Early Lipid Raft-Related Changes: Interplay between Unilateral Denervation and Hindlimb Suspension
Muscle disuse and denervation leads to muscle atrophy, but underlying mechanisms can be different. Previously, we have found ceramide (Cer) accumulation and lipid raft disruption after acute hindlimb suspension (HS), a model of muscle disuse. Herein, using biochemical and fluorescent approaches the...
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MDPI AG
2021-02-01
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| Series: | International Journal of Molecular Sciences |
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| author | Irina G. Bryndina Maria N. Shalagina Vladimir A. Protopopov Alexey V. Sekunov Andrey L. Zefirov Guzalia F. Zakirjanova Alexey M. Petrov |
| author_facet | Irina G. Bryndina Maria N. Shalagina Vladimir A. Protopopov Alexey V. Sekunov Andrey L. Zefirov Guzalia F. Zakirjanova Alexey M. Petrov |
| author_sort | Irina G. Bryndina |
| collection | DOAJ |
| description | Muscle disuse and denervation leads to muscle atrophy, but underlying mechanisms can be different. Previously, we have found ceramide (Cer) accumulation and lipid raft disruption after acute hindlimb suspension (HS), a model of muscle disuse. Herein, using biochemical and fluorescent approaches the influence of unilateral denervation itself and in combination with short-term HS on membrane-related parameters of rat soleus muscle was studied. Denervation increased immunoexpression of sphingomyelinase and Cer in plasmalemmal regions, but decreased Cer content in the raft fraction and enhanced lipid raft integrity. Preliminary denervation suppressed (1) HS-induced Cer accumulation in plasmalemmal regions, shown for both nonraft and raft-fractions; (2) HS-mediated decrease in lipid raft integrity. Similar to denervation, inhibition of the sciatic nerve afferents with capsaicin itself increased Cer plasmalemmal immunoexpression, but attenuated the membrane-related effects of HS. Finally, both denervation and capsaicin treatment increased immunoexpression of proapoptotic protein Bax and inhibited HS-driven increase in antiapoptotic protein Bcl-2. Thus, denervation can increase lipid raft formation and attenuate HS-induced alterations probably due to decrease of Cer levels in the raft fraction. The effects of denervation could be at least partially caused by the loss of afferentation. The study points to the importance of motor and afferent inputs in control of Cer distribution and thereby stability of lipid rafts in the junctional and extrajunctional membranes of the muscle. |
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| language | English |
| last_indexed | 2024-03-09T00:35:18Z |
| publishDate | 2021-02-01 |
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| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-3ecaef4e3c704860b435c9ba054a67f32023-12-11T18:12:15ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-02-01225223910.3390/ijms22052239Early Lipid Raft-Related Changes: Interplay between Unilateral Denervation and Hindlimb SuspensionIrina G. Bryndina0Maria N. Shalagina1Vladimir A. Protopopov2Alexey V. Sekunov3Andrey L. Zefirov4Guzalia F. Zakirjanova5Alexey M. Petrov6Department of Pathophysiology and Immunology, Izhevsk State Medical Academy, Kommunarov St. 281, Izhevsk 426034, RussiaDepartment of Pathophysiology and Immunology, Izhevsk State Medical Academy, Kommunarov St. 281, Izhevsk 426034, RussiaDepartment of Pathophysiology and Immunology, Izhevsk State Medical Academy, Kommunarov St. 281, Izhevsk 426034, RussiaDepartment of Pathophysiology and Immunology, Izhevsk State Medical Academy, Kommunarov St. 281, Izhevsk 426034, RussiaInstitute of Neuroscience, Kazan State Medical University, Butlerova St. 49, Kazan 420012, RussiaInstitute of Neuroscience, Kazan State Medical University, Butlerova St. 49, Kazan 420012, RussiaInstitute of Neuroscience, Kazan State Medical University, Butlerova St. 49, Kazan 420012, RussiaMuscle disuse and denervation leads to muscle atrophy, but underlying mechanisms can be different. Previously, we have found ceramide (Cer) accumulation and lipid raft disruption after acute hindlimb suspension (HS), a model of muscle disuse. Herein, using biochemical and fluorescent approaches the influence of unilateral denervation itself and in combination with short-term HS on membrane-related parameters of rat soleus muscle was studied. Denervation increased immunoexpression of sphingomyelinase and Cer in plasmalemmal regions, but decreased Cer content in the raft fraction and enhanced lipid raft integrity. Preliminary denervation suppressed (1) HS-induced Cer accumulation in plasmalemmal regions, shown for both nonraft and raft-fractions; (2) HS-mediated decrease in lipid raft integrity. Similar to denervation, inhibition of the sciatic nerve afferents with capsaicin itself increased Cer plasmalemmal immunoexpression, but attenuated the membrane-related effects of HS. Finally, both denervation and capsaicin treatment increased immunoexpression of proapoptotic protein Bax and inhibited HS-driven increase in antiapoptotic protein Bcl-2. Thus, denervation can increase lipid raft formation and attenuate HS-induced alterations probably due to decrease of Cer levels in the raft fraction. The effects of denervation could be at least partially caused by the loss of afferentation. The study points to the importance of motor and afferent inputs in control of Cer distribution and thereby stability of lipid rafts in the junctional and extrajunctional membranes of the muscle.https://www.mdpi.com/1422-0067/22/5/2239skeletal muscledisusedenervationdeafferentationcapsaicinlipid rafts |
| spellingShingle | Irina G. Bryndina Maria N. Shalagina Vladimir A. Protopopov Alexey V. Sekunov Andrey L. Zefirov Guzalia F. Zakirjanova Alexey M. Petrov Early Lipid Raft-Related Changes: Interplay between Unilateral Denervation and Hindlimb Suspension International Journal of Molecular Sciences skeletal muscle disuse denervation deafferentation capsaicin lipid rafts |
| title | Early Lipid Raft-Related Changes: Interplay between Unilateral Denervation and Hindlimb Suspension |
| title_full | Early Lipid Raft-Related Changes: Interplay between Unilateral Denervation and Hindlimb Suspension |
| title_fullStr | Early Lipid Raft-Related Changes: Interplay between Unilateral Denervation and Hindlimb Suspension |
| title_full_unstemmed | Early Lipid Raft-Related Changes: Interplay between Unilateral Denervation and Hindlimb Suspension |
| title_short | Early Lipid Raft-Related Changes: Interplay between Unilateral Denervation and Hindlimb Suspension |
| title_sort | early lipid raft related changes interplay between unilateral denervation and hindlimb suspension |
| topic | skeletal muscle disuse denervation deafferentation capsaicin lipid rafts |
| url | https://www.mdpi.com/1422-0067/22/5/2239 |
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