Muscle Cell Morphogenesis, Structure, Development and Differentiation Processes Are Significantly Regulated during Human Ovarian Granulosa Cells In Vitro Cultivation
Granulosa cells (GCs) have many functions and are fundamental for both folliculogenesis and oogenesis, releasing hormones and communicating directly with the oocyte. Long-term in vitro cultures of GCs show significant stem-like characteristics. In the current study, RNA of human ovarian granulosa ce...
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MDPI AG
2020-06-01
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/9/6/2006 |
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| author | Claudia Dompe Wiesława Kranc Karol Jopek Katarzyna Kowalska Sylwia Ciesiółka Błażej Chermuła Artur Bryja Maurycy Jankowski Joanna Perek Małgorzata Józkowiak Lisa Moncrieff Greg Hutchings Krzysztof Janowicz Leszek Pawelczyk Małgorzata Bruska James Petitte Paul Mozdziak Magdalena Kulus Hanna Piotrowska-Kempisty Robert Z. Spaczyński Michał Nowicki Bartosz Kempisty |
| author_facet | Claudia Dompe Wiesława Kranc Karol Jopek Katarzyna Kowalska Sylwia Ciesiółka Błażej Chermuła Artur Bryja Maurycy Jankowski Joanna Perek Małgorzata Józkowiak Lisa Moncrieff Greg Hutchings Krzysztof Janowicz Leszek Pawelczyk Małgorzata Bruska James Petitte Paul Mozdziak Magdalena Kulus Hanna Piotrowska-Kempisty Robert Z. Spaczyński Michał Nowicki Bartosz Kempisty |
| author_sort | Claudia Dompe |
| collection | DOAJ |
| description | Granulosa cells (GCs) have many functions and are fundamental for both folliculogenesis and oogenesis, releasing hormones and communicating directly with the oocyte. Long-term in vitro cultures of GCs show significant stem-like characteristics. In the current study, RNA of human ovarian granulosa cells was collected at 1, 7, 15 and 30 days of long-term in vitro culture. Understanding the process of differentiation of GCs towards different cell lineages, as well as the molecular pathways underlying these mechanisms, is fundamental to revealing other possible stemness markers of this type of cell. Identifying new markers of GC plasticity may help to understand the aetiology and recurrence of a wide variety of diseases and health conditions and reveal possible clinical applications of the ovarian tissue cells, affecting not only the reproductive ability but also sex hormone production. Granulosa cells were the subject of this study, as they are readily available as remnant material leftover after in vitro fertilisation procedures and exhibit significant stem-like characteristics in culture. The change in gene expression was investigated through a range of molecular and bioinformatic analyses. Expression microarrays were used, allowing the identification of groups of genes typical of specific cellular pathways. This candidate gene study focused on ontological groups associated with muscle cell morphogenesis, structure, development and differentiation, namely, “muscle cell development”, “muscle cell differentiation”, “muscle contraction”, “muscle organ development”, “muscle organ morphogenesis”, “muscle structure development”, “muscle system process” and “muscle tissue development”. The results showed that the 10 most upregulated genes were keratin 19, oxytocin receptor, connective tissue growth factor, nexilin, myosin light chain kinase, cysteine and glycine-rich protein 3, caveolin 1, actin, activating transcription factor 3 and tropomyosin, while the 10 most downregulated consisted of epiregulin, prostaglandin-endoperoxide synthase 2, transforming growth factor, interleukin, collagen, 5-hydroxytryptmine, interleukin 4, phosphodiesterase, wingless-type MMTV integration site family and SRY-box 9. Moreover, ultrastructural observations showing heterogeneity of granulosa cell population are presented in the study. At least two morphologically different subpopulations were identified: large, light coloured and small, darker cells. The expression of genes belonging to the mentioned ontological groups suggest the potential ability of GCs to differentiate and proliferate toward muscle lineage, showing possible application in muscle regeneration and the treatment of different diseases. |
