Rapid sensitive validated UPLCâMS method for determination of venlafaxine and its metabolite in rat plasma: Application to pharmacokinetic study
A new ultra-performance liquid chromatographyâelectrospray ionization mass spectrometry (UPLCâMS/ESI) method for simultaneous determination of venlafaxine (VEN) and its metabolite O-desmethylvenlafaxine (ODV) in rat plasma has been developed and validated using Venlafaxine d6 as the internal standar...
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| Format: | Article |
| Language: | English |
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Elsevier
2013-12-01
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| Series: | Journal of Pharmaceutical Analysis |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095177913000567 |
| _version_ | 1818727328908312576 |
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| author | Sunil Kumar Dubey R.N. Saha Hemanth Jangala S. Pasha |
| author_facet | Sunil Kumar Dubey R.N. Saha Hemanth Jangala S. Pasha |
| author_sort | Sunil Kumar Dubey |
| collection | DOAJ |
| description | A new ultra-performance liquid chromatographyâelectrospray ionization mass spectrometry (UPLCâMS/ESI) method for simultaneous determination of venlafaxine (VEN) and its metabolite O-desmethylvenlafaxine (ODV) in rat plasma has been developed and validated using Venlafaxine d6 as the internal standard. The compounds and internal standard were extracted from plasma by solid phase extraction. The UPLC separation of the analytes was performed on ACQUITY UPLC® BEH Shield RP18 (1.7 µm, 100 mmÃ2.1 mm) column, using isocratic elution with mobile phase constituted of water (containing 2 mM ammonium acetate): acetonitrile (20:80, v/v) at a flow rate of 0.3 mL/min. All of the analytes were eluted within 1.5 min. The compounds were ionized in the electrospray ionization (ESI) ion source of the mass spectrometer, operating in multiple reaction monitoring (MRM) and positive ion mode. The precursor to product ion transitions monitored for VEN, ODV and Venlafaxine d6 were m/z 278.3â121.08, 264.2â107.1 and 284.4â121.0, respectively. The developed and validated method was used for the pharmacokinetic study of VEN in rats. Keywords: Venlafaxine, O-desmethylvenlafaxine, Metabolite, UPLCâMS/ES |
| first_indexed | 2024-12-17T22:12:22Z |
| format | Article |
| id | doaj.art-3ee202b9286b409aaa28f62554f5d33c |
| institution | Directory Open Access Journal |
| issn | 2095-1779 |
| language | English |
| last_indexed | 2024-12-17T22:12:22Z |
| publishDate | 2013-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pharmaceutical Analysis |
| spelling | doaj.art-3ee202b9286b409aaa28f62554f5d33c2022-12-21T21:30:42ZengElsevierJournal of Pharmaceutical Analysis2095-17792013-12-0136466471Rapid sensitive validated UPLCâMS method for determination of venlafaxine and its metabolite in rat plasma: Application to pharmacokinetic studySunil Kumar Dubey0R.N. Saha1Hemanth Jangala2S. Pasha3Department of Pharmacy, Birla Institute of Technology & Science, Pilani, India; Corresponding author. Tel.: +91 8239703734.Department of Pharmacy, Birla Institute of Technology & Science, Pilani, IndiaDepartment of Pharmacy, Birla Institute of Technology & Science, Pilani, IndiaInstitute of Genomics and Integrative Biology, Mall Road, Delhi, IndiaA new ultra-performance liquid chromatographyâelectrospray ionization mass spectrometry (UPLCâMS/ESI) method for simultaneous determination of venlafaxine (VEN) and its metabolite O-desmethylvenlafaxine (ODV) in rat plasma has been developed and validated using Venlafaxine d6 as the internal standard. The compounds and internal standard were extracted from plasma by solid phase extraction. The UPLC separation of the analytes was performed on ACQUITY UPLC® BEH Shield RP18 (1.7 µm, 100 mmÃ2.1 mm) column, using isocratic elution with mobile phase constituted of water (containing 2 mM ammonium acetate): acetonitrile (20:80, v/v) at a flow rate of 0.3 mL/min. All of the analytes were eluted within 1.5 min. The compounds were ionized in the electrospray ionization (ESI) ion source of the mass spectrometer, operating in multiple reaction monitoring (MRM) and positive ion mode. The precursor to product ion transitions monitored for VEN, ODV and Venlafaxine d6 were m/z 278.3â121.08, 264.2â107.1 and 284.4â121.0, respectively. The developed and validated method was used for the pharmacokinetic study of VEN in rats. Keywords: Venlafaxine, O-desmethylvenlafaxine, Metabolite, UPLCâMS/EShttp://www.sciencedirect.com/science/article/pii/S2095177913000567 |
| spellingShingle | Sunil Kumar Dubey R.N. Saha Hemanth Jangala S. Pasha Rapid sensitive validated UPLCâMS method for determination of venlafaxine and its metabolite in rat plasma: Application to pharmacokinetic study Journal of Pharmaceutical Analysis |
| title | Rapid sensitive validated UPLCâMS method for determination of venlafaxine and its metabolite in rat plasma: Application to pharmacokinetic study |
| title_full | Rapid sensitive validated UPLCâMS method for determination of venlafaxine and its metabolite in rat plasma: Application to pharmacokinetic study |
| title_fullStr | Rapid sensitive validated UPLCâMS method for determination of venlafaxine and its metabolite in rat plasma: Application to pharmacokinetic study |
| title_full_unstemmed | Rapid sensitive validated UPLCâMS method for determination of venlafaxine and its metabolite in rat plasma: Application to pharmacokinetic study |
| title_short | Rapid sensitive validated UPLCâMS method for determination of venlafaxine and its metabolite in rat plasma: Application to pharmacokinetic study |
| title_sort | rapid sensitive validated uplcams method for determination of venlafaxine and its metabolite in rat plasma application to pharmacokinetic study |
| url | http://www.sciencedirect.com/science/article/pii/S2095177913000567 |
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