Using a Relative Quantitative Proteomic Method to Identify Differentially Abundant Proteins in Brucella melitensis Biovar 3 and Brucella melitensis M5-90
Brucellosis, caused by Brucella spp., is one of the most widespread bacterial zoonoses worldwide. Vaccination is still considered the best way to control brucellosis. An investigation into the differential proteome expression patterns of wild and vaccine strains may help researchers and clinicians d...
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-07-01
|
| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2022.929040/full |
| _version_ | 1818196508875423744 |
|---|---|
| author | Huan Zhang Yueli Wang Yifan Wang Xiaoyu Deng Taiwang Ji Zhongchen Ma Ningning Yang Mingguo Xu Honghuan Li Jihai Yi Yong Wang Yuanzhi Wang Jinliang Sheng Zhen Wang Chuangfu Chen |
| author_facet | Huan Zhang Yueli Wang Yifan Wang Xiaoyu Deng Taiwang Ji Zhongchen Ma Ningning Yang Mingguo Xu Honghuan Li Jihai Yi Yong Wang Yuanzhi Wang Jinliang Sheng Zhen Wang Chuangfu Chen |
| author_sort | Huan Zhang |
| collection | DOAJ |
| description | Brucellosis, caused by Brucella spp., is one of the most widespread bacterial zoonoses worldwide. Vaccination is still considered the best way to control brucellosis. An investigation into the differential proteome expression patterns of wild and vaccine strains may help researchers and clinicians differentiate between the strains to diagnose and better understand the mechanism(s) underlying differences in virulence. In the present study, a mass spectrometry-based, label-free relative quantitative proteomics approach was used to investigate the proteins expressed by the wild strain, B. melitensis biovar 3 and compare it with those expressed by B. melitensis M5-90. The higher level of virulence for B. melitensis biovar 3 compared to B. melitensis M5-90 was validated in vitro and in vivo. A total of 2133 proteins, encompassing 68% of the theoretical proteome, were identified and quantified by proteomic analysis, resulting in broad coverage of the B. melitensis proteome. A total of 147 proteins were identified as differentially expressed (DE) between these two strains. In addition, 9 proteins and 30 proteins were identified as unique to B. melitensis M5-90 and B. melitensis biovar 3, respectively. Pathway analysis revealed that the majority of the DE proteins were involved in iron uptake, quorum sensing, pyrimidine metabolism, glycine betaine biosynthetic and metabolic processes, thiamine-containing compound metabolism and ABC transporters. The expression of BtpA and VjbR proteins (two well-known virulence factors) in B. melitensis biovar 3 was 8-fold and 2-fold higher than in B. melitensis M5-90. In summary, our results identified many unique proteins that could be selected as candidate markers for differentiating vaccinated animals from animals with wild-type infections. BtpA and VjbR proteins might be responsible for the residual virulence of B. melitensis M5-90, while ABC transporters and thiamine metabolism associated proteins may be newly identified Brucella virulence factors. All of the identified DE proteins provide valuable information for the development of vaccines and the discovery of novel therapeutic targets. |
| first_indexed | 2024-12-12T01:35:12Z |
| format | Article |
| id | doaj.art-3ed5d12a4dad4e5a98e1da5cebfc46b7 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-12T01:35:12Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-3ed5d12a4dad4e5a98e1da5cebfc46b72022-12-22T00:42:52ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-07-011310.3389/fimmu.2022.929040929040Using a Relative Quantitative Proteomic Method to Identify Differentially Abundant Proteins in Brucella melitensis Biovar 3 and Brucella melitensis M5-90Huan Zhang0Yueli Wang1Yifan Wang2Xiaoyu Deng3Taiwang Ji4Zhongchen Ma5Ningning Yang6Mingguo Xu7Honghuan Li8Jihai Yi9Yong Wang10Yuanzhi Wang11Jinliang Sheng12Zhen Wang13Chuangfu Chen14School of Animal Science and Technology, Shihezi University, Shihezi City, ChinaSchool of Animal Science and Technology, Shihezi University, Shihezi City, ChinaState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine Huazhong Agricultural University, Wuhan, ChinaSchool of Animal Science and Technology, Shihezi University, Shihezi City, ChinaSchool of Animal Science and Technology, Shihezi University, Shihezi City, ChinaSchool of Animal Science and Technology, Shihezi University, Shihezi City, ChinaSchool of Animal Science and Technology, Shihezi University, Shihezi City, ChinaSchool of Animal Science and Technology, Shihezi University, Shihezi City, ChinaSchool of Animal Science and Technology, Shihezi University, Shihezi City, ChinaSchool of Animal Science and Technology, Shihezi University, Shihezi City, ChinaSchool of Animal Science and Technology, Shihezi University, Shihezi City, ChinaSchool of Medicine, Shihezi University, Shihezi City, ChinaSchool of Animal Science and Technology, Shihezi University, Shihezi City, ChinaSchool of Animal Science and Technology, Shihezi University, Shihezi City, ChinaSchool of Animal Science and Technology, Shihezi University, Shihezi City, ChinaBrucellosis, caused by Brucella spp., is one of the most widespread bacterial zoonoses worldwide. Vaccination is still considered the best way to control brucellosis. An investigation into the differential proteome expression patterns of wild and vaccine strains may help researchers and clinicians differentiate between the strains to diagnose and better understand the mechanism(s) underlying differences in virulence. In the present study, a mass spectrometry-based, label-free relative quantitative proteomics approach