| first_indexed | 2024-03-10T18:52:10Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-10T18:52:10Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Clinical Medicine |
| spelling | doaj.art-3ecacfba0e9f400bb20376e92f3aaa532023-11-20T05:02:10ZengMDPI AGJournal of Clinical Medicine2077-03832020-06-0196200610.3390/jcm9062006Muscle Cell Morphogenesis, Structure, Development and Differentiation Processes Are Significantly Regulated during Human Ovarian Granulosa Cells In Vitro CultivationClaudia Dompe0Wiesława Kranc1Karol Jopek2Katarzyna Kowalska3Sylwia Ciesiółka4Błażej Chermuła5Artur Bryja6Maurycy Jankowski7Joanna Perek8Małgorzata Józkowiak9Lisa Moncrieff10Greg Hutchings11Krzysztof Janowicz12Leszek Pawelczyk13Małgorzata Bruska14James Petitte15Paul Mozdziak16Magdalena Kulus17Hanna Piotrowska-Kempisty18Robert Z. Spaczyński19Michał Nowicki20Bartosz Kempisty21The School of Medicine, Medical Sciences and Nutrition, University of Aberdeen, Aberdeen AB25 2ZD, UKDepartment of Anatomy, Poznan University of Medical Sciences, 6 Święcickiego St., 60-781 Poznan, PolandDepartment of Histology and Embryology, Poznan University of Medical Sciences, 6 Święcickiego St., 60-781 Poznan, PolandDepartment of Histology and Embryology, Poznan University of Medical Sciences, 6 Święcickiego St., 60-781 Poznan, PolandDepartment of Histology and Embryology, Poznan University of Medical Sciences, 6 Święcickiego St., 60-781 Poznan, PolandDivision of Infertility and Reproductive Endocrinology, Department of Gynecology, Obstetrics and Gynecological Oncology, Poznan University of Medical Sciences, 33 Polna St., 60-535 Poznan, PolandDepartment of Anatomy, Poznan University of Medical Sciences, 6 Święcickiego St., 60-781 Poznan, PolandDepartment of Anatomy, Poznan University of Medical Sciences, 6 Święcickiego St., 60-781 Poznan, PolandDepartment of Anatomy, Poznan University of Medical Sciences, 6 Święcickiego St., 60-781 Poznan, PolandDepartment of Toxicology, Poznan University of Medical Sciences, 30 Dojazd St., 60-631 Poznan, PolandThe School of Medicine, Medical Sciences and Nutrition, University of Aberdeen, Aberdeen AB25 2ZD, UKThe School of Medicine, Medical Sciences and Nutrition, University of Aberdeen, Aberdeen AB25 2ZD, UKThe School of Medicine, Medical Sciences and Nutrition, University of Aberdeen, Aberdeen AB25 2ZD, UKDivision of Infertility and Reproductive Endocrinology, Department of Gynecology, Obstetrics and Gynecological Oncology, Poznan University of Medical Sciences, 33 Polna St., 60-535 Poznan, PolandDepartment of Anatomy, Poznan University of Medical Sciences, 6 Święcickiego St., 60-781 Poznan, PolandPrestage Department of Poultry Science, North Carolina State University, Raleigh, NC 27695, USAPhysiology Graduate Program, North Carolina State University, Raleigh, NC 27695, USADepartment of Veterinary Surgery, Institute of Veterinary Medicine, Nicolaus Copernicus University in Torun, 1 Lwowska St., 87-100 Toruń, PolandDepartment of Toxicology, Poznan University of Medical Sciences, 30 Dojazd St., 60-631 Poznan, PolandDivision of Infertility and Reproductive Endocrinology, Department of Gynecology, Obstetrics and Gynecological Oncology, Poznan University of Medical Sciences, 33 Polna St., 60-535 Poznan, PolandDepartment of Histology and Embryology, Poznan University of Medical Sciences, 6 Święcickiego St., 60-781 Poznan, PolandDepartment of Histology and Embryology, Poznan University of Medical Sciences, 6 Święcickiego St., 60-781 Poznan, PolandGranulosa cells (GCs) have many functions and are fundamental for both folliculogenesis and oogenesis, releasing hormones and communicating directly with the oocyte. Long-term in vitro cultures of GCs show significant stem-like characteristics. In the current study, RNA of human ovarian granulosa cells was collected at 1, 7, 15 and 30 days of long-term in vitro culture. Understanding the process of differentiation of GCs towards different cell lineages, as well as the molecular pathways underlying these mechanisms, is fundamental to revealing other possible stemness markers of this type of