | http://link.springer.com/article/10.1186/s12936-019-3003-8 |
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