Preventive effect of Terminalia bellirica (Gaertn.) Roxb. extract on mice infected with Salmonella Typhimurium
Terminalia bellirica (Gaertn.) Roxb. (TB) is a traditional herbal combination used in Chinese medicine for the treatment of a broad range of diseases. In this study, thirty KM mice were randomly divided into control (N), infection group (NS), and the TB protection group (HS). Based on its digestive...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2022.1054205/full |
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| author | Qinghui Kong Qinghui Kong Zhenda Shang Zhenda Shang Yao Liu Muhammad Fakhar-e-Alam Kulyar Sizhu Suo-lang Yefen Xu Zhankun Tan Zhankun Tan Jiakui Li Jiakui Li Suozhu Liu Suozhu Liu |
| author_facet | Qinghui Kong Qinghui Kong Zhenda Shang Zhenda Shang Yao Liu Muhammad Fakhar-e-Alam Kulyar Sizhu Suo-lang Yefen Xu Zhankun Tan Zhankun Tan Jiakui Li Jiakui Li Suozhu Liu Suozhu Liu |
| author_sort | Qinghui Kong |
| collection | DOAJ |
| description | Terminalia bellirica (Gaertn.) Roxb. (TB) is a traditional herbal combination used in Chinese medicine for the treatment of a broad range of diseases. In this study, thirty KM mice were randomly divided into control (N), infection group (NS), and the TB protection group (HS). Based on its digestive feature, intestinal physical barrier, immunological barrier and gut microbiota effects in vivo on challenged with S.typhimurium mice were investigated after oral administration of 600 mg/kg b.wt of TB for 13 days. The results show that the extract could improve the level of serum immunoglobulins (IgA and IgG), decrease the intestinal cytokine secretion to relieve intestinal cytokine storm, reinforce the intestinal biochemical barrier function by elevating the sIgA expression, and strengthen the intestinal physical barrier function. Simultaneously, based on the V3–V4 region of the 16S rRNA analyzed, the results of the taxonomic structure of the intestinal microbiota demonstrated that the TB prevention effect transformed the key phylotypes of the gut microbiota in S. Typhimurium-challenged mice and promoted the multiplication of beneficial bacteria. Furthermore, the abundance of Firmicutes and Deferribacteres increased, while that of Bacteroidetes and Actinobacteria decreased. At the genus level, the abundance of Ruminococcus and Oscillospira was substantially enhanced, while the other dominant genera showed no significant change between the vehicle control groups and the TB prevention groups. In summary, these results provide evidence that the administration of TB extract can prevent S. Typhimurium infection by alleviating the intestinal physical and immunological barriers and normalizing the gut microbiota, highlighting a promising application in clinical treatment. Thus, our results provide new insights into the biological functions of TB for the preventive effect of intestinal inflammation. |
| first_indexed | 2024-04-11T00:07:31Z |
| format | Article |
| id | doaj.art-3eda399f904d4d128bd41d9af662f969 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-04-11T00:07:31Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-3eda399f904d4d128bd41d9af662f9692023-01-09T10:40:37ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882023-01-011210.3389/fcimb.2022.10542051054205Preventive effect of Terminalia bellirica (Gaertn.) Roxb. extract on mice infected with Salmonella TyphimuriumQinghui Kong0Qinghui Kong1Zhenda Shang2Zhenda Shang3Yao Liu4Muhammad Fakhar-e-Alam Kulyar5Sizhu Suo-lang6Yefen Xu7Zhankun Tan8Zhankun Tan9Jiakui Li10Jiakui Li11Suozhu Liu12Suozhu Liu13College of Animal Science, Tibet Agricultural and Animal Husbandry University, Nyingchi, ChinaCollege of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaCollege of Animal Science, Tibet Agricultural and Animal Husbandry University, Nyingchi, ChinaTibetan Plateau Feed Processing Engineering Research Center, Nyingchi, ChinaCollege of Animal Science, Tibet Agricultural and Animal Husbandry University, Nyingchi, ChinaCollege of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaCollege of Animal Science, Tibet Agricultural and Animal Husbandry University, Nyingchi, ChinaCollege of