Q fever: A neglected disease of camels in Giza and Cairo Provinces, Egypt
Background and Aim: Q fever is a zoonotic disease caused by Coxiella burnetii. Cattle, sheep, and goat are the main reservoir of C. burnetii. In Egypt, the epidemiological data about C. burnetii in camels are limited. Therefore, the current study was conducted to identify C. burnetii infection in ca...
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| Format: | Article |
| Language: | English |
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Veterinary World
2019-12-01
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| Series: | Veterinary World |
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| Online Access: | http://www.veterinaryworld.org/Vol.12/December-2019/9.pdf |
| _version_ | 1818455756429590528 |
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| author | Hend H. A. M. Abdullah Hany A. Hussein Khaled A. Abd El-Razik Ashraf M. A. Barakat Yousef A. Soliman |
| author_facet | Hend H. A. M. Abdullah Hany A. Hussein Khaled A. Abd El-Razik Ashraf M. A. Barakat Yousef A. Soliman |
| author_sort | Hend H. A. M. Abdullah |
| collection | DOAJ |
| description | Background and Aim: Q fever is a zoonotic disease caused by Coxiella burnetii. Cattle, sheep, and goat are the main reservoir of C. burnetii. In Egypt, the epidemiological data about C. burnetii in camels are limited. Therefore, the current study was conducted to identify C. burnetii infection in camels by different molecular tools and to estimate its seropositivity through the detection of anti-C. burnetii antibodies in camel sera.
Materials and Methods: Blood samples were collected 112 from camels in Giza and Cairo Provinces, Egypt. All blood samples were screened by trans-quantitative polymerase chain reaction (trans-qPCR) for C. burnetii and positive samples subjected to standard PCR using the superoxide dismutase enzyme coding gene of C. burnetii. Sera of studied camels were examined for the presence of antibodies against C. burnetii using enzyme-linked immunosorbent assay.
Results: Out of 112 camels, 19 were positive for C. burnetii by qPCR with an overall prevalence of 16.9% (18.6% in Giza and 15.1% in Cairo Provinces, respectively). The seroprevalence of anti-C. burnetii IgG antibodies in the examined camels was 4.5% (5/112).
Conclusion: Trans-qPCR assay is a rapid and sensitive tool for the detection of C. burnetii in acute stage. Camels should be considered one of the major reservoirs for C. burnetii in Egypt. |
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| id | doaj.art-3ed4e9c2a0e3498f93d624f8d09c403c |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-12-14T22:15:50Z |
| publishDate | 2019-12-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-3ed4e9c2a0e3498f93d624f8d09c403c2022-12-21T22:45:38ZengVeterinary WorldVeterinary World0972-89882231-09162019-12-0112121945195010.14202/vetworld.2019.1945-1950Q fever: A neglected disease of camels in Giza and Cairo Provinces, EgyptHend H. A. M. Abdullah0Hany A. Hussein1Khaled A. Abd El-Razik2Ashraf M. A. Barakat3Yousef A. Soliman4Department of Parasitology and Animal Diseases, National Research Centre, Dokki, Giza, Egypt.Department of Animal Reproduction and Artificial Insemination, National Research Centre, Dokki, Giza, Egypt; Key Laboratory of Diagnostic and Detective Technology, Department of Veterinary Research , Guangdong Haid Institute of Animal Husbandry and Veterinary, Guangzhou, China.Department of Animal Reproduction and Artificial Insemination, National Research Centre, Dokki, Giza, Egypt.Department of Zoonotic Diseases, National Research Centre, Dokki, Giza, Egypt.Department of Biotechnology, Central Laboratory for Evaluation of Veterinary Biologics, Abbasia, Cairo, Egypt.Background and Aim: Q fever is a zoonotic disease caused by Coxiella burnetii. Cattle, sheep, and goat are the main reservoir of C. burnetii. In Egypt, the epidemiological data about C. burnetii in camels are limited. Therefore, the current study was conducted to identify C. burnetii infection in camels by different molecular tools and to estimate its seropositivity through the detection of anti-C. burnetii antibodies in camel sera. Materials and Methods: Blood samples were collected 112 from camels in Giza and Cairo Provinces, Egypt. All blood samples were screened by trans-quantitative polymerase chain reaction (trans-qPCR) for C. burnetii and positive samples subjected to standard PCR using the superoxide dismutase enzyme coding gene of C. burnetii. Sera of studied camels were examined for the presence of antibodies against C. burnetii using enzyme-linked immunosorbent assay. Results: Out of 112 camels, 19 were positive for C. burnetii by qPCR with an overall prevalence of 16.9% (18.6% in Giza and 15.1% in Cairo Provinces, respectively). The seroprevalence of anti-C. burnetii IgG antibodies in the examined camels was 4.5% (5/112). Conclusion: Trans-qPCR assay is a rapid and sensitive tool for the detection of C. burnetii in acute stage. Camels should be considered one of the major reservoirs for C. burnetii in Egypt.http://www.veterinaryworld.org/Vol.12/December-2019/9.pdfcamelcoxiella burnetiienzyme-linked immunosorbent assaystandard polymerase chain reactiontrans-quantitative polymerase chain reaction |
| spellingShingle | Hend H. A. M. Abdullah Hany A. Hussein Khaled A. Abd El-Razik Ashraf M. A. Barakat Yousef A. Soliman Q fever: A neglected disease of camels in Giza and Cairo Provinces, Egypt Veterinary World camel coxiella burnetii enzyme-linked immunosorbent assay standard polymerase chain reaction trans-quantitative polymerase chain reaction |
| title | Q fever: A neglected disease of camels in Giza and Cairo Provinces, Egypt |
| title_full | Q fever: A neglected disease of camels in Giza and Cairo Provinces, Egypt |
| title_fullStr | Q fever: A neglected disease of camels in Giza and Cairo Provinces, Egypt |
| title_full_unstemmed | Q fever: A neglected disease of camels in Giza and Cairo Provinces, Egypt |
| title_short | Q fever: A neglected disease of camels in Giza and Cairo Provinces, Egypt |
| title_sort | q fever a neglected disease of camels in giza and cairo provinces egypt |
| topic | camel coxiella burnetii enzyme-linked immunosorbent assay standard polymerase chain reaction trans-quantitative polymerase chain reaction |
| url | http://www.veterinaryworld.org/Vol.12/December-2019/9.pdf |
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