PDGFBB improved the biological function of menstrual blood-derived stromal cells and the anti-fibrotic properties of exosomes
Abstract Background Intrauterine adhesion (IUA) is a reproductive dysfunction disease characterized by endometrial fibrosis, with limited therapeutic options and poor prognosis. Our previous studies confirmed that menstrual blood-derived stromal cells (MenSCs) effectively attenuated endometrial fibr...
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| Format: | Article |
| Language: | English |
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BMC
2023-04-01
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| Series: | Stem Cell Research & Therapy |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13287-023-03339-y |
| _version_ | 1797836605579329536 |
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| author | Xudong Zhang Siwen Zhang Jiarui Qi Fujie Zhao Yimeng Lu Shuyu Li Shanshan Wu Pingping Li Jichun Tan |
| author_facet | Xudong Zhang Siwen Zhang Jiarui Qi Fujie Zhao Yimeng Lu Shuyu Li Shanshan Wu Pingping Li Jichun Tan |
| author_sort | Xudong Zhang |
| collection | DOAJ |
| description | Abstract Background Intrauterine adhesion (IUA) is a reproductive dysfunction disease characterized by endometrial fibrosis, with limited therapeutic options and poor prognosis. Our previous studies confirmed that menstrual blood-derived stromal cells (MenSCs) effectively attenuated endometrial fibrosis in an animal model of IUA mainly through exosomes. This therapeutic effect can be enhanced by platelet-rich plasma (PRP), in which PDGFBB is an abundant growth factor. Therefore, we aimed to compare the effects of PRP and PDGFBB on the biological activities of MenSCs in vitro, and to further investigate the molecular mechanism of MenSCs-derived exosomes in alleviating endometrial fibrosis. Methods MenSCs were isolated for in vitro functional assays to examine the viability, migration, and stemness of MenSCs. Endometrial stromal cells (EndoSCs) were treated with 50 ug/ml of MenSCs-derived exosomes, obtained by differential ultracentrifugation extraction. The molecular mechanisms by which PDGFBB improves MenSCs and exosomes alleviate EndoSCs fibrosis were then explored using immunofluorescence, western blot, and co-immunoprecipitation. Results Both 100 ng/ml PDGFBB and 10% activated PRP promoted the proliferation, increased the S phase of cell cycle, and inhibited apoptosis of MenSCs in vitro. Compared with PRP, PDGFBB significantly promoted MenSCs migration. All of these effects were inhibited by sorafenib, a PDGFR-β inhibitor. PRP and PDGFBB activated AKT/NF-κB signaling pathway in MenSCs and increased the expression of P65 and OCT4. Moreover, pretreatment of PDGFBB did not increase the secretion of MenSCs but significantly increased the anti-fibrosis effects of MenSCs-derived exosomes on IUA-EndoSCs. MenSCs-derived exosomes attenuated SMAD3 phosphorylation and increased YAP ubiquitination, which reduced the binding of YAP/SMAD3. Pretreatment with PDGFBB amplified this effect. Conclusions In summary, PDGFBB could improve the biological functions of MenSCs via AKT/NF-κB signaling pathway, including viability, migration, and stemness. Our results indicated that PDGFBB amplified MenSCs-derived exosomes to attenuate endometrial fibrosis by inhibiting YAP activity, revealing a novel mechanism by which PRP enhanced the ability of MenSCs to repair tissue injury and providing a potential option for improving stem cell efficacy in IUA. |
| first_indexed | 2024-04-09T15:11:37Z |
| format | Article |
| id | doaj.art-3ec181609f1e44eba846aead86f4539f |
| institution | Directory Open Access Journal |
| issn | 1757-6512 |
| language | English |
| last_indexed | 2024-04-09T15:11:37Z |
| publishDate | 2023-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Stem Cell Research & Therapy |
