Identification of Potential Host Plants of Sap-Sucking Insects (Hemiptera: Cicadellidae) Using Anchored Hybrid By-Catch Data
Reliable host plant records are available for only a small fraction of herbivorous insect species, despite their potential agricultural importance. Most available data on insect–plant associations have been obtained through field observations of occurrences of insects on particular plants. Molecular...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-10-01
|
| Series: | Insects |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-4450/12/11/964 |
| _version_ | 1797509917994647552 |
|---|---|
| author | Yanghui Cao Christopher H. Dietrich |
| author_facet | Yanghui Cao Christopher H. Dietrich |
| author_sort | Yanghui Cao |
| collection | DOAJ |
| description | Reliable host plant records are available for only a small fraction of herbivorous insect species, despite their potential agricultural importance. Most available data on insect–plant associations have been obtained through field observations of occurrences of insects on particular plants. Molecular methods have more recently been used to identify potential host plants using DNA extracted from insects, but most prior studies using these methods have focused on chewing insects that ingest tissues expected to contain large quantities of plant DNA. Screening of Illumina data obtained from sap feeders of the hemipteran family Cicadellidae (leafhoppers) using anchored hybrid enrichment indicates that, despite feeding on plant fluids, these insects often contain detectable quantities of plant DNA. Although inclusion of probes for bacterial <i>16S</i> in the original anchored hybrid probe kit yielded relatively high detection rates for chloroplast <i>16S</i>, the Illumina short reads also, in some cases, included DNA for various plant barcode genes as “by-catch”. Detection rates were generally only slightly higher for Typhlocybinae, which feed preferentially on parenchyma cell contents, compared to other groups of leafhoppers that feed preferentially on phloem or xylem. These results indicate that next-generation sequencing provides a powerful tool to investigate the specific association between individual insect and plant species. |
| first_indexed | 2024-03-10T05:24:32Z |
| format | Article |
| id | doaj.art-3ed04db8f8c746e0b81e1117ef0e75e8 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T05:24:32Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-3ed04db8f8c746e0b81e1117ef0e75e82023-11-22T23:46:27ZengMDPI AGInsects2075-44502021-10-01121196410.3390/insects12110964Identification of Potential Host Plants of Sap-Sucking Insects (Hemiptera: Cicadellidae) Using Anchored Hybrid By-Catch DataYanghui Cao0Christopher H. Dietrich1Illinois Natural History Survey, Prairie Research Institute, University of Illinois, Champaign, IL 61820, USAIllinois Natural History Survey, Prairie Research Institute, University of Illinois, Champaign, IL 61820, USAReliable host plant records are available for only a small fraction of herbivorous insect species, despite their potential agricultural importance. Most available data on insect–plant associations have been obtained through field observations of occurrences of insects on particular plants. Molecular methods have more recently been used to identify potential host plants using DNA extracted from insects, but most prior studies using these methods have focused on chewing insects that ingest tissues expected to contain large quantities of plant DNA. Screening of Illumina data obtained from sap feeders of the hemipteran family Cicadellidae (leafhoppers) using anchored hybrid enrichment indicates that, despite feeding on plant fluids, these insects often contain detectable quantities of plant DNA. Although inclusion of probes for bacterial <i>16S</i> in the original anchored hybrid probe kit yielded relatively high detection rates for chloroplast <i>16S</i>, the Illumina short reads also, in some cases, included DNA for various plant barcode genes as “by-catch”. Detection rates were generally only slightly higher for Typhlocybinae, which feed preferentially on parenchyma cell contents, compared to other groups of leafhoppers that feed preferentially on phloem or xylem. These results indicate that next-generation sequencing provides a powerful tool to investigate the specific association between individual insect and plant species.https://www.mdpi.com/2075-4450/12/11/964leafhoppershost plantsinsect–plant associationanchored hybrid enrichment |
| spellingShingle | Yanghui Cao Christopher H. Dietrich Identification of Potential Host Plants of Sap-Sucking Insects (Hemiptera: Cicadellidae) Using Anchored Hybrid By-Catch Data Insects leafhoppers host plants insect–plant association anchored hybrid enrichment |
| title | Identification of Potential Host Plants of Sap-Sucking Insects (Hemiptera: Cicadellidae) Using Anchored Hybrid By-Catch Data |
| title_full | Identification of Potential Host Plants of Sap-Sucking Insects (Hemiptera: Cicadellidae) Using Anchored Hybrid By-Catch Data |
| title_fullStr | Identification of Potential Host Plants of Sap-Sucking Insects (Hemiptera: Cicadellidae) Using Anchored Hybrid By-Catch Data |
| title_full_unstemmed | Identification of Potential Host Plants of Sap-Sucking Insects (Hemiptera: Cicadellidae) Using Anchored Hybrid By-Catch Data |
| title_short | Identification of Potential Host Plants of Sap-Sucking Insects (Hemiptera: Cicadellidae) Using Anchored Hybrid By-Catch Data |
| title_sort | identification of potential host plants of sap sucking insects hemiptera cicadellidae using anchored hybrid by catch data |
| topic | leafhoppers host plants insect–plant association anchored hybrid enrichment |
| url | https://www.mdpi.com/2075-4450/12/11/964 |
| work_keys_str_mv | AT yanghuicao identificationofpotentialhostplantsofsapsuckinginsectshemipteracicadellidaeusinganchoredhybridbycatchdata AT christopherhdietrich identificationofpotentialhostplantsofsapsuckinginsectshemipteracicadellidaeusinganchoredhybridbycatchdata |