Rapid Screening and Identification of BSA Bound Ligands from Radix astragali Using BSA Immobilized Magnetic Nanoparticles Coupled with HPLC-MS
Radix astragali is widely used either as a single herb or as a collection of herbs in a complex prescription in China. In this study, bovine serum albumin functionalized magnetic nanoparticles (BSA-MN) coupled with high performance liquid chromatography-mass spectrometry (HPLC-MS) were used to scree...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2016-11-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1420-3049/21/11/1471 |
| _version_ | 1811324616008794112 |
|---|---|
| author | Liangliang Liu Juan Leng Xiai Yang Liping Liao Yin Cen Aiping Xiao Lei Ma |
| author_facet | Liangliang Liu Juan Leng Xiai Yang Liping Liao Yin Cen Aiping Xiao Lei Ma |
| author_sort | Liangliang Liu |
| collection | DOAJ |
| description | Radix astragali is widely used either as a single herb or as a collection of herbs in a complex prescription in China. In this study, bovine serum albumin functionalized magnetic nanoparticles (BSA-MN) coupled with high performance liquid chromatography-mass spectrometry (HPLC-MS) were used to screen and identify bound ligands from the n-butanol part of a Radix astragali extract. The prepared BSA-MN showed sufficient magnetic response for the separation with an ordinary magnet and satisfied reusability. Fundamental parameters affecting the preparation of BSA-MN and the screening efficiency were studied and optimized. Under the optimum conditions, four bound ligands were screened out from the n-butanol part of a Radix astragali extract and identified as genistin (1), calycosin-7-O-β-d-glucoside (2), ononin (3) and formononetin (4). This effective method could be widely applied for rapid screening and identification of active compounds from complex mixtures without the need for preparative isolation. |
| first_indexed | 2024-04-13T14:17:21Z |
| format | Article |
| id | doaj.art-3ee008c3cb8248b688da49ce14217034 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-13T14:17:21Z |
| publishDate | 2016-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-3ee008c3cb8248b688da49ce142170342022-12-22T02:43:35ZengMDPI AGMolecules1420-30492016-11-012111147110.3390/molecules21111471molecules21111471Rapid Screening and Identification of BSA Bound Ligands from Radix astragali Using BSA Immobilized Magnetic Nanoparticles Coupled with HPLC-MSLiangliang Liu0Juan Leng1Xiai Yang2Liping Liao3Yin Cen4Aiping Xiao5Lei Ma6Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, ChinaInstitute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, ChinaInstitute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, ChinaInstitute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, ChinaCollege of Chemistry and Chemical Engineering, Central South University, Changsha 410083, ChinaInstitute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang 455000, ChinaRadix astragali is widely used either as a single herb or as a collection of herbs in a complex prescription in China. In this study, bovine serum albumin functionalized magnetic nanoparticles (BSA-MN) coupled with high performance liquid chromatography-mass spectrometry (HPLC-MS) were used to screen and identify bound ligands from the n-butanol part of a Radix astragali extract. The prepared BSA-MN showed sufficient magnetic response for the separation with an ordinary magnet and satisfied reusability. Fundamental parameters affecting the preparation of BSA-MN and the screening efficiency were studied and optimized. Under the optimum conditions, four bound ligands were screened out from the n-butanol part of a Radix astragali extract and identified as genistin (1), calycosin-7-O-β-d-glucoside (2), ononin (3) and formononetin (4). This effective method could be widely applied for rapid screening and identification of active compounds from complex mixtures without the need for preparative isolation.http://www.mdpi.com/1420-3049/21/11/1471bovine serum albuminHPLC-MSligandsmagnetic nanoparticlesRadix astragali |
| spellingShingle | Liangliang Liu Juan Leng Xiai Yang Liping Liao Yin Cen Aiping Xiao Lei Ma Rapid Screening and Identification of BSA Bound Ligands from Radix astragali Using BSA Immobilized Magnetic Nanoparticles Coupled with HPLC-MS Molecules bovine serum albumin HPLC-MS ligands magnetic nanoparticles Radix astragali |
| title | Rapid Screening and Identification of BSA Bound Ligands from Radix astragali Using BSA Immobilized Magnetic Nanoparticles Coupled with HPLC-MS |
| title_full | Rapid Screening and Identification of BSA Bound Ligands from Radix astragali Using BSA Immobilized Magnetic Nanoparticles Coupled with HPLC-MS |
| title_fullStr | Rapid Screening and Identification of BSA Bound Ligands from Radix astragali Using BSA Immobilized Magnetic Nanoparticles Coupled with HPLC-MS |
| title_full_unstemmed | Rapid Screening and Identification of BSA Bound Ligands from Radix astragali Using BSA Immobilized Magnetic Nanoparticles Coupled with HPLC-MS |
| title_short | Rapid Screening and Identification of BSA Bound Ligands from Radix astragali Using BSA Immobilized Magnetic Nanoparticles Coupled with HPLC-MS |
| title_sort | rapid screening and identification of bsa bound ligands from radix astragali using bsa immobilized magnetic nanoparticles coupled with hplc ms |
| topic | bovine serum albumin HPLC-MS ligands magnetic nanoparticles Radix astragali |
| url | http://www.mdpi.com/1420-3049/21/11/1471 |
| work_keys_str_mv | AT liangliangliu rapidscreeningandidentificationofbsaboundligandsfromradixastragaliusingbsaimmobilizedmagneticnanoparticlescoupledwithhplcms AT juanleng rapidscreeningandidentificationofbsaboundligandsfromradixastragaliusingbsaimmobilizedmagneticnanoparticlescoupledwithhplcms AT xiaiyang rapidscreeningandidentificationofbsaboundligandsfromradixastragaliusingbsaimmobilizedmagneticnanoparticlescoupledwithhplcms AT lipingliao rapidscreeningandidentificationofbsaboundligandsfromradixastragaliusingbsaimmobilizedmagneticnanoparticlescoupledwithhplcms AT yincen rapidscreeningandidentificationofbsaboundligandsfromradixastragaliusingbsaimmobilizedmagneticnanoparticlescoupledwithhplcms AT aipingxiao rapidscreeningandidentificationofbsaboundligandsfromradixastragaliusingbsaimmobilizedmagneticnanoparticlescoupledwithhplcms AT leima rapidscreeningandidentificationofbsaboundligandsfromradixastragaliusingbsaimmobilizedmagneticnanoparticlescoupledwithhplcms |