Deoxynivalenol and Zearalenone: Different Mycotoxins with Different Toxic Effects in the Sertoli Cells of <i>Equus asinus</i>
(1) Background: Deoxynivalenol (DON) and zearalenone (ZEA) are type B trichothecene mycotoxins that exert serious toxic effects on the reproduction of domestic animals. However, there is little information about the toxicity of mycotoxins on testis development in <i>Equus asinus</i>. Thi...
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MDPI AG
2021-07-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/10/8/1898 |
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| author | Jun-Lin Song Guo-Liang Zhang |
| author_facet | Jun-Lin Song Guo-Liang Zhang |
| author_sort | Jun-Lin Song |
| collection | DOAJ |
| description | (1) Background: Deoxynivalenol (DON) and zearalenone (ZEA) are type B trichothecene mycotoxins that exert serious toxic effects on the reproduction of domestic animals. However, there is little information about the toxicity of mycotoxins on testis development in <i>Equus asinus</i>. This study investigated the biological effects of DON and ZEA exposure on Sertoli cells (SCs) of <i>Equus asinus</i>; (2) Methods: We administered 10 μM and 30 μM DON and ZEA to cells cultured in vitro; (3) Results: The results showed that 10 μM DON exposure remarkably changed pyroptosis-associated genes and that 30 μM ZEA exposure changed inflammation-associated genes in SCs. The mRNA expression of cancer-promoting genes was remarkably upregulated in the cells exposed to DON or 30 μM ZEA; in particular, DON and ZEA remarkably disturbed the expression of androgen and oestrogen secretion-related genes. Furthermore, quantitative RT-PCR, Western blot, and immunofluorescence analyses verified the different expression patterns of related genes in DON- and ZEA-exposed SCs; (4) Conclusions: Collectively, these results illustrated the impact of exposure to different toxins and concrete toxicity on the mRNA expression of SCs from <i>Equus asinus</i> in vitro. |
| first_indexed | 2024-03-10T08:55:23Z |
| format | Article |
| id | doaj.art-3edf695ba2ea4e599f984a6a392a23da |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T08:55:23Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-3edf695ba2ea4e599f984a6a392a23da2023-11-22T07:08:53ZengMDPI AGCells2073-44092021-07-01108189810.3390/cells10081898Deoxynivalenol and Zearalenone: Different Mycotoxins with Different Toxic Effects in the Sertoli Cells of <i>Equus asinus</i>Jun-Lin Song0Guo-Liang Zhang1College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China(1) Background: Deoxynivalenol (DON) and zearalenone (ZEA) are type B trichothecene mycotoxins that exert serious toxic effects on the reproduction of domestic animals. However, there is little information about the toxicity of mycotoxins on testis development in <i>Equus asinus</i>. This study investigated the biological effects of DON and ZEA exposure on Sertoli cells (SCs) of <i>Equus asinus</i>; (2) Methods: We administered 10 μM and 30 μM DON and ZEA to cells cultured in vitro; (3) Results: The results showed that 10 μM DON exposure remarkably changed pyroptosis-associated genes and that 30 μM ZEA exposure changed inflammation-associated genes in SCs. The mRNA expression of cancer-promoting genes was remarkably upregulated in the cells exposed to DON or 30 μM ZEA; in particular, DON and ZEA remarkably disturbed the expression of androgen and oestrogen secretion-related genes. Furthermore, quantitative RT-PCR, Western blot, and immunofluorescence analyses verified the different expression patterns of related genes in DON- and ZEA-exposed SCs; (4) Conclusions: Collectively, these results illustrated the impact of exposure to different toxins and concrete toxicity on the mRNA expression of SCs from <i>Equus asinus</i> in vitro.https://www.mdpi.com/2073-4409/10/8/1898DONZEA<i>Equus asinus</i>Sertoli cellspyroptosisRNA-seq |
| spellingShingle | Jun-Lin Song Guo-Liang Zhang Deoxynivalenol and Zearalenone: Different Mycotoxins with Different Toxic Effects in the Sertoli Cells of <i>Equus asinus</i> Cells DON ZEA <i>Equus asinus</i> Sertoli cells pyroptosis RNA-seq |
| title | Deoxynivalenol and Zearalenone: Different Mycotoxins with Different Toxic Effects in the Sertoli Cells of <i>Equus asinus</i> |
| title_full | Deoxynivalenol and Zearalenone: Different Mycotoxins with Different Toxic Effects in the Sertoli Cells of <i>Equus asinus</i> |
| title_fullStr | Deoxynivalenol and Zearalenone: Different Mycotoxins with Different Toxic Effects in the Sertoli Cells of <i>Equus asinus</i> |
| title_full_unstemmed | Deoxynivalenol and Zearalenone: Different Mycotoxins with Different Toxic Effects in the Sertoli Cells of <i>Equus asinus</i> |
| title_short | Deoxynivalenol and Zearalenone: Different Mycotoxins with Different Toxic Effects in the Sertoli Cells of <i>Equus asinus</i> |
| title_sort | deoxynivalenol and zearalenone different mycotoxins with different toxic effects in the sertoli cells of i equus asinus i |
| topic | DON ZEA <i>Equus asinus</i> Sertoli cells pyroptosis RNA-seq |
| url | https://www.mdpi.com/2073-4409/10/8/1898 |
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