Characteristic antimicrobial resistance of clinically isolated Stenotrophomonas maltophilia CYZ via complete genome sequence
Objectives: Stenotrophomonas maltophilia is an important multidrug-resistant pathogen that is associated with various serious nosocomial infections. In our study, we investigated the antimicrobial resistance traits of clinical S. maltophilia strain CYZ isolated from the sputum of an immunocompromise...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-12-01
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| Series: | Journal of Global Antimicrobial Resistance |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716520302393 |
| _version_ | 1818612343907549184 |
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| author | Jingtao Ma Junhua Feng Yue Shan Yan Zhao Hongxiu Qiao Lixin Xie Xiaoxuan Lin Changle Wang Xia Chuai |
| author_facet | Jingtao Ma Junhua Feng Yue Shan Yan Zhao Hongxiu Qiao Lixin Xie Xiaoxuan Lin Changle Wang Xia Chuai |
| author_sort | Jingtao Ma |
| collection | DOAJ |
| description | Objectives: Stenotrophomonas maltophilia is an important multidrug-resistant pathogen that is associated with various serious nosocomial infections. In our study, we investigated the antimicrobial resistance traits of clinical S. maltophilia strain CYZ isolated from the sputum of an immunocompromised patient. Methods: The whole genome sequence of S. maltophilia CYZ was investigated using a PacBio RS II system. The functions of all the predicted genes were annotated by the COG, GO and KEGG databases. Several types of antibiotics were selected to test the antimicrobial susceptibility, and a phylogenetic tree was constructed based on 16S rRNA gene sequence. Results: The genome of S. maltophilia CYZ has a length of 4,517,685 bp and contains 4077 predicted genes, with an average G + C content of 66.65%. Functional genomic analysis via the annotations of the COG and GO databases revealed that the isolate exhibited specific means to resist antibiotics. The annotated genes involved in flagella, pili or fimbriae, biofilm formation, polysaccharide and cyclic di-GMP may contribute to promote the ability of antimicrobial resistance. This strain showed susceptibility to levofloxacin, trimethoprim/sulfamethoxazole and minocycline according to antimicrobial susceptibility testing. The phylogenetic relationship indicated that S. maltophilia CYZ was closely related to S. maltophilia strains isolated from the nosocomial environment. Conclusions: The current results give a better understanding of the genetic characteristics of antimicrobial resistance in S. maltophilia CYZ and provide a genetic basis for further study of the phenotype. |
| first_indexed | 2024-12-16T15:44:43Z |
| format | Article |
| id | doaj.art-3eccdcf4452841a387367ef2b4c617a2 |
| institution | Directory Open Access Journal |
| issn | 2213-7165 |
| language | English |
| last_indexed | 2024-12-16T15:44:43Z |
| publishDate | 2020-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj.art-3eccdcf4452841a387367ef2b4c617a22022-12-21T22:25:50ZengElsevierJournal of Global Antimicrobial Resistance2213-71652020-12-0123186193Characteristic antimicrobial resistance of clinically isolated Stenotrophomonas maltophilia CYZ via complete genome sequenceJingtao Ma0Junhua Feng1Yue Shan2Yan Zhao3Hongxiu Qiao4Lixin Xie5Xiaoxuan Lin6Changle Wang7Xia Chuai8Department of Cardiology, The Fourth Hospital, Hebei Medical University, Shijiazhuang 050011, PR ChinaClinical Laboratory, The Fourth Hospital, Hebei Medical University, Shijiazhuang 050011, PR ChinaThe Fourth School of Clinical Medicine, Hebei Medical University, Shijiazhuang 050011, PR ChinaDepartment of Pathogenic Biology, Hebei Medical University, Shijiazhuang 050017, PR ChinaDepartment of Pathogenic Biology, Hebei Medical University, Shijiazhuang 050017, PR ChinaDepartment of Pathogenic Biology, Hebei Medical University, Shijiazhuang 050017, PR ChinaSchool of Pharmacy, Hebei Medical University, Shijiazhuang 050017, PR ChinaDepartment of Pathogenic Biology, Hebei Medical University, Shijiazhuang 050017, PR China; Corresponding authors.Department of Pathogenic Biology, Hebei Medical University, Shijiazhuang 050017, PR China; Corresponding authors.Objectives: Stenotrophomonas maltophilia is an important multidrug-resistant pathogen that is associated with various serious nosocomial infections. In our study, we investigated the antimicrobial resistance traits of clinical S. maltophilia strain CYZ isolated from the sputum of an immunocompromised patient. Methods: The whole genome sequence of S. maltophilia CYZ was investigated using a PacBio RS II system. The functions of all the predicted genes were annotated by the COG, GO and KEGG databases. Several types of antibiotics were selected to test the antimicrobial susceptibility, and a phylogenetic tree was constructed based on 16S rRNA gene sequence. Results: The genome of S. maltophilia CYZ has a length of 4,517,685 bp and contains 4077 predicted genes, with an average G + C content of 66.65%. Functional genomic analysis via the annotations of the COG and GO databases revealed that the isolate exhibited specific means to resist antibiotics. The annotated genes involved in flagella, pili or fimbriae, biofilm formation, polysaccharide and cyclic di-GMP may contribute to promote the ability of antimicrobial resistance. This strain showed susceptibility to levofloxacin, trimethoprim/sulfamethoxazole and minocycline according to antimicrobial susceptibility testing. The phylogenetic relationship indicated that S. maltophilia CYZ was closely related to S. maltophilia strains isolated from the nosocomial environment. Conclusions: The current results give a better understanding of the genetic characteristics of antimicrobial resistance in S. maltophilia CYZ and provide a genetic basis for further study of the phenotype.http://www.sciencedirect.com/science/article/pii/S2213716520302393Stenotrophomonas maltophiliaAntimicrobial resistanceBiofilm formationComplete genome sequenceBioinformatics analysis |
| spellingShingle | Jingtao Ma Junhua Feng Yue Shan Yan Zhao Hongxiu Qiao Lixin Xie Xiaoxuan Lin Changle Wang Xia Chuai Characteristic antimicrobial resistance of clinically isolated Stenotrophomonas maltophilia CYZ via complete genome sequence Journal of Global Antimicrobial Resistance Stenotrophomonas maltophilia Antimicrobial resistance Biofilm formation Complete genome sequence Bioinformatics analysis |
| title | Characteristic antimicrobial resistance of clinically isolated Stenotrophomonas maltophilia CYZ via complete genome sequence |
| title_full | Characteristic antimicrobial resistance of clinically isolated Stenotrophomonas maltophilia CYZ via complete genome sequence |
| title_fullStr | Characteristic antimicrobial resistance of clinically isolated Stenotrophomonas maltophilia CYZ via complete genome sequence |
| title_full_unstemmed | Characteristic antimicrobial resistance of clinically isolated Stenotrophomonas maltophilia CYZ via complete genome sequence |
| title_short | Characteristic antimicrobial resistance of clinically isolated Stenotrophomonas maltophilia CYZ via complete genome sequence |
| title_sort | characteristic antimicrobial resistance of clinically isolated stenotrophomonas maltophilia cyz via complete genome sequence |
| topic | Stenotrophomonas maltophilia Antimicrobial resistance Biofilm formation Complete genome sequence Bioinformatics analysis |
| url | http://www.sciencedirect.com/science/article/pii/S2213716520302393 |
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