Animal Science, Tibet Agricultural and Animal Husbandry University, Nyingchi, ChinaCollege of Animal Science, Tibet Agricultural and Animal Husbandry University, Nyingchi, ChinaTibetan Plateau Feed Processing Engineering Research Center, Nyingchi, ChinaCollege of Animal Science, Tibet Agricultural and Animal Husbandry University, Nyingchi, ChinaCollege of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaCollege of Animal Science, Tibet Agricultural and Animal Husbandry University, Nyingchi, ChinaTibetan Plateau Feed Processing Engineering Research Center, Nyingchi, ChinaTerminalia bellirica (Gaertn.) Roxb. (TB) is a traditional herbal combination used in Chinese medicine for the treatment of a broad range of diseases. In this study, thirty KM mice were randomly divided into control (N), infection group (NS), and the TB protection group (HS). Based on its digestive feature, intestinal physical barrier, immunological barrier and gut microbiota effects in vivo on challenged with S.typhimurium mice were investigated after oral administration of 600 mg/kg b.wt of TB for 13 days. The results show that the extract could improve the level of serum immunoglobulins (IgA and IgG), decrease the intestinal cytokine secretion to relieve intestinal cytokine storm, reinforce the intestinal biochemical barrier function by elevating the sIgA expression, and strengthen the intestinal physical barrier function. Simultaneously, based on the V3–V4 region of the 16S rRNA analyzed, the results of the taxonomic structure of the intestinal microbiota demonstrated that the TB prevention effect transformed the key phylotypes of the gut microbiota in S. Typhimurium-challenged mice and promoted the multiplication of beneficial bacteria. Furthermore, the abundance of Firmicutes and Deferribacteres increased, while that of Bacteroidetes and Actinobacteria decreased. At the genus level, the abundance of Ruminococcus and Oscillospira was substantially enhanced, while the other dominant genera showed no significant change between the vehicle control groups and the TB prevention groups. In summary, these results provide evidence that the administration of TB extract can prevent S. Typhimurium infection by alleviating the intestinal physical and immunological barriers and normalizing the gut microbiota, highlighting a promising application in clinical treatment. Thus, our results provide new insights into the biological functions of TB for the preventive effect of intestinal inflammation.https://www.frontiersin.org/articles/10.3389/fcimb.2022.1054205/fullTerminalia bellirica (Gaertn.) Roxb. extractmiceSalmonella Typhimuriumpreventive effecttraditional herbal medicine |
| spellingShingle | Qinghui Kong Qinghui Kong Zhenda Shang Zhenda Shang Yao Liu Muhammad Fakhar-e-Alam Kulyar Sizhu Suo-lang Yefen Xu Zhankun Tan Zhankun Tan Jiakui Li Jiakui Li Suozhu Liu Suozhu Liu Preventive effect of Terminalia bellirica (Gaertn.) Roxb. extract on mice infected with Salmonella Typhimurium Frontiers in Cellular and Infection Microbiology Terminalia bellirica (Gaertn.) Roxb. extract mice Salmonella Typhimurium preventive effect traditional herbal medicine |
| title | Preventive effect of Terminalia bellirica (Gaertn.) Roxb. extract on mice infected with Salmonella Typhimurium |
| title_full | Preventive effect of Terminalia bellirica (Gaertn.) Roxb. extract on mice infected with Salmonella Typhimurium |
| title_fullStr | Preventive effect of Terminalia bellirica (Gaertn.) Roxb. extract on mice infected with Salmonella Typhimurium |
| title_full_unstemmed | Preventive effect of Terminalia bellirica (Gaertn.) Roxb. extract on mice infected with Salmonella Typhimurium |
| title_short | Preventive effect of Terminalia bellirica (Gaertn.) Roxb. extract on mice infected with Salmonella Typhimurium |
| title_sort | preventive effect of terminalia bellirica gaertn roxb extract on mice infected with salmonella typhimurium |
| topic | Terminalia bellirica (Gaertn.) Roxb. extract mice Salmonella Typhimurium preventive effect traditional herbal medicine |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2022.1054205/full |
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