Blastocystis spp. subtype 10 infected beef cattle in Kamal and Socah, Bangkalan, Madura, Indonesia
Background and Aim: Blastocystis spp. is a gastrointestinal parasite that can infect both humans and animals and has the potential to become a zoonotic parasite. This study analyzed a subtype (ST) of Blastocystis spp. that had infected beef cattle in Kamal and Socah, Bangkalan, Madura, Indonesia....
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Veterinary World
2020-02-01
|
| Series: | Veterinary World |
| Subjects: | |
| Online Access: | http://www.veterinaryworld.org/Vol.13/February-2020/2.pdf |
| _version_ | 1818923257788628992 |
|---|---|
| author | Lucia Tri Suwanti Yuli Susana Poedji Hastutiek Endang Suprihati Nunuk Dyah Retno Lastuti |
| author_facet | Lucia Tri Suwanti Yuli Susana Poedji Hastutiek Endang Suprihati Nunuk Dyah Retno Lastuti |
| author_sort | Lucia Tri Suwanti |
| collection | DOAJ |
| description | Background and Aim: Blastocystis spp. is a gastrointestinal parasite that can infect both humans and animals and has the potential to become a zoonotic parasite. This study analyzed a subtype (ST) of Blastocystis spp. that had infected beef cattle in Kamal and Socah, Bangkalan, Madura, Indonesia.
Materials and Methods: Fresh stool samples were collected from 108 beef cattle at Kamal and Socah, Bangkalan, Madura, Indonesia. Blastocystis spp. were detected both morphologically and genetically based on the 18S rRNA gene. The morphology of Blastocystis spp. from the stool samples and cultured samples were observed under a light microscope. Blastocystis spp. from 20 positive cultures were amplified through polymerase chain reaction, and the resultant sequences were identified by ST.
Results: One hundred and eight (100%) fecal samples from the fresh or cultured stools were positive morphologically for Blastocystis spp. Molecularly, all 20 of the samples selected for DNA analysis were found to be Blastocystis spp. ST 10.
Conclusion: Based on morphological and molecular detection, the prevalence of Blastocystis spp. infection in beef cattle within Kamal and Socah, Bangkalan, Madura, Indonesia, was high. About 100% were non-zoonotic parasites. This was the first report of Blastocystis spp. ST 10 found in infected beef cattle in Kamal and Socah, Bangkalan, Madura, Indonesia. |
| first_indexed | 2024-12-20T02:06:34Z |
| format | Article |
| id | doaj.art-3ed0010d0a804ba1b5425aeee2bdfb1c |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-12-20T02:06:34Z |
| publishDate | 2020-02-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-3ed0010d0a804ba1b5425aeee2bdfb1c2022-12-21T19:57:10ZengVeterinary WorldVeterinary World0972-89882231-09162020-02-0113223123710.14202/vetworld.2020.231-237Blastocystis spp. subtype 10 infected beef cattle in Kamal and Socah, Bangkalan, Madura, IndonesiaLucia Tri Suwanti0Yuli Susana1Poedji Hastutiek2Endang Suprihati3Nunuk Dyah Retno Lastuti4Department of Veterinary Parasitology, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Mulyorejo, Kampus C Unair, Surabaya, 60115, Indonesia; Toxoplasmosis Study Group, Institute of Tropical Diseases, Universitas Airlangga, Jl. Mulyorejo, Campus C Unair, Surabaya 60115, Indonesia.Magister Student, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Mulyorejo, Campus C Unair, Surabaya 60115, Indonesia.Department of Veterinary Parasitology, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Mulyorejo, Kampus C Unair, Surabaya, 60115, Indonesia.Department of Veterinary Parasitology, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Mulyorejo, Kampus C Unair, Surabaya, 60115, Indonesia.Department of Veterinary Parasitology, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Mulyorejo, Kampus C Unair, Surabaya, 60115, Indonesia.Background and Aim: Blastocystis spp. is a gastrointestinal parasite that can infect both humans and animals and has the potential to become a zoonotic parasite. This study analyzed a subtype (ST) of Blastocystis spp. that had infected beef cattle in Kamal and Socah, Bangkalan, Madura, Indonesia. Materials and Methods: Fresh stool samples were collected from 108 beef cattle at Kamal and Socah, Bangkalan, Madura, Indonesia. Blastocystis spp. were detected both morphologically and genetically based on the 18S rRNA gene. The morphology of Blastocystis spp. from the stool samples and cultured samples were observed under a light microscope. Blastocystis spp. from 20 positive cultures were amplified through polymerase chain reaction, and the resultant sequences were identified by ST. Results: One hundred and eight (100%) fecal samples from the fresh or cultured stools were positive morphologically for Blastocystis spp. Molecularly, all 20 of the samples selected for DNA analysis were found to be Blastocystis spp. ST 10. Conclusion: Based on morphological and molecular detection, the prevalence of Blastocystis spp. infection in beef cattle within Kamal and Socah, Bangkalan, Madura, Indonesia, was high. About 100% were non-zoonotic parasites. This was the first report of Blastocystis spp. ST 10 found in infected beef cattle in Kamal and Socah, Bangkalan, Madura, Indonesia.http://www.veterinaryworld.org/Vol.13/February-2020/2.pdfbeef cattleblastocystis spp. subtype 10maduraindonesiazoonotic |
| spellingShingle | Lucia Tri Suwanti Yuli Susana Poedji Hastutiek Endang Suprihati Nunuk Dyah Retno Lastuti Blastocystis spp. subtype 10 infected beef cattle in Kamal and Socah, Bangkalan, Madura, Indonesia Veterinary World beef cattle blastocystis spp. subtype 10 madura indonesia zoonotic |
| title | Blastocystis spp. subtype 10 infected beef cattle in Kamal and Socah, Bangkalan, Madura, Indonesia |
| title_full | Blastocystis spp. subtype 10 infected beef cattle in Kamal and Socah, Bangkalan, Madura, Indonesia |
| title_fullStr | Blastocystis spp. subtype 10 infected beef cattle in Kamal and Socah, Bangkalan, Madura, Indonesia |
| title_full_unstemmed | Blastocystis spp. subtype 10 infected beef cattle in Kamal and Socah, Bangkalan, Madura, Indonesia |
| title_short | Blastocystis spp. subtype 10 infected beef cattle in Kamal and Socah, Bangkalan, Madura, Indonesia |
| title_sort | blastocystis spp subtype 10 infected beef cattle in kamal and socah bangkalan madura indonesia |
| topic | beef cattle blastocystis spp. subtype 10 madura indonesia zoonotic |
| url | http://www.veterinaryworld.org/Vol.13/February-2020/2.pdf |
| work_keys_str_mv | AT luciatrisuwanti blastocystissppsubtype10infectedbeefcattleinkamalandsocahbangkalanmaduraindonesia AT yulisusana blastocystissppsubtype10infectedbeefcattleinkamalandsocahbangkalanmaduraindonesia AT poedjihastutiek blastocystissppsubtype10infectedbeefcattleinkamalandsocahbangkalanmaduraindonesia AT endangsuprihati blastocystissppsubtype10infectedbeefcattleinkamalandsocahbangkalanmaduraindonesia AT nunukdyahretnolastuti blastocystissppsubtype10infectedbeefcattleinkamalandsocahbangkalanmaduraindonesia |