Synchronous and asynchronous modes of synaptic transmission utilize different calcium sources
Asynchronous transmission plays a prominent role at certain synapses but lacks the mechanistic insights of its synchronous counterpart. The current view posits that triggering of asynchronous release during repetitive stimulation involves expansion of the same calcium domains underlying synchronous...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2013-12-01
|
| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/01206 |
| _version_ | 1811200094800707584 |
|---|---|
| author | Hua Wen Jeffrey M Hubbard Benjamin Rakela Michael W Linhoff Gail Mandel Paul Brehm |
| author_facet | Hua Wen Jeffrey M Hubbard Benjamin Rakela Michael W Linhoff Gail Mandel Paul Brehm |
| author_sort | Hua Wen |
| collection | DOAJ |
| description | Asynchronous transmission plays a prominent role at certain synapses but lacks the mechanistic insights of its synchronous counterpart. The current view posits that triggering of asynchronous release during repetitive stimulation involves expansion of the same calcium domains underlying synchronous transmission. In this study, live imaging and paired patch clamp recording at the zebrafish neuromuscular synapse reveal contributions by spatially distinct calcium sources. Synchronous release is tied to calcium entry into synaptic boutons via P/Q type calcium channels, whereas asynchronous release is boosted by a propagating intracellular calcium source initiated at off-synaptic locations in the axon and axonal branch points. This secondary calcium source fully accounts for the persistence following termination of the stimulus and sensitivity to slow calcium buffers reported for asynchronous release. The neuromuscular junction and CNS neurons share these features, raising the possibility that secondary calcium sources are common among synapses with prominent asynchronous release. |
| first_indexed | 2024-04-12T01:59:00Z |
| format | Article |
| id | doaj.art-3edb93c0a6af4363aaf060e34bd4a9ca |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T01:59:00Z |
| publishDate | 2013-12-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-3edb93c0a6af4363aaf060e34bd4a9ca2022-12-22T03:52:43ZengeLife Sciences Publications LtdeLife2050-084X2013-12-01210.7554/eLife.01206Synchronous and asynchronous modes of synaptic transmission utilize different calcium sourcesHua Wen0Jeffrey M Hubbard1Benjamin Rakela2Michael W Linhoff3Gail Mandel4Paul Brehm5Vollum Institute, Oregon Health and Science University, Portland, United StatesVollum Institute, Oregon Health and Science University, Portland, United StatesVollum Institute, Oregon Health and Science University, Portland, United StatesVollum Institute, Oregon Health and Science University, Portland, United StatesVollum Institute, Oregon Health and Science University, Portland, United States; Howard Hughes Medical Institute, Oregon Health and Science University, Portland, United StatesVollum Institute, Oregon Health and Science University, Portland, United StatesAsynchronous transmission plays a prominent role at certain synapses but lacks the mechanistic insights of its synchronous counterpart. The current view posits that triggering of asynchronous release during repetitive stimulation involves expansion of the same calcium domains underlying synchronous transmission. In this study, live imaging and paired patch clamp recording at the zebrafish neuromuscular synapse reveal contributions by spatially distinct calcium sources. Synchronous release is tied to calcium entry into synaptic boutons via P/Q type calcium channels, whereas asynchronous release is boosted by a propagating intracellular calcium source initiated at off-synaptic locations in the axon and axonal branch points. This secondary calcium source fully accounts for the persistence following termination of the stimulus and sensitivity to slow calcium buffers reported for asynchronous release. The neuromuscular junction and CNS neurons share these features, raising the possibility that secondary calcium sources are common among synapses with prominent asynchronous release.https://elifesciences.org/articles/01206synaptopHluorincalcium indicatormotor neuron |
| spellingShingle | Hua Wen Jeffrey M Hubbard Benjamin Rakela Michael W Linhoff Gail Mandel Paul Brehm Synchronous and asynchronous modes of synaptic transmission utilize different calcium sources eLife synaptopHluorin calcium indicator motor neuron |
| title | Synchronous and asynchronous modes of synaptic transmission utilize different calcium sources |
| title_full | Synchronous and asynchronous modes of synaptic transmission utilize different calcium sources |
| title_fullStr | Synchronous and asynchronous modes of synaptic transmission utilize different calcium sources |
| title_full_unstemmed | Synchronous and asynchronous modes of synaptic transmission utilize different calcium sources |
| title_short | Synchronous and asynchronous modes of synaptic transmission utilize different calcium sources |
| title_sort | synchronous and asynchronous modes of synaptic transmission utilize different calcium sources |
| topic | synaptopHluorin calcium indicator motor neuron |
| url | https://elifesciences.org/articles/01206 |
| work_keys_str_mv | AT huawen synchronousandasynchronousmodesofsynaptictransmissionutilizedifferentcalciumsources AT jeffreymhubbard synchronousandasynchronousmodesofsynaptictransmissionutilizedifferentcalciumsources AT benjaminrakela synchronousandasynchronousmodesofsynaptictransmissionutilizedifferentcalciumsources AT michaelwlinhoff synchronousandasynchronousmodesofsynaptictransmissionutilizedifferentcalciumsources AT gailmandel synchronousandasynchronousmodesofsynaptictransmissionutilizedifferentcalciumsources AT paulbrehm synchronousandasynchronousmodesofsynaptictransmissionutilizedifferentcalciumsources |