Development and validation of a high performance liquid chromatographic method for determination of cyclosporine-A from biodegradable intraocular implants
An HPLC method was developed and validated aiming to quantify the cyclosporine-A incorporated into intraocular implants, released from them; and in direct contact with the degradation products of PLGA. The separation was carried out in isocratic mode using acetonitrile/water (70:30) as mobile phase,...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Química
2011-01-01
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| Series: | Química Nova |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422011000100026&lng=en&tlng=en |
| _version_ | 1818196860856172544 |
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| author | Juliana Barbosa Saliba Armando da Silva Cunha Junior Elionai Cassiana de Lima Gomes Herman Sander Mansur Gisele Rodrigues da Silva |
| author_facet | Juliana Barbosa Saliba Armando da Silva Cunha Junior Elionai Cassiana de Lima Gomes Herman Sander Mansur Gisele Rodrigues da Silva |
| author_sort | Juliana Barbosa Saliba |
| collection | DOAJ |
| description | An HPLC method was developed and validated aiming to quantify the cyclosporine-A incorporated into intraocular implants, released from them; and in direct contact with the degradation products of PLGA. The separation was carried out in isocratic mode using acetonitrile/water (70:30) as mobile phase, a C18 column at 80 ºC and UV detection at 210 nm. The method provided selectivity based on resolution among peaks. It was linear over the range of 2.5-40.0 µg/mL. The quantitation and detection limits were 0.8 and 1.2 µg/mL, respectively. The recovery was 101.8% and intra-day and inter-day precision was close to 2%. |
| first_indexed | 2024-12-12T01:40:48Z |
| format | Article |
| id | doaj.art-3edb261425be41f1b2c921a53607f72e |
| institution | Directory Open Access Journal |
| issn | 1678-7064 |
| language | English |
| last_indexed | 2024-12-12T01:40:48Z |
| publishDate | 2011-01-01 |
| publisher | Sociedade Brasileira de Química |
| record_format | Article |
| series | Química Nova |
| spelling | doaj.art-3edb261425be41f1b2c921a53607f72e2022-12-22T00:42:43ZengSociedade Brasileira de QuímicaQuímica Nova1678-70642011-01-0134114014410.1590/S0100-40422011000100026S0100-40422011000100026Development and validation of a high performance liquid chromatographic method for determination of cyclosporine-A from biodegradable intraocular implantsJuliana Barbosa Saliba0Armando da Silva Cunha Junior1Elionai Cassiana de Lima Gomes2Herman Sander Mansur3Gisele Rodrigues da Silva4Universidade Federal de Minas GeraisUniversidade Federal de Minas GeraisUniversidade Federal de Minas GeraisUniversidade Federal de Minas GeraisUniversidade Federal de São João del-ReiAn HPLC method was developed and validated aiming to quantify the cyclosporine-A incorporated into intraocular implants, released from them; and in direct contact with the degradation products of PLGA. The separation was carried out in isocratic mode using acetonitrile/water (70:30) as mobile phase, a C18 column at 80 ºC and UV detection at 210 nm. The method provided selectivity based on resolution among peaks. It was linear over the range of 2.5-40.0 µg/mL. The quantitation and detection limits were 0.8 and 1.2 µg/mL, respectively. The recovery was 101.8% and intra-day and inter-day precision was close to 2%.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422011000100026&lng=en&tlng=encyclosporine-Abiodegradable intraocular implantvalidation |
| spellingShingle | Juliana Barbosa Saliba Armando da Silva Cunha Junior Elionai Cassiana de Lima Gomes Herman Sander Mansur Gisele Rodrigues da Silva Development and validation of a high performance liquid chromatographic method for determination of cyclosporine-A from biodegradable intraocular implants Química Nova cyclosporine-A biodegradable intraocular implant validation |
| title | Development and validation of a high performance liquid chromatographic method for determination of cyclosporine-A from biodegradable intraocular implants |
| title_full | Development and validation of a high performance liquid chromatographic method for determination of cyclosporine-A from biodegradable intraocular implants |
| title_fullStr | Development and validation of a high performance liquid chromatographic method for determination of cyclosporine-A from biodegradable intraocular implants |
| title_full_unstemmed | Development and validation of a high performance liquid chromatographic method for determination of cyclosporine-A from biodegradable intraocular implants |
| title_short | Development and validation of a high performance liquid chromatographic method for determination of cyclosporine-A from biodegradable intraocular implants |
| title_sort | development and validation of a high performance liquid chromatographic method for determination of cyclosporine a from biodegradable intraocular implants |
| topic | cyclosporine-A biodegradable intraocular implant validation |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422011000100026&lng=en&tlng=en |
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