cell. Identifying new markers of GC plasticity may help to understand the aetiology and recurrence of a wide variety of diseases and health conditions and reveal possible clinical applications of the ovarian tissue cells, affecting not only the reproductive ability but also sex hormone production. Granulosa cells were the subject of this study, as they are readily available as remnant material leftover after in vitro fertilisation procedures and exhibit significant stem-like characteristics in culture. The change in gene expression was investigated through a range of molecular and bioinformatic analyses. Expression microarrays were used, allowing the identification of groups of genes typical of specific cellular pathways. This candidate gene study focused on ontological groups associated with muscle cell morphogenesis, structure, development and differentiation, namely, “muscle cell development”, “muscle cell differentiation”, “muscle contraction”, “muscle organ development”, “muscle organ morphogenesis”, “muscle structure development”, “muscle system process” and “muscle tissue development”. The results showed that the 10 most upregulated genes were keratin 19, oxytocin receptor, connective tissue growth factor, nexilin, myosin light chain kinase, cysteine and glycine-rich protein 3, caveolin 1, actin, activating transcription factor 3 and tropomyosin, while the 10 most downregulated consisted of epiregulin, prostaglandin-endoperoxide synthase 2, transforming growth factor, interleukin, collagen, 5-hydroxytryptmine, interleukin 4, phosphodiesterase, wingless-type MMTV integration site family and SRY-box 9. Moreover, ultrastructural observations showing heterogeneity of granulosa cell population are presented in the study. At least two morphologically different subpopulations were identified: large, light coloured and small, darker cells. The expression of genes belonging to the mentioned ontological groups suggest the potential ability of GCs to differentiate and proliferate toward muscle lineage, showing possible application in muscle regeneration and the treatment of different diseases.https://www.mdpi.com/2077-0383/9/6/2006human GCsin vitro cultureproliferationmuscle differentiation |
| spellingShingle | Claudia Dompe Wiesława Kranc Karol Jopek Katarzyna Kowalska Sylwia Ciesiółka Błażej Chermuła Artur Bryja Maurycy Jankowski Joanna Perek Małgorzata Józkowiak Lisa Moncrieff Greg Hutchings Krzysztof Janowicz Leszek Pawelczyk Małgorzata Bruska James Petitte Paul Mozdziak Magdalena Kulus Hanna Piotrowska-Kempisty Robert Z. Spaczyński Michał Nowicki Bartosz Kempisty Muscle Cell Morphogenesis, Structure, Development and Differentiation Processes Are Significantly Regulated during Human Ovarian Granulosa Cells In Vitro Cultivation Journal of Clinical Medicine human GCs in vitro culture proliferation muscle differentiation |
| title | Muscle Cell Morphogenesis, Structure, Development and Differentiation Processes Are Significantly Regulated during Human Ovarian Granulosa Cells In Vitro Cultivation |
| title_full | Muscle Cell Morphogenesis, Structure, Development and Differentiation Processes Are Significantly Regulated during Human Ovarian Granulosa Cells In Vitro Cultivation |
| title_fullStr | Muscle Cell Morphogenesis, Structure, Development and Differentiation Processes Are Significantly Regulated during Human Ovarian Granulosa Cells In Vitro Cultivation |
| title_full_unstemmed | Muscle Cell Morphogenesis, Structure, Development and Differentiation Processes Are Significantly Regulated during Human Ovarian Granulosa Cells In Vitro Cultivation |
| title_short | Muscle Cell Morphogenesis, Structure, Development and Differentiation Processes Are Significantly Regulated during Human Ovarian Granulosa Cells In Vitro Cultivation |
| title_sort | muscle cell morphogenesis structure development and differentiation processes are significantly regulated during human ovarian granulosa cells in vitro cultivation |
| topic | human GCs in vitro culture proliferation muscle differentiation |
| url | https://www.mdpi.com/2077-0383/9/6/2006 |
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