Potential urine proteomics biomarkers for primary nephrotic syndrome
Abstract Background Nephrotic syndrome (NS) is a nonspecific kidney disorder, commonly caused by minimal change disease (MCD), focal segmental glomerulosclerosis (FSGS), and membranous nephropathy (MN). Here we analyzed urinary protein profiles, aiming to discover disease-specific biomarkers of thes...
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| Format: | Article |
| Language: | English |
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BMC
2017-05-01
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| Series: | Clinical Proteomics |
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| Online Access: | http://link.springer.com/article/10.1186/s12014-017-9153-1 |
| _version_ | 1818323735802806272 |
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| author | Young Wook Choi Yang Gyun Kim Min-Young Song Ju-Young Moon Kyung-Hwan Jeong Tae-Won Lee Chun-Gyoo Ihm Kang-Sik Park Sang-Ho Lee |
| author_facet | Young Wook Choi Yang Gyun Kim Min-Young Song Ju-Young Moon Kyung-Hwan Jeong Tae-Won Lee Chun-Gyoo Ihm Kang-Sik Park Sang-Ho Lee |
| author_sort | Young Wook Choi |
| collection | DOAJ |
| description | Abstract Background Nephrotic syndrome (NS) is a nonspecific kidney disorder, commonly caused by minimal change disease (MCD), focal segmental glomerulosclerosis (FSGS), and membranous nephropathy (MN). Here we analyzed urinary protein profiles, aiming to discover disease-specific biomarkers of these three common diseases in NS. Methods Sixteen urine samples were collected from patients with biopsy-proven NS and healthy controls. After removal of high-abundance proteins, the urinary protein profile was analyzed by LC–MS/MS to generate a discovery set. For validation, ELISA was used to analyze the selected proteins in 61 urine samples. Results The discovery set included 228 urine proteins, of which 22 proteins were differently expressed in MCD, MN, and FSGS. Among these, C9, CD14, and SERPINA1 were validated by ELISA. All three proteins were elevated in MCD, MN, and FSGS groups compared with in IgA nephropathy and healthy controls. When a regression model was applied, receiver operating characteristic analysis clearly discriminated MCD from the other causative diseases in NS. Conclusions We developed a disease-specific protein panel that discriminated between three main causes of NS. Through this pilot study, we suggest that urine proteomics could be a non-invasive and clinically available tool to discriminate MCD from MN and FSGS. |
| first_indexed | 2024-12-13T11:17:25Z |
| format | Article |
| id | doaj.art-3ecba2cb95d5497c954bfa9d0276ec0e |
| institution | Directory Open Access Journal |
| issn | 1542-6416 1559-0275 |
| language | English |
| last_indexed | 2024-12-13T11:17:25Z |
| publishDate | 2017-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Clinical Proteomics |
| spelling | doaj.art-3ecba2cb95d5497c954bfa9d0276ec0e2022-12-21T23:48:35ZengBMCClinical Proteomics1542-64161559-02752017-05-011411910.1186/s12014-017-9153-1Potential urine proteomics biomarkers for primary nephrotic syndromeYoung Wook Choi0Yang Gyun Kim1Min-Young Song2Ju-Young Moon3Kyung-Hwan Jeong4Tae-Won Lee5Chun-Gyoo Ihm6Kang-Sik Park7Sang-Ho Lee8Division of Nephrology, Department of Internal Medicine, Kyung Hee University School of MedicineDivision of Nephrology, Department of Internal Medicine, Kyung Hee University School of MedicineDepartment of Physiology, Kyung Hee University School of MedicineDivision of Nephrology, Department of Internal Medicine, Kyung Hee University School of MedicineDivision of Nephrology, Department of Internal Medicine, Kyung Hee University School of MedicineDivision of Nephrology, Department of Internal Medicine, Kyung Hee University School of MedicineDivision of Nephrology, Department of Internal Medicine, Kyung Hee University School of MedicineDepartment of Physiology, Kyung Hee University School of MedicineDivision of Nephrology, Department of Internal Medicine, Kyung Hee University School of MedicineAbstract Background Nephrotic syndrome (NS) is a nonspecific kidney disorder, commonly caused by minimal change disease (MCD), focal segmental glomerulosclerosis (FSGS), and membranous nephropathy (MN). Here we analyzed urinary protein profiles, aiming to discover disease-specific biomarkers of these three common diseases in NS. Methods Sixteen urine samples were collected from patients with biopsy-proven NS and healthy controls. After removal of high-abundance proteins, the urinary protein profile was analyzed by LC–MS/MS to generate a discovery set. For validation, ELISA was used to analyze the selected proteins in 61 urine samples. Results The discovery set included 228 urine proteins, of which 22 proteins were differently expressed in MCD, MN, and FSGS. Among these, C9, CD14, and SERPINA1 were validated by ELISA. All three proteins were elevated in MCD, MN, and FSGS groups compared with in IgA nephropathy and healthy controls. When a regression model was applied, receiver operating characteristic analysis clearly discriminated MCD from the other causative diseases in NS. Conclusions We developed a disease-specific protein panel that discriminated between three main causes of NS. Through this pilot study, we suggest that urine proteomics could be a non-invasive and clinically available tool to discriminate MCD from MN and FSGS.http://link.springer.com/article/10.1186/s12014-017-9153-1Focal and segmental glomerulosclerosisMinimal change diseaseMembranous nephropathyNephrotic syndromeUrine proteomics |
| spellingShingle | Young Wook Choi Yang Gyun Kim Min-Young Song Ju-Young Moon Kyung-Hwan Jeong Tae-Won Lee Chun-Gyoo Ihm Kang-Sik Park Sang-Ho Lee Potential urine proteomics biomarkers for primary nephrotic syndrome Clinical Proteomics Focal and segmental glomerulosclerosis Minimal change disease Membranous nephropathy Nephrotic syndrome Urine proteomics |
| title | Potential urine proteomics biomarkers for primary nephrotic syndrome |
| title_full | Potential urine proteomics biomarkers for primary nephrotic syndrome |
| title_fullStr | Potential urine proteomics biomarkers for primary nephrotic syndrome |
| title_full_unstemmed | Potential urine proteomics biomarkers for primary nephrotic syndrome |
| title_short | Potential urine proteomics biomarkers for primary nephrotic syndrome |
| title_sort | potential urine proteomics biomarkers for primary nephrotic syndrome |
| topic | Focal and segmental glomerulosclerosis Minimal change disease Membranous nephropathy Nephrotic syndrome Urine proteomics |
| url | http://link.springer.com/article/10.1186/s12014-017-9153-1 |
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