Conventional and Molecular Cytogenetic Analyses in Turkish Patients with Multiple Myeloma
OBJECTIVE: Multiple myeloma (MM) is characterized by the accumulation and proliferation of malignant plasma cells, secreting monoclonal immunoglobulins and genetic abnormalities in MM have implications for disease progression and survival. In the present study, we investigated the frequency of chrom...
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| Format: | Article |
| Language: | English |
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Galenos Publishing House
2012-05-01
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| Series: | Turkish Journal of Hematology |
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| Online Access: | https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tjh&un=TJH-24993 |
| _version_ | 1797916902880706560 |
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| author | Beyhan Durak Aras Olga Meltem Akay Gülçin Sungar Güney Bademci Vahap Aslan Jülide Caferler Muhsin Özdemir Oğuz Çilingir Sevilhan Artan Zafer Gülbaş |
| author_facet | Beyhan Durak Aras Olga Meltem Akay Gülçin Sungar Güney Bademci Vahap Aslan Jülide Caferler Muhsin Özdemir Oğuz Çilingir Sevilhan Artan Zafer Gülbaş |
| author_sort | Beyhan Durak Aras |
| collection | DOAJ |
| description | OBJECTIVE: Multiple myeloma (MM) is characterized by the accumulation and proliferation of malignant plasma cells, secreting monoclonal immunoglobulins and genetic abnormalities in MM have implications for disease progression and survival. In the present study, we investigated the frequency of chromosomal abnormalities (CA) in Turkish patients with MM, using interphase FISH and CC and evaluated the relationship between the rearrangements detected, prognosis and stage of disease. METHODS: We performed conventional cytogenetic and FISH studies in 50 patients to detect chromosome anomalies associated with MM. FISH probes were used to detect 13q14, 13q34, 17p13 deletions, IGH rearrangements, and monosomy and/or trisomy of chromosomes 5, 9, and 15. RESULTS: CC studies could be performed in 32 of 50 cases and five patients (15.6%) showed chromosomal aberrations while 27 (84.3%) had normal karyotypes. By FISH, eighteen percent (9/50) of cases were found to be normal for all parameters evaluated. Eighty-two percent (41/50) of the patients were positive for at least one abnormality. Chromosome 13 anomalies were detected in 54% (27/50) of cases. The second most common aberration observed is chromosome 15 aberrations (50%). CONCLUSION: Median survival rate was shorter in patients with one of the abnormalities including chromosome 13 aberrations, IGH rearrangements or P53 deletions. Chromosome 15 aberrations were significantly higher in patients with stage III disease (p=0.02). We conclude that FISH studies should be performed in conjunction with conventional cytogenetic analysis for prognosis in multiple myeloma patients. |
| first_indexed | 2024-04-10T13:04:42Z |
| format | Article |
| id | doaj.art-3ed158a9a8f643929c7d05ec7f5a8a99 |
| institution | Directory Open Access Journal |
| issn | 1308-5263 |
| language | English |
| last_indexed | 2024-04-10T13:04:42Z |
| publishDate | 2012-05-01 |
| publisher | Galenos Publishing House |
| record_format | Article |
| series | Turkish Journal of Hematology |
| spelling | doaj.art-3ed158a9a8f643929c7d05ec7f5a8a992023-02-15T16:13:00ZengGalenos Publishing HouseTurkish Journal of Hematology1308-52632012-05-0129213514210.5152/tjh.2011.42TJH-24993Conventional and Molecular Cytogenetic Analyses in Turkish Patients with Multiple MyelomaBeyhan Durak Aras0Olga Meltem Akay1Gülçin Sungar2Güney Bademci3Vahap Aslan4Jülide Caferler5Muhsin Özdemir6Oğuz Çilingir7Sevilhan Artan8Zafer Gülbaş9Eskisehir Osmangazi University, Medical Faculty, Department Of Medical Genetics, Eskisehir, TurkeyEskisehir Osmangazi University, Medical Faculty, Department Of Hematology, Eskisehir, TurkeyEskisehir Osmangazi University, Medical Faculty, Department Of Medical Genetics, Eskisehir, TurkeyEskisehir Osmangazi University, Medical Faculty, Department Of Medical Genetics, Eskisehir, TurkeyYunus Emre State Hospital, Department Of Hematology, Eskisehir, TurkeyGenetiks Genetic Diagnosis Center, Istanbul, TurkeyEskisehir Osmangazi University, Medical Faculty, Department Of Medical Genetics, Eskisehir, TurkeyEskisehir Osmangazi University, Medical Faculty, Department Of Medical Genetics, Eskisehir, TurkeyEskisehir Osmangazi University, Medical Faculty, Department Of Medical Genetics, Eskisehir, TurkeyEskisehir Osmangazi University, Medical Faculty, Department Of Hematology, Eskisehir, TurkeyOBJECTIVE: Multiple myeloma (MM) is characterized by the accumulation and proliferation of malignant plasma cells, secreting monoclonal immunoglobulins and genetic abnormalities in MM have implications for disease progression and survival. In the present study, we investigated the frequency of chromosomal abnormalities (CA) in Turkish patients with MM, using interphase FISH and CC and evaluated the relationship between the rearrangements detected, prognosis and stage of disease. METHODS: We performed conventional cytogenetic and FISH studies in 50 patients to detect chromosome anomalies associated with MM. FISH probes were used to detect 13q14, 13q34, 17p13 deletions, IGH rearrangements, and monosomy and/or trisomy of chromosomes 5, 9, and 15. RESULTS: CC studies could be performed in 32 of 50 cases and five patients (15.6%) showed chromosomal aberrations while 27 (84.3%) had normal karyotypes. By FISH, eighteen percent (9/50) of cases were found to be normal for all parameters evaluated. Eighty-two percent (41/50) of the patients were positive for at least one abnormality. Chromosome 13 anomalies were detected in 54% (27/50) of cases. The second most common aberration observed is chromosome 15 aberrations (50%). CONCLUSION: Median survival rate was shorter in patients with one of the abnormalities including chromosome 13 aberrations, IGH rearrangements or P53 deletions. Chromosome 15 aberrations were significantly higher in patients with stage III disease (p=0.02). We conclude that FISH studies should be performed in conjunction with conventional cytogenetic analysis for prognosis in multiple myeloma patients.https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tjh&un=TJH-24993cytogenetcismyelomafishchromosomal aberrations |
| spellingShingle | Beyhan Durak Aras Olga Meltem Akay Gülçin Sungar Güney Bademci Vahap Aslan Jülide Caferler Muhsin Özdemir Oğuz Çilingir Sevilhan Artan Zafer Gülbaş Conventional and Molecular Cytogenetic Analyses in Turkish Patients with Multiple Myeloma Turkish Journal of Hematology cytogenetcis myeloma fish chromosomal aberrations |
| title | Conventional and Molecular Cytogenetic Analyses in Turkish Patients with Multiple Myeloma |
| title_full | Conventional and Molecular Cytogenetic Analyses in Turkish Patients with Multiple Myeloma |
| title_fullStr | Conventional and Molecular Cytogenetic Analyses in Turkish Patients with Multiple Myeloma |
| title_full_unstemmed | Conventional and Molecular Cytogenetic Analyses in Turkish Patients with Multiple Myeloma |
| title_short | Conventional and Molecular Cytogenetic Analyses in Turkish Patients with Multiple Myeloma |
| title_sort | conventional and molecular cytogenetic analyses in turkish patients with multiple myeloma |
| topic | cytogenetcis myeloma fish chromosomal aberrations |
| url | https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tjh&un=TJH-24993 |
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