First Molecular Detection of Babesia gibsoni in Dogs from Wuhan, China
Canine piroplasmosis is a significant disease in dogs caused by Babesia and Theileria parasites. The clinical manifestations range from mild illness to serious disease depending on the parasite species and the physical condition of the infected dog. Canine piroplasmosis has been reported to be preva...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-08-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2017.01577/full |
| _version_ | 1818012094159650816 |
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| author | Lan He Lan He Xiaoyan Miao Xiaoyan Miao Jinfang Hu Jinfang Hu Yuan Huang Yuan Huang Pei He Pei He Junwei He Junwei He Long Yu Long Yu Ngabu Malobi Ngabu Malobi Ligang Shi Junlong Zhao Junlong Zhao |
| author_facet | Lan He Lan He Xiaoyan Miao Xiaoyan Miao Jinfang Hu Jinfang Hu Yuan Huang Yuan Huang Pei He Pei He Junwei He Junwei He Long Yu Long Yu Ngabu Malobi Ngabu Malobi Ligang Shi Junlong Zhao Junlong Zhao |
| author_sort | Lan He |
| collection | DOAJ |
| description | Canine piroplasmosis is a significant disease in dogs caused by Babesia and Theileria parasites. The clinical manifestations range from mild illness to serious disease depending on the parasite species and the physical condition of the infected dog. Canine piroplasmosis has been reported to be prevalent in China. However, no molecular evidence of the disease has been reported in pet dogs from Wuhan. In this study, 118 blood samples were randomly collected from pet dogs in veterinary clinics. The blood samples were subjected to both microscopic examination and reverse line blot (RLB) hybridization assays to detect piroplasm infection. Parasites were observed in 10 blood samples via microscopic examination, whereas there were 14 Babesia gibsoni-positive RLB tests. Phylogenetic analysis was performed after the 18S rRNA and ITS gene sequences from the 14 positive samples were cloned and sequenced. The results confirmed the existence of B. gibsoni in this area. This is the first molecular report of canine babesiosis in pet dogs from Wuhan, China. Pet dogs are companion animals, and the prevalence of babesiosis will be of concern in daily life. This study will help veterinarians better understand the prevalence of canine babesiosis and provide a guide for disease control in pet dogs. |
| first_indexed | 2024-04-14T06:15:41Z |
| format | Article |
| id | doaj.art-3ed07c6c277f423db53562d7b3578f0e |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-14T06:15:41Z |
| publishDate | 2017-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-3ed07c6c277f423db53562d7b3578f0e2022-12-22T02:08:12ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2017-08-01810.3389/fmicb.2017.01577279235First Molecular Detection of Babesia gibsoni in Dogs from Wuhan, ChinaLan He0Lan He1Xiaoyan Miao2Xiaoyan Miao3Jinfang Hu4Jinfang Hu5Yuan Huang6Yuan Huang7Pei He8Pei He9Junwei He10Junwei He11Long Yu12Long Yu13Ngabu Malobi14Ngabu Malobi15Ligang Shi16Junlong Zhao17Junlong Zhao18State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural UniversityWuhan, ChinaKey Laboratory for Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural UniversityWuhan, ChinaState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural UniversityWuhan, ChinaKey Laboratory for Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural UniversityWuhan, ChinaState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural UniversityWuhan, ChinaKey Laboratory for Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural UniversityWuhan, ChinaState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural UniversityWuhan, ChinaKey Laboratory for Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural UniversityWuhan, ChinaState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural UniversityWuhan, ChinaKey Laboratory for Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural UniversityWuhan, ChinaState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural UniversityWuhan, ChinaKey Laboratory for Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural UniversityWuhan, ChinaState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural UniversityWuhan, ChinaKey Laboratory for Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural UniversityWuhan, ChinaState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural UniversityWuhan, ChinaKey Laboratory for Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural UniversityWuhan, ChinaLuoyang Center for Animal Disease Control and PreventionLuoyang, ChinaState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural UniversityWuhan, ChinaKey Laboratory for Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural UniversityWuhan, ChinaCanine piroplasmosis is a significant disease in dogs caused by Babesia and Theileria parasites. The clinical manifestations range from mild illness to serious disease depending on the parasite species and the physical condition of the infected dog. Canine piroplasmosis has been reported to be prevalent in China. However, no molecular evidence of the disease has been reported in pet dogs from Wuhan. In this study, 118 blood samples were randomly collected from pet dogs in veterinary clinics. The blood samples were subjected to both microscopic examination and reverse line blot (RLB) hybridization assays to detect piroplasm infection. Parasites were observed in 10 blood samples via microscopic examination, whereas there were 14 Babesia gibsoni-positive RLB tests. Phylogenetic analysis was performed after the 18S rRNA and ITS gene sequences from the 14 positive samples were cloned and sequenced. The results confirmed the existence of B. gibsoni in this area. This is the first molecular report of canine babesiosis in pet dogs from Wuhan, China. Pet dogs are companion animals, and the prevalence of babesiosis will be of concern in daily life. This study will help veterinarians better understand the prevalence of canine babesiosis and provide a guide for disease control in pet dogs.http://journal.frontiersin.org/article/10.3389/fmicb.2017.01577/fullBabesia gibsonibabesiosisreverse line blot18S rRNApet dogcompanion animal |
| spellingShingle | Lan He Lan He Xiaoyan Miao Xiaoyan Miao Jinfang Hu Jinfang Hu Yuan Huang Yuan Huang Pei He Pei He Junwei He Junwei He Long Yu Long Yu Ngabu Malobi Ngabu Malobi Ligang Shi Junlong Zhao Junlong Zhao First Molecular Detection of Babesia gibsoni in Dogs from Wuhan, China Frontiers in Microbiology Babesia gibsoni babesiosis reverse line blot 18S rRNA pet dog companion animal |
| title | First Molecular Detection of Babesia gibsoni in Dogs from Wuhan, China |
| title_full | First Molecular Detection of Babesia gibsoni in Dogs from Wuhan, China |
| title_fullStr | First Molecular Detection of Babesia gibsoni in Dogs from Wuhan, China |
| title_full_unstemmed | First Molecular Detection of Babesia gibsoni in Dogs from Wuhan, China |
| title_short | First Molecular Detection of Babesia gibsoni in Dogs from Wuhan, China |
| title_sort | first molecular detection of babesia gibsoni in dogs from wuhan china |
| topic | Babesia gibsoni babesiosis reverse line blot 18S rRNA pet dog companion animal |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2017.01577/full |
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