Protocol for recording and analyzing spontaneous locomotion in Drosophila
Summary: The quantitative analysis of locomotion is used to study many biological processes. Here, we describe how to record the locomotion of up to 50 Drosophila individuals and process the resulting video files using FlyTracker. We detail the use of modifiable MatLab scripts to process structure a...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-12-01
|
| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722007687 |
| _version_ | 1798005200760340480 |
|---|---|
| author | Taylor Barwell Sehaj Raina Laurent Seroude |
| author_facet | Taylor Barwell Sehaj Raina Laurent Seroude |
| author_sort | Taylor Barwell |
| collection | DOAJ |
| description | Summary: The quantitative analysis of locomotion is used to study many biological processes. Here, we describe how to record the locomotion of up to 50 Drosophila individuals and process the resulting video files using FlyTracker. We detail the use of modifiable MatLab scripts to process structure array files generated by FlyTracker. We have applied this to study Drosophila movement during aging, but it could be used to address a variety of research questions.For complete details on the use and execution of this protocol, please refer to Barwell et al. (2021).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-11T12:36:32Z |
| format | Article |
| id | doaj.art-3ed7115bff9742988bd88bd645ccda8a |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-11T12:36:32Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-3ed7115bff9742988bd88bd645ccda8a2022-12-22T04:23:37ZengElsevierSTAR Protocols2666-16672022-12-0134101888Protocol for recording and analyzing spontaneous locomotion in DrosophilaTaylor Barwell0Sehaj Raina1Laurent Seroude2Department of Biology, Queen’s University, BioSciences Complex, Kingston, ON K7L 3N6, Canada; Corresponding authorDepartment of Biology, Queen’s University, BioSciences Complex, Kingston, ON K7L 3N6, Canada; Department of Biology, York University, Life Sciences Building, Toronto, ON M3J 1P3, CanadaDepartment of Biology, Queen’s University, BioSciences Complex, Kingston, ON K7L 3N6, Canada; Corresponding authorSummary: The quantitative analysis of locomotion is used to study many biological processes. Here, we describe how to record the locomotion of up to 50 Drosophila individuals and process the resulting video files using FlyTracker. We detail the use of modifiable MatLab scripts to process structure array files generated by FlyTracker. We have applied this to study Drosophila movement during aging, but it could be used to address a variety of research questions.For complete details on the use and execution of this protocol, please refer to Barwell et al. (2021).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166722007687GeneticsModel OrganismsNeuroscienceBehavior |
| spellingShingle | Taylor Barwell Sehaj Raina Laurent Seroude Protocol for recording and analyzing spontaneous locomotion in Drosophila STAR Protocols Genetics Model Organisms Neuroscience Behavior |
| title | Protocol for recording and analyzing spontaneous locomotion in Drosophila |
| title_full | Protocol for recording and analyzing spontaneous locomotion in Drosophila |
| title_fullStr | Protocol for recording and analyzing spontaneous locomotion in Drosophila |
| title_full_unstemmed | Protocol for recording and analyzing spontaneous locomotion in Drosophila |
| title_short | Protocol for recording and analyzing spontaneous locomotion in Drosophila |
| title_sort | protocol for recording and analyzing spontaneous locomotion in drosophila |
| topic | Genetics Model Organisms Neuroscience Behavior |
| url | http://www.sciencedirect.com/science/article/pii/S2666166722007687 |
| work_keys_str_mv | AT taylorbarwell protocolforrecordingandanalyzingspontaneouslocomotionindrosophila AT sehajraina protocolforrecordingandanalyzingspontaneouslocomotionindrosophila AT laurentseroude protocolforrecordingandanalyzingspontaneouslocomotionindrosophila |