An abortion storm in a goat farm in the Northeast Region of Brazil was caused by the atypical Toxoplasma gondii genotype #13
The objective of this study was to characterise a Toxoplasma gondii-induced abortion outbreak on a goat farm in the State of Paraíba, Northeast Region of Brazil. From a herd of 10 does, seven experienced abortions and one gave birth to twins (one stillborn and the other weak and underdeveloped). Ser...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-01-01
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| Series: | Current Research in Parasitology and Vector-Borne Diseases |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667114X23000456 |
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| author | Vinícius Longo Ribeiro Vilela Thais Ferreira Feitosa Sara Vilar Dantas Simões Rinaldo Aparecido Mota Frank Katzer Paul M. Bartley |
| author_facet | Vinícius Longo Ribeiro Vilela Thais Ferreira Feitosa Sara Vilar Dantas Simões Rinaldo Aparecido Mota Frank Katzer Paul M. Bartley |
| author_sort | Vinícius Longo Ribeiro Vilela |
| collection | DOAJ |
| description | The objective of this study was to characterise a Toxoplasma gondii-induced abortion outbreak on a goat farm in the State of Paraíba, Northeast Region of Brazil. From a herd of 10 does, seven experienced abortions and one gave birth to twins (one stillborn and the other weak and underdeveloped). Serum samples from all of the does were analysed by indirect fluorescent antibody test (IFAT). Samples of colostrum and placenta from two does, along with lung, heart, brain and umbilical cord samples from four of the foetuses, were screened by nested ITS1 PCR specific for T. gondii. The positive samples were then analysed by multiplex nested PCR-RFLP. All ten does tested positive by IFAT for anti-T. gondii IgG (titrations ranging from 1:4096 to 1:65,536). The ITS1 PCR screening revealed T. gondii DNA in the placenta (2/2), colostrum (2/2), umbilical cord (2/4), lung (1/4), heart (1/4), and brain (1/4). Four samples produced complete RFLP genotyping results, identifying a single genotype, ToxoDB #13. In conclusion, we demonstrated a high rate of abortion caused by T. gondii in a goat herd, highlighting the pathogenicity of genotype #13, one of the most prevalent genotypes of T. gondii in Brazil. |
| first_indexed | 2024-03-08T18:59:43Z |
| format | Article |
| id | doaj.art-3ee0de302b9d4f658822666b18e5352a |
| institution | Directory Open Access Journal |
| issn | 2667-114X |
| language | English |
| last_indexed | 2024-03-08T18:59:43Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Research in Parasitology and Vector-Borne Diseases |
| spelling | doaj.art-3ee0de302b9d4f658822666b18e5352a2023-12-28T05:20:05ZengElsevierCurrent Research in Parasitology and Vector-Borne Diseases2667-114X2024-01-015100157An abortion storm in a goat farm in the Northeast Region of Brazil was caused by the atypical Toxoplasma gondii genotype #13Vinícius Longo Ribeiro Vilela0Thais Ferreira Feitosa1Sara Vilar Dantas Simões2Rinaldo Aparecido Mota3Frank Katzer4Paul M. Bartley5Department of Veterinary Medicine, Instituto Federal da Paraíba - IFPB, Sousa, Paraíba ZC 58800-970, Brazil; Corresponding author.Department of Veterinary Medicine, Instituto Federal da Paraíba - IFPB, Sousa, Paraíba ZC 58800-970, BrazilDepartment of Veterinary Sciences, Universidade Federal da Paraíba – UFPB, Areia, Paraíba ZC 58397-000, BrazilDepartment of Veterinary Medicine, Universidade Federal Rural de Pernambuco – UFRPE, Recife, Pernambuco ZC 52171-900, BrazilMoredun Research Institute, Pentlands Science Park, Penicuik EH26 0PZ, Scotland, United KingdomMoredun Research Institute, Pentlands Science Park, Penicuik EH26 0PZ, Scotland, United KingdomThe objective of this study was to characterise a Toxoplasma gondii-induced abortion outbreak on a goat farm in the State of Paraíba, Northeast Region of Brazil. From a herd of 10 does, seven experienced abortions and one gave birth to twins (one stillborn and the other weak and underdeveloped). Serum samples from all of the does were analysed by indirect fluorescent antibody test (IFAT). Samples of colostrum and placenta from two does, along with lung, heart, brain and umbilical cord samples from four of the foetuses, were screened by nested ITS1 PCR specific for T. gondii. The positive samples were then analysed by multiplex nested PCR-RFLP. All ten does tested positive by IFAT for anti-T. gondii IgG (titrations ranging from 1:4096 to 1:65,536). The ITS1 PCR screening revealed T. gondii DNA in the placenta (2/2), colostrum (2/2), umbilical cord (2/4), lung (1/4), heart (1/4), and brain (1/4). Four samples produced complete RFLP genotyping results, identifying a single genotype, ToxoDB #13. In conclusion, we demonstrated a high rate of abortion caused by T. gondii in a goat herd, highlighting the pathogenicity of genotype #13, one of the most prevalent genotypes of T. gondii in Brazil.http://www.sciencedirect.com/science/article/pii/S2667114X23000456IFATPCR-RFLPOutbreakToxoplasmosisVertical transmission |
| spellingShingle | Vinícius Longo Ribeiro Vilela Thais Ferreira Feitosa Sara Vilar Dantas Simões Rinaldo Aparecido Mota Frank Katzer Paul M. Bartley An abortion storm in a goat farm in the Northeast Region of Brazil was caused by the atypical Toxoplasma gondii genotype #13 Current Research in Parasitology and Vector-Borne Diseases IFAT PCR-RFLP Outbreak Toxoplasmosis Vertical transmission |
| title | An abortion storm in a goat farm in the Northeast Region of Brazil was caused by the atypical Toxoplasma gondii genotype #13 |
| title_full | An abortion storm in a goat farm in the Northeast Region of Brazil was caused by the atypical Toxoplasma gondii genotype #13 |
| title_fullStr | An abortion storm in a goat farm in the Northeast Region of Brazil was caused by the atypical Toxoplasma gondii genotype #13 |
| title_full_unstemmed | An abortion storm in a goat farm in the Northeast Region of Brazil was caused by the atypical Toxoplasma gondii genotype #13 |
| title_short | An abortion storm in a goat farm in the Northeast Region of Brazil was caused by the atypical Toxoplasma gondii genotype #13 |
| title_sort | abortion storm in a goat farm in the northeast region of brazil was caused by the atypical toxoplasma gondii genotype 13 |
| topic | IFAT PCR-RFLP Outbreak Toxoplasmosis Vertical transmission |
| url | http://www.sciencedirect.com/science/article/pii/S2667114X23000456 |
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