Proteomic analysis of salt and osmotic-drought stress in alfalfa seedlings
Alfalfa is widely grown and is one of the most important forage crops in the world, but its growth and biomass production are markedly reduced under salt and drought stress, particularly during the early seedling stage. To identify the mechanisms behind salt and drought responsiveness at the alfalfa...
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| Format: | Article |
| Language: | English |
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Elsevier
2016-10-01
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| Series: | Journal of Integrative Agriculture |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311915612801 |
| _version_ | 1818596533835137024 |
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| author | Qiao-li MA Jun-mei KANG Rui-cai LONG Yan-jun CUI Tie-jun ZHANG Jun-bo XIONG Qing-chuan YANG Yan SUN |
| author_facet | Qiao-li MA Jun-mei KANG Rui-cai LONG Yan-jun CUI Tie-jun ZHANG Jun-bo XIONG Qing-chuan YANG Yan SUN |
| author_sort | Qiao-li MA |
| collection | DOAJ |
| description | Alfalfa is widely grown and is one of the most important forage crops in the world, but its growth and biomass production are markedly reduced under salt and drought stress, particularly during the early seedling stage. To identify the mechanisms behind salt and drought responsiveness at the alfalfa seedling stage, the proteins expressed were analyzed under no-treatment, 200 mol L−1 NaCl and 180 g L−1 PEG treatment conditions during the seedling stage. Out of more than 800 protein spots detected on two-dimensional electrophoresis (2-DE) gels, 35 proteins showed statistically significant responses (P<0.05) to NaCl and PEG stress, which were selected for tandem mass spectrometric identification, owing to their good resolution and abundance levels, and 32 proteins were positively identified. The identified proteins were divided into seven functional categories: photosynthetic metabolism, protein biosynthesis, folding and assembly, carbohydrate metabolism-associated proteins, stress defense related protein, metabolism of nucleic acid, other function categories and unknown proteins. Our results suggested that these proteins may play roles in alfalfa adaptation to salt and drought stress. Further study of these proteins will provide insights into the molecular mechanisms of abiotic stress and the discovery of new candidate markers in alfalfa. |
| first_indexed | 2024-12-16T11:33:26Z |
| format | Article |
| id | doaj.art-3ecfd7d673a2407cb7592ed291708ad7 |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-12-16T11:33:26Z |
| publishDate | 2016-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-3ecfd7d673a2407cb7592ed291708ad72022-12-21T22:33:11ZengElsevierJournal of Integrative Agriculture2095-31192016-10-01151022662278Proteomic analysis of salt and osmotic-drought stress in alfalfa seedlingsQiao-li MA0Jun-mei KANG1Rui-cai LONG2Yan-jun CUI3Tie-jun ZHANG4Jun-bo XIONG5Qing-chuan YANG6Yan SUN7College of Animal Science and Technology, China Agricultural University, Beijing 100193, P.R. China; School of Chemistry & Chemical Engineering, Ningxia University, Yinchuan 750021, P.R. China; MA Qiao-liInstitute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. China; KANG Jun-meiInstitute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. ChinaInstitute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. ChinaInstitute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. ChinaInstitute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. China; Institute of Animal Science, Hubei Academy of Agricultural Sciences, Wuhan 430064, P.R. ChinaInstitute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. ChinaCollege of Animal Science and Technology, China Agricultural University, Beijing 100193, P.R. China; Correspondence SUN Yan, Tel: +86-10-62732793Alfalfa is widely grown and is one of the most important forage crops in the world, but its growth and biomass production are markedly reduced under salt and drought stress, particularly during the early seedling stage. To identify the mechanisms behind salt and drought responsiveness at the alfalfa seedling stage, the proteins expressed were analyzed under no-treatment, 200 mol L−1 NaCl and 180 g L−1 PEG treatment conditions during the seedling stage. Out of more than 800 protein spots detected on two-dimensional electrophoresis (2-DE) gels, 35 proteins showed statistically significant responses (P<0.05) to NaCl and PEG stress, which were selected for tandem mass spectrometric identification, owing to their good resolution and abundance levels, and 32 proteins were positively identified. The identified proteins were divided into seven functional categories: photosynthetic metabolism, protein biosynthesis, folding and assembly, carbohydrate metabolism-associated proteins, stress defense related protein, metabolism of nucleic acid, other function categories and unknown proteins. Our results suggested that these proteins may play roles in alfalfa adaptation to salt and drought stress. Further study of these proteins will provide insights into the molecular mechanisms of abiotic stress and the discovery of new candidate markers in alfalfa.http://www.sciencedirect.com/science/article/pii/S2095311915612801alfalfasalinity stressosmotic stressseedling growthproteomics |
| spellingShingle | Qiao-li MA Jun-mei KANG Rui-cai LONG Yan-jun CUI Tie-jun ZHANG Jun-bo XIONG Qing-chuan YANG Yan SUN Proteomic analysis of salt and osmotic-drought stress in alfalfa seedlings Journal of Integrative Agriculture alfalfa salinity stress osmotic stress seedling growth proteomics |
| title | Proteomic analysis of salt and osmotic-drought stress in alfalfa seedlings |
| title_full | Proteomic analysis of salt and osmotic-drought stress in alfalfa seedlings |
| title_fullStr | Proteomic analysis of salt and osmotic-drought stress in alfalfa seedlings |
| title_full_unstemmed | Proteomic analysis of salt and osmotic-drought stress in alfalfa seedlings |
| title_short | Proteomic analysis of salt and osmotic-drought stress in alfalfa seedlings |
| title_sort | proteomic analysis of salt and osmotic drought stress in alfalfa seedlings |
| topic | alfalfa salinity stress osmotic stress seedling growth proteomics |
| url | http://www.sciencedirect.com/science/article/pii/S2095311915612801 |
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