Identification of Volatile Sulfur Compounds Produced by <i>Schizophyllum commune</i>
<i>Schizophyllum commune</i> is a causative agent of allergic bronchopulmonary mycosis, allergic fungal rhinosinusitis, and basidiomycosis. Diagnosis of these diseases remains difficult because no commercially available tool exists to identify the pathogen. Unique volatile organic compou...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-06-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/7/6/465 |
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| author | Takahito Toyotome Masahiko Takino Masahiro Takaya Maki Yahiro Katsuhiko Kamei |
| author_facet | Takahito Toyotome Masahiko Takino Masahiro Takaya Maki Yahiro Katsuhiko Kamei |
| author_sort | Takahito Toyotome |
| collection | DOAJ |
| description | <i>Schizophyllum commune</i> is a causative agent of allergic bronchopulmonary mycosis, allergic fungal rhinosinusitis, and basidiomycosis. Diagnosis of these diseases remains difficult because no commercially available tool exists to identify the pathogen. Unique volatile organic compounds produced by a pathogen might be useful for non-invasive diagnosis. Here, we explored microbial volatile organic compounds produced by <i>S. commune</i>. Volatile sulfur compounds, dimethyl disulfide (48 of 49 strains) and methyl ethyl disulfide (49 of 49 strains), diethyl disulfide (34 of 49 strains), dimethyl trisulfide (40 of 49 strains), and dimethyl tetrasulfide (32 of 49 strains) were detected from headspace air in <i>S. commune</i> cultured vials. Every <i>S. commune</i> strain produced at least one volatile sulfur compound analyzed in this study. Those volatile sulfur compounds were not detected from the cultures of <i>Aspergillus</i> spp. (<i>A. fumigatus</i>, <i>A. flavus</i>, <i>A. niger</i>, and <i>A. terreus</i>), which are other major causative agents of allergic bronchopulmonary mycosis. The last, we examined H<sub>2</sub>S detection using lead acetate paper. Headspace air from <i>S. commune</i> rapidly turned the lead acetate paper black. These results suggest that those volatile sulfur compounds are potent targets for the diagnosis of <i>S. commune</i> and infectious diseases. |
| first_indexed | 2024-03-10T10:35:59Z |
| format | Article |
| id | doaj.art-3ec6b3ae07c74067809ddd839223c2ba |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-10T10:35:59Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-3ec6b3ae07c74067809ddd839223c2ba2023-11-21T23:19:29ZengMDPI AGJournal of Fungi2309-608X2021-06-017646510.3390/jof7060465Identification of Volatile Sulfur Compounds Produced by <i>Schizophyllum commune</i>Takahito Toyotome0Masahiko Takino1Masahiro Takaya2Maki Yahiro3Katsuhiko Kamei4Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, 2-1 Inada-cho Nishi, Obihiro, Hokkaido 080-8555, JapanJapan Application Center, Life Sciences and Chemical Analysis, Agilent Technologies Japan, Ltd., 9-1 Takakura-cho, Hachioji, Tokyo 192-8510, JapanThe Tokachi Foundation, Obihiro, Hokkaido 080-2462, JapanMedical Mycology Research Center, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba, Chiba 260-8673, JapanMedical Mycology Research Center, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba, Chiba 260-8673, Japan<i>Schizophyllum commune</i> is a causative agent of allergic bronchopulmonary mycosis, allergic fungal rhinosinusitis, and basidiomycosis. Diagnosis of these diseases remains difficult because no commercially available tool exists to identify the pathogen. Unique volatile organic compounds produced by a pathogen might be useful for non-invasive diagnosis. Here, we explored microbial volatile organic compounds produced by <i>S. commune</i>. Volatile sulfur compounds, dimethyl disulfide (48 of 49 strains) and methyl ethyl disulfide (49 of 49 strains), diethyl disulfide (34 of 49 strains), dimethyl trisulfide (40 of 49 strains), and dimethyl tetrasulfide (32 of 49 strains) were detected from headspace air in <i>S. commune</i> cultured vials. Every <i>S. commune</i> strain produced at least one volatile sulfur compound analyzed in this study. Those volatile sulfur compounds were not detected from the cultures of <i>Aspergillus</i> spp. (<i>A. fumigatus</i>, <i>A. flavus</i>, <i>A. niger</i>, and <i>A. terreus</i>), which are other major causative agents of allergic bronchopulmonary mycosis. The last, we examined H<sub>2</sub>S detection using lead acetate paper. Headspace air from <i>S. commune</i> rapidly turned the lead acetate paper black. These results suggest that those volatile sulfur compounds are potent targets for the diagnosis of <i>S. commune</i> and infectious diseases.https://www.mdpi.com/2309-608X/7/6/465allergic bronchopulmonary mycosismicrobial volatile organic compounds<i>Schizophyllum commune</i>volatile sulfur compounds |
| spellingShingle | Takahito Toyotome Masahiko Takino Masahiro Takaya Maki Yahiro Katsuhiko Kamei Identification of Volatile Sulfur Compounds Produced by <i>Schizophyllum commune</i> Journal of Fungi allergic bronchopulmonary mycosis microbial volatile organic compounds <i>Schizophyllum commune</i> volatile sulfur compounds |
| title | Identification of Volatile Sulfur Compounds Produced by <i>Schizophyllum commune</i> |
| title_full | Identification of Volatile Sulfur Compounds Produced by <i>Schizophyllum commune</i> |
| title_fullStr | Identification of Volatile Sulfur Compounds Produced by <i>Schizophyllum commune</i> |
| title_full_unstemmed | Identification of Volatile Sulfur Compounds Produced by <i>Schizophyllum commune</i> |
| title_short | Identification of Volatile Sulfur Compounds Produced by <i>Schizophyllum commune</i> |
| title_sort | identification of volatile sulfur compounds produced by i schizophyllum commune i |
| topic | allergic bronchopulmonary mycosis microbial volatile organic compounds <i>Schizophyllum commune</i> volatile sulfur compounds |
| url | https://www.mdpi.com/2309-608X/7/6/465 |
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