Recombinant expression and preliminary characterization of Peptidyl-prolyl cis/trans-isomerase Rrd1 from Saccharomyces cerevisiae.
Sacchromycescerevisiae Peptidyl-prolylcis/trans-isomerase Rrd1 has been linked to DNA repair, bud morphogenesis, advancement of the G1 phase, DNA replication stress, microtubule dynamics and is also necessary for the quick decrease in Sgs1p levels in response to rapamycin. In present study, Rrd1 gen...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2023-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0282749 |
| _version_ | 1797801962093150208 |
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| author | Mohd Kashif Ahad Amer Alsaiari Bhupendra Kumar Mohd Asalam Mohammad Imran Khan Abrar Ahmad Rayees Ahmad Lone Mazen Almehmadi Mazin A Zamzami Mohd Sohail Akhtar |
| author_facet | Mohd Kashif Ahad Amer Alsaiari Bhupendra Kumar Mohd Asalam Mohammad Imran Khan Abrar Ahmad Rayees Ahmad Lone Mazen Almehmadi Mazin A Zamzami Mohd Sohail Akhtar |
| author_sort | Mohd Kashif |
| collection | DOAJ |
| description | Sacchromycescerevisiae Peptidyl-prolylcis/trans-isomerase Rrd1 has been linked to DNA repair, bud morphogenesis, advancement of the G1 phase, DNA replication stress, microtubule dynamics and is also necessary for the quick decrease in Sgs1p levels in response to rapamycin. In present study, Rrd1 gene was amplified by standard PCR and subsequently cloned downstream to bacteriophage T7 inducible promoter and lac operator of expression vector pET21d(+). Additionally, immobilized metal affinity chromatography (IMAC) was used to purify the protein upto its homogeneity, and its homogeneous purity was further confirmed through western blotting. Size exclusion chromatography implies that Rrd1 is existing as monomer in its natural state. Foldwise Rrd1 protein belongs to PTPA-like protein superfamily. Rrd1 showed characteristic negative minima at 222 and 208 nm represent protein typically acquired α helix in the far-UV CD spectra. Fluorescence spectra showed properly folded tertiary structures of Rrd1 at physiological conditions. Rrd1protein can be identified from different species using a fingerprint created by PIPSA analysis. The protein's abundance could aid in its crystallization, biophysical characterization and identification of other-interacting partners of Rrd1 protein. |
| first_indexed | 2024-03-13T04:58:31Z |
| format | Article |
| id | doaj.art-3ecacdce2c07457db9f93cd7744637f5 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-03-13T04:58:31Z |
| publishDate | 2023-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-3ecacdce2c07457db9f93cd7744637f52023-06-17T05:31:47ZengPublic Library of Science (PLoS)PLoS ONE1932-62032023-01-01186e028274910.1371/journal.pone.0282749Recombinant expression and preliminary characterization of Peptidyl-prolyl cis/trans-isomerase Rrd1 from Saccharomyces cerevisiae.Mohd KashifAhad Amer AlsaiariBhupendra KumarMohd AsalamMohammad Imran KhanAbrar AhmadRayees Ahmad LoneMazen AlmehmadiMazin A ZamzamiMohd Sohail AkhtarSacchromycescerevisiae Peptidyl-prolylcis/trans-isomerase Rrd1 has been linked to DNA repair, bud morphogenesis, advancement of the G1 phase, DNA replication stress, microtubule dynamics and is also necessary for the quick decrease in Sgs1p levels in response to rapamycin. In present study, Rrd1 gene was amplified by standard PCR and subsequently cloned downstream to bacteriophage T7 inducible promoter and lac operator of expression vector pET21d(+). Additionally, immobilized metal affinity chromatography (IMAC) was used to purify the protein upto its homogeneity, and its homogeneous purity was further confirmed through western blotting. Size exclusion chromatography implies that Rrd1 is existing as monomer in its natural state. Foldwise Rrd1 protein belongs to PTPA-like protein superfamily. Rrd1 showed characteristic negative minima at 222 and 208 nm represent protein typically acquired α helix in the far-UV CD spectra. Fluorescence spectra showed properly folded tertiary structures of Rrd1 at physiological conditions. Rrd1protein can be identified from different species using a fingerprint created by PIPSA analysis. The protein's abundance could aid in its crystallization, biophysical characterization and identification of other-interacting partners of Rrd1 protein.https://doi.org/10.1371/journal.pone.0282749 |
| spellingShingle | Mohd Kashif Ahad Amer Alsaiari Bhupendra Kumar Mohd Asalam Mohammad Imran Khan Abrar Ahmad Rayees Ahmad Lone Mazen Almehmadi Mazin A Zamzami Mohd Sohail Akhtar Recombinant expression and preliminary characterization of Peptidyl-prolyl cis/trans-isomerase Rrd1 from Saccharomyces cerevisiae. PLoS ONE |
| title | Recombinant expression and preliminary characterization of Peptidyl-prolyl cis/trans-isomerase Rrd1 from Saccharomyces cerevisiae. |
| title_full | Recombinant expression and preliminary characterization of Peptidyl-prolyl cis/trans-isomerase Rrd1 from Saccharomyces cerevisiae. |
| title_fullStr | Recombinant expression and preliminary characterization of Peptidyl-prolyl cis/trans-isomerase Rrd1 from Saccharomyces cerevisiae. |
| title_full_unstemmed | Recombinant expression and preliminary characterization of Peptidyl-prolyl cis/trans-isomerase Rrd1 from Saccharomyces cerevisiae. |
| title_short | Recombinant expression and preliminary characterization of Peptidyl-prolyl cis/trans-isomerase Rrd1 from Saccharomyces cerevisiae. |
| title_sort | recombinant expression and preliminary characterization of peptidyl prolyl cis trans isomerase rrd1 from saccharomyces cerevisiae |
| url | https://doi.org/10.1371/journal.pone.0282749 |
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