| spelling | doaj.art-3ec181609f1e44eba846aead86f4539f2023-04-30T11:09:06ZengBMCStem Cell Research & Therapy1757-65122023-04-0114111410.1186/s13287-023-03339-yPDGFBB improved the biological function of menstrual blood-derived stromal cells and the anti-fibrotic properties of exosomesXudong Zhang0Siwen Zhang1Jiarui Qi2Fujie Zhao3Yimeng Lu4Shuyu Li5Shanshan Wu6Pingping Li7Jichun Tan8Center of Reproductive Medicine, Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical UniversityCenter of Reproductive Medicine, Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical UniversityCenter of Reproductive Medicine, Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical UniversityObstetrics and Gynecology Department, Shengjing Hospital of China Medical UniversityCenter of Reproductive Medicine, Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical UniversityCenter of Reproductive Medicine, Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical UniversityCenter of Reproductive Medicine, Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical UniversityCenter of Reproductive Medicine, Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical UniversityCenter of Reproductive Medicine, Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical UniversityAbstract Background Intrauterine adhesion (IUA) is a reproductive dysfunction disease characterized by endometrial fibrosis, with limited therapeutic options and poor prognosis. Our previous studies confirmed that menstrual blood-derived stromal cells (MenSCs) effectively attenuated endometrial fibrosis in an animal model of IUA mainly through exosomes. This therapeutic effect can be enhanced by platelet-rich plasma (PRP), in which PDGFBB is an abundant growth factor. Therefore, we aimed to compare the effects of PRP and PDGFBB on the biological activities of MenSCs in vitro, and to further investigate the molecular mechanism of MenSCs-derived exosomes in alleviating endometrial fibrosis. Methods MenSCs were isolated for in vitro functional assays to examine the viability, migration, and stemness of MenSCs. Endometrial stromal cells (EndoSCs) were treated with 50 ug/ml of MenSCs-derived exosomes, obtained by differential ultracentrifugation extraction. The molecular mechanisms by which PDGFBB improves MenSCs and exosomes alleviate EndoSCs fibrosis were then explored using immunofluorescence, western blot, and co-immunoprecipitation. Results Both 100 ng/ml PDGFBB and 10% activated PRP promoted the proliferation, increased the S phase of cell cycle, and inhibited apoptosis of MenSCs in vitro. Compared with PRP, PDGFBB significantly promoted MenSCs migration. All of these effects were inhibited by sorafenib, a PDGFR-β inhibitor. PRP and PDGFBB activated AKT/NF-κB signaling pathway in MenSCs and increased the expression of P65 and OCT4. Moreover, pretreatment of PDGFBB did not increase the secretion of MenSCs but significantly increased the anti-fibrosis effects of MenSCs-derived exosomes on IUA-EndoSCs. MenSCs-derived exosomes attenuated SMAD3 phosphorylation and increased YAP ubiquitination, which reduced the binding of YAP/SMAD3. Pretreatment with PDGFBB amplified this effect. Conclusions In summary, PDGFBB could improve the biological functions of MenSCs via AKT/NF-κB signaling pathway, including viability, migration, and stemness. Our results indicated that PDGFBB amplified MenSCs-derived exosomes to attenuate endometrial fibrosis by inhibiting YAP activity, revealing a novel mechanism by which PRP enhanced the ability of MenSCs to repair tissue injury and providing a potential option for improving stem cell efficacy in IUA.https://doi.org/10.1186/s13287-023-03339-yMenSCsPDGFBBExosomesFibrosisYAPIntrauterine adhesion |
| spellingShingle | Xudong Zhang Siwen Zhang Jiarui Qi Fujie Zhao Yimeng Lu Shuyu Li Shanshan Wu Pingping Li Jichun Tan PDGFBB improved the biological function of menstrual blood-derived stromal cells and the anti-fibrotic properties of exosomes Stem Cell Research & Therapy MenSCs PDGFBB Exosomes Fibrosis YAP Intrauterine adhesion |
| title | PDGFBB improved the biological function of menstrual blood-derived stromal cells and the anti-fibrotic properties of exosomes |
| title_full | PDGFBB improved the biological function of menstrual blood-derived stromal cells and the anti-fibrotic properties of exosomes |
| title_fullStr | PDGFBB improved the biological function of menstrual blood-derived stromal cells and the anti-fibrotic properties of exosomes |
| title_full_unstemmed | PDGFBB improved the biological function of menstrual blood-derived stromal cells and the anti-fibrotic properties of exosomes |
| title_short | PDGFBB improved the biological function of menstrual blood-derived stromal cells and the anti-fibrotic properties of exosomes |
| title_sort | pdgfbb improved the biological function of menstrual blood derived stromal cells and the anti fibrotic properties of exosomes |
| topic | MenSCs PDGFBB Exosomes Fibrosis YAP Intrauterine adhesion |
| url | https://doi.org/10.1186/s13287-023-03339-y |
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