was used to investigate the proteins expressed by the wild strain, B. melitensis biovar 3 and compare it with those expressed by B. melitensis M5-90. The higher level of virulence for B. melitensis biovar 3 compared to B. melitensis M5-90 was validated in vitro and in vivo. A total of 2133 proteins, encompassing 68% of the theoretical proteome, were identified and quantified by proteomic analysis, resulting in broad coverage of the B. melitensis proteome. A total of 147 proteins were identified as differentially expressed (DE) between these two strains. In addition, 9 proteins and 30 proteins were identified as unique to B. melitensis M5-90 and B. melitensis biovar 3, respectively. Pathway analysis revealed that the majority of the DE proteins were involved in iron uptake, quorum sensing, pyrimidine metabolism, glycine betaine biosynthetic and metabolic processes, thiamine-containing compound metabolism and ABC transporters. The expression of BtpA and VjbR proteins (two well-known virulence factors) in B. melitensis biovar 3 was 8-fold and 2-fold higher than in B. melitensis M5-90. In summary, our results identified many unique proteins that could be selected as candidate markers for differentiating vaccinated animals from animals with wild-type infections. BtpA and VjbR proteins might be responsible for the residual virulence of B. melitensis M5-90, while ABC transporters and thiamine metabolism associated proteins may be newly identified Brucella virulence factors. All of the identified DE proteins provide valuable information for the development of vaccines and the discovery of novel therapeutic targets.https://www.frontiersin.org/articles/10.3389/fimmu.2022.929040/fullBrucellosisBrucella melitensis M5-90Brucella melitensis biovar 3proteomicsvaccine |
| spellingShingle | Huan Zhang Yueli Wang Yifan Wang Xiaoyu Deng Taiwang Ji Zhongchen Ma Ningning Yang Mingguo Xu Honghuan Li Jihai Yi Yong Wang Yuanzhi Wang Jinliang Sheng Zhen Wang Chuangfu Chen Using a Relative Quantitative Proteomic Method to Identify Differentially Abundant Proteins in Brucella melitensis Biovar 3 and Brucella melitensis M5-90 Frontiers in Immunology Brucellosis Brucella melitensis M5-90 Brucella melitensis biovar 3 proteomics vaccine |
| title | Using a Relative Quantitative Proteomic Method to Identify Differentially Abundant Proteins in Brucella melitensis Biovar 3 and Brucella melitensis M5-90 |
| title_full | Using a Relative Quantitative Proteomic Method to Identify Differentially Abundant Proteins in Brucella melitensis Biovar 3 and Brucella melitensis M5-90 |
| title_fullStr | Using a Relative Quantitative Proteomic Method to Identify Differentially Abundant Proteins in Brucella melitensis Biovar 3 and Brucella melitensis M5-90 |
| title_full_unstemmed | Using a Relative Quantitative Proteomic Method to Identify Differentially Abundant Proteins in Brucella melitensis Biovar 3 and Brucella melitensis M5-90 |
| title_short | Using a Relative Quantitative Proteomic Method to Identify Differentially Abundant Proteins in Brucella melitensis Biovar 3 and Brucella melitensis M5-90 |
| title_sort | using a relative quantitative proteomic method to identify differentially abundant proteins in brucella melitensis biovar 3 and brucella melitensis m5 90 |
| topic | Brucellosis Brucella melitensis M5-90 Brucella melitensis biovar 3 proteomics vaccine |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2022.929040/full |
| work_keys_str_mv | AT huanzhang usingarelativequantitativeproteomicmethodtoidentifydifferentiallyabundantproteinsinbrucellamelitensisbiovar3andbrucellamelitensism590 AT yueliwang usingarelativequantitativeproteomicmethodtoidentifydifferentiallyabundantproteinsinbrucellamelitensisbiovar3andbrucellamelitensism590 AT yifanwang usingarelativequantitativeproteomicmethodtoidentifydifferentiallyabundantproteinsinbrucellamelitensisbiovar3andbrucellamelitensism590 AT xiaoyudeng usingarelativequantitativeproteomicmethodtoidentifydifferentiallyabundantproteinsinbrucellamelitensisbiovar3andbrucellamelitensism590 AT taiwangji usingarelativequantitativeproteomicmethodtoidentifydifferentiallyabundantproteinsinbrucellamelitensisbiovar3andbrucellamelitensism590 AT zhongchenma usingarelativequantitativeproteomicmethodtoidentifydifferentiallyabundantproteinsinbrucellamelitensisbiovar3andbrucellamelitensism590 AT ningningyang usingarelativequantitativeproteomicmethodtoidentifydifferentiallyabundantproteinsinbrucellamelitensisbiovar3andbrucellamelitensism590 AT mingguoxu usingarelativequantitativeproteomicmethodtoidentifydifferentiallyabundantproteinsinbrucellamelitensisbiovar3andbrucellamelitensism590 AT honghuanli usingarelativequantitativeproteomicmethodtoidentifydifferentiallyabundantproteinsinbrucellamelitensisbiovar3andbrucellamelitensism590 AT jihaiyi usingarelativequantitativeproteomicmethodtoidentifydifferentiallyabundantproteinsinbrucellamelitensisbiovar3andbrucellamelitensism590 AT yongwang usingarelativequantitativeproteomicmethodtoidentifydifferentiallyabundantproteinsinbrucellamelitensisbiovar3andbrucellamelitensism590 AT yuanzhiwang usingarelativequantitativeproteomicmethodtoidentifydifferentiallyabundantproteinsinbrucellamelitensisbiovar3andbrucellamelitensism590 AT jinliangsheng usingarelativequantitativeproteomicmethodtoidentifydifferentiallyabundantproteinsinbrucellamelitensisbiovar3andbrucellamelitensism590 AT zhenwang usingarelativequantitativeproteomicmethodtoidentifydifferentiallyabundantproteinsinbrucellamelitensisbiovar3andbrucellamelitensism590 AT chuangfuchen usingarelativequantitativeproteomicmethodtoidentifydifferentiallyabundantproteinsinbrucellamelitensisbiovar3andbrucellamelitensism590 |