Comprehensive Transcriptional Profiling and Mouse Phenotyping Reveals Dispensable Role for Adipose Tissue Selective Long Noncoding RNA <i>Gm15551</i>
Cold and nutrient-activated brown adipose tissue (BAT) is capable of increasing systemic energy expenditure via the uncoupled respiration and secretion of endocrine factors, thereby protecting mice against diet-induced obesity and improving insulin response and glucose tolerance in men. Long non-cod...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-05-01
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| Series: | Non-Coding RNA |
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| Online Access: | https://www.mdpi.com/2311-553X/8/3/32 |
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| author | Christoph Andreas Engelhard Chien Huang Sajjad Khani Petr Kasparek Jan Prochazka Jan Rozman David Pajuelo Reguera Radislav Sedlacek Jan-Wilhelm Kornfeld |
| author_facet | Christoph Andreas Engelhard Chien Huang Sajjad Khani Petr Kasparek Jan Prochazka Jan Rozman David Pajuelo Reguera Radislav Sedlacek Jan-Wilhelm Kornfeld |
| author_sort | Christoph Andreas Engelhard |
| collection | DOAJ |
| description | Cold and nutrient-activated brown adipose tissue (BAT) is capable of increasing systemic energy expenditure via the uncoupled respiration and secretion of endocrine factors, thereby protecting mice against diet-induced obesity and improving insulin response and glucose tolerance in men. Long non-coding RNAs (lncRNAs) have recently been identified as fine-tuning regulators of cellular function. While certain lncRNAs have been functionally characterised in adipose tissue, their overall contribution in the activation of BAT remains elusive. We identified lncRNAs correlating to interscapular brown adipose tissue (iBAT) function in a high fat diet (HFD) and cold stressed mice. We focused on <i>Gm15551</i>, which has an adipose tissue specific expression profile, is highly upregulated during adipogenesis, and downregulated by β-adrenergic activation in mature adipocytes. Although we performed comprehensive transcriptional and adipocyte physiology profiling in vitro and in vivo, we could not detect an effect of gain or loss of function of <i>Gm15551</i>. |
| first_indexed | 2024-03-09T22:52:09Z |
| format | Article |
| id | doaj.art-3ed3d1a9757b48f5bec78e3a79ad1874 |
| institution | Directory Open Access Journal |
| issn | 2311-553X |
| language | English |
| last_indexed | 2024-03-09T22:52:09Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Non-Coding RNA |
| spelling | doaj.art-3ed3d1a9757b48f5bec78e3a79ad18742023-11-23T18:18:06ZengMDPI AGNon-Coding RNA2311-553X2022-05-01833210.3390/ncrna8030032Comprehensive Transcriptional Profiling and Mouse Phenotyping Reveals Dispensable Role for Adipose Tissue Selective Long Noncoding RNA <i>Gm15551</i>Christoph Andreas Engelhard0Chien Huang1Sajjad Khani2Petr Kasparek3Jan Prochazka4Jan Rozman5David Pajuelo Reguera6Radislav Sedlacek7Jan-Wilhelm Kornfeld8Department for Biochemistry and Molecular Biology (BMB), University of Southern Denmark, Campusvej 55, 5230 Odense, DenmarkDepartment for Biochemistry and Molecular Biology (BMB), University of Southern Denmark, Campusvej 55, 5230 Odense, DenmarkMax Planck Institute for Metabolism Research, Gleueler Strasse 50, 50931 Köln, GermanyCzech Centre for Phenogenomics, Institute of Molecular Genetics of the Czech Academy of Sciences, Prumyslova 595, 25250 Vestec, Czech RepublicCzech Centre for Phenogenomics, Institute of Molecular Genetics of the Czech Academy of Sciences, Prumyslova 595, 25250 Vestec, Czech RepublicCzech Centre for Phenogenomics, Institute of Molecular Genetics of the Czech Academy of Sciences, Prumyslova 595, 25250 Vestec, Czech RepublicCzech Centre for Phenogenomics, Institute of Molecular Genetics of the Czech Academy of Sciences, Prumyslova 595, 25250 Vestec, Czech RepublicCzech Centre for Phenogenomics, Institute of Molecular Genetics of the Czech Academy of Sciences, Prumyslova 595, 25250 Vestec, Czech RepublicDepartment for Biochemistry and Molecular Biology (BMB), University of Southern Denmark, Campusvej 55, 5230 Odense, DenmarkCold and nutrient-activated brown adipose tissue (BAT) is capable of increasing systemic energy expenditure via the uncoupled respiration and secretion of endocrine factors, thereby protecting mice against diet-induced obesity and improving insulin response and glucose tolerance in men. Long non-coding RNAs (lncRNAs) have recently been identified as fine-tuning regulators of cellular function. While certain lncRNAs have been functionally characterised in adipose tissue, their overall contribution in the activation of BAT remains elusive. We identified lncRNAs correlating to interscapular brown adipose tissue (iBAT) function in a high fat diet (HFD) and cold stressed mice. We focused on <i>Gm15551</i>, which has an adipose tissue specific expression profile, is highly upregulated during adipogenesis, and downregulated by β-adrenergic activation in mature adipocytes. Although we performed comprehensive transcriptional and adipocyte physiology profiling in vitro and in vivo, we could not detect an effect of gain or loss of function of <i>Gm15551</i>.https://www.mdpi.com/2311-553X/8/3/32long noncoding RNAsbrown adipose tissueadipose tissue remodelling |
| spellingShingle | Christoph Andreas Engelhard Chien Huang Sajjad Khani Petr Kasparek Jan Prochazka Jan Rozman David Pajuelo Reguera Radislav Sedlacek Jan-Wilhelm Kornfeld Comprehensive Transcriptional Profiling and Mouse Phenotyping Reveals Dispensable Role for Adipose Tissue Selective Long Noncoding RNA <i>Gm15551</i> Non-Coding RNA long noncoding RNAs brown adipose tissue adipose tissue remodelling |
| title | Comprehensive Transcriptional Profiling and Mouse Phenotyping Reveals Dispensable Role for Adipose Tissue Selective Long Noncoding RNA <i>Gm15551</i> |
| title_full | Comprehensive Transcriptional Profiling and Mouse Phenotyping Reveals Dispensable Role for Adipose Tissue Selective Long Noncoding RNA <i>Gm15551</i> |
| title_fullStr | Comprehensive Transcriptional Profiling and Mouse Phenotyping Reveals Dispensable Role for Adipose Tissue Selective Long Noncoding RNA <i>Gm15551</i> |
| title_full_unstemmed | Comprehensive Transcriptional Profiling and Mouse Phenotyping Reveals Dispensable Role for Adipose Tissue Selective Long Noncoding RNA <i>Gm15551</i> |
| title_short | Comprehensive Transcriptional Profiling and Mouse Phenotyping Reveals Dispensable Role for Adipose Tissue Selective Long Noncoding RNA <i>Gm15551</i> |
| title_sort | comprehensive transcriptional profiling and mouse phenotyping reveals dispensable role for adipose tissue selective long noncoding rna i gm15551 i |
| topic | long noncoding RNAs brown adipose tissue adipose tissue remodelling |
| url | https://www.mdpi.com/2311-553X/8/3/32 |
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