Using Serum Specimens for Real-Time PCR-Based Diagnosis of Human Granulocytic Anaplasmosis, Canada
Whole blood is the optimal specimen for anaplasmosis diagnosis but might not be available in all cases. We PCR tested serum samples collected in Canada for Anaplasma serology and found 84.8%–95.8% sensitivity and 2.8 average cycle threshold elevation. Serum can be acceptable for detecting Anaplasma...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Centers for Disease Control and Prevention
2023-01-01
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| Series: | Emerging Infectious Diseases |
| Subjects: | |
| Online Access: | https://wwwnc.cdc.gov/eid/article/29/1/22-0988_article |
| _version_ | 1797959723344986112 |
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| author | Carl Boodman Courtney Loomer Antonia Dibernardo Todd Hatchette Jason J. LeBlanc Brooks Waitt L. Robbin Lindsay |
| author_facet | Carl Boodman Courtney Loomer Antonia Dibernardo Todd Hatchette Jason J. LeBlanc Brooks Waitt L. Robbin Lindsay |
| author_sort | Carl Boodman |
| collection | DOAJ |
| description |
Whole blood is the optimal specimen for anaplasmosis diagnosis but might not be available in all cases. We PCR tested serum samples collected in Canada for Anaplasma serology and found 84.8%–95.8% sensitivity and 2.8 average cycle threshold elevation. Serum can be acceptable for detecting Anaplasma spp. when whole blood is unavailable.
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| first_indexed | 2024-04-11T00:35:45Z |
| format | Article |
| id | doaj.art-3ec2113850924b79902b14bf8f44716f |
| institution | Directory Open Access Journal |
| issn | 1080-6040 1080-6059 |
| language | English |
| last_indexed | 2024-04-11T00:35:45Z |
| publishDate | 2023-01-01 |
| publisher | Centers for Disease Control and Prevention |
| record_format | Article |
| series | Emerging Infectious Diseases |
| spelling | doaj.art-3ec2113850924b79902b14bf8f44716f2023-01-06T17:27:35ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592023-01-0129117517810.3201/eid2901.220988Using Serum Specimens for Real-Time PCR-Based Diagnosis of Human Granulocytic Anaplasmosis, CanadaCarl BoodmanCourtney LoomerAntonia DibernardoTodd HatchetteJason J. LeBlancBrooks WaittL. Robbin Lindsay Whole blood is the optimal specimen for anaplasmosis diagnosis but might not be available in all cases. We PCR tested serum samples collected in Canada for Anaplasma serology and found 84.8%–95.8% sensitivity and 2.8 average cycle threshold elevation. Serum can be acceptable for detecting Anaplasma spp. when whole blood is unavailable. https://wwwnc.cdc.gov/eid/article/29/1/22-0988_articleanaplasmosisbacteriavector-borne infectionszoonosesAnaplasma phagocytophilumemerging infections |
| spellingShingle | Carl Boodman Courtney Loomer Antonia Dibernardo Todd Hatchette Jason J. LeBlanc Brooks Waitt L. Robbin Lindsay Using Serum Specimens for Real-Time PCR-Based Diagnosis of Human Granulocytic Anaplasmosis, Canada Emerging Infectious Diseases anaplasmosis bacteria vector-borne infections zoonoses Anaplasma phagocytophilum emerging infections |
| title | Using Serum Specimens for Real-Time PCR-Based Diagnosis of Human Granulocytic Anaplasmosis, Canada |
| title_full | Using Serum Specimens for Real-Time PCR-Based Diagnosis of Human Granulocytic Anaplasmosis, Canada |
| title_fullStr | Using Serum Specimens for Real-Time PCR-Based Diagnosis of Human Granulocytic Anaplasmosis, Canada |
| title_full_unstemmed | Using Serum Specimens for Real-Time PCR-Based Diagnosis of Human Granulocytic Anaplasmosis, Canada |
| title_short | Using Serum Specimens for Real-Time PCR-Based Diagnosis of Human Granulocytic Anaplasmosis, Canada |
| title_sort | using serum specimens for real time pcr based diagnosis of human granulocytic anaplasmosis canada |
| topic | anaplasmosis bacteria vector-borne infections zoonoses Anaplasma phagocytophilum emerging infections |
| url | https://wwwnc.cdc.gov/eid/article/29/1/22-0988_article |
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