Integrating Transcriptomics and Metabolomics to Explore the Novel Pathway of <i>Fusobacterium nucleatum</i> Invading Colon Cancer Cells
Colorectal cancer (CRC) is a malignancy with a very high incidence and mortality rate worldwide. <i>Fusobacterium nucleatum</i> bacteria and their metabolites play a role in inducing and promoting CRC; however, no studies on the exchange of information between <i>Fusobacterium nucl...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-01-01
|
| Series: | Pathogens |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-0817/12/2/201 |
| _version_ | 1797618875486961664 |
|---|---|
| author | Xinyu Wu Jinzhao Xu Xiaoying Yang Danping Wang Xiaoxi Xu |
| author_facet | Xinyu Wu Jinzhao Xu Xiaoying Yang Danping Wang Xiaoxi Xu |
| author_sort | Xinyu Wu |
| collection | DOAJ |
| description | Colorectal cancer (CRC) is a malignancy with a very high incidence and mortality rate worldwide. <i>Fusobacterium nucleatum</i> bacteria and their metabolites play a role in inducing and promoting CRC; however, no studies on the exchange of information between <i>Fusobacterium nucleatum</i> extracellular vesicles (<i>Fn</i>evs) and CRC cells have been reported. Our research shows that <i>Fusobacterium nucleatum</i> ATCC25586 secretes extracellular vesicles carrying active substances from parental bacteria which are endocytosed by colon cancer cells. Moreover, <i>Fn</i>evs promote the proliferation, migration, and invasion of CRC cells and inhibit apoptosis; they also improve the ability of CRC cells to resist oxidative stress and SOD enzyme activity. The genes differentially expressed after transcriptome sequencing are mostly involved in the positive regulation of tumor cell proliferation. After detecting differential metabolites using liquid chromatography–tandem mass spectrometry, <i>Fn</i>evs were found to promote cell proliferation by regulating amino acid biosynthesis in CRC cells and metabolic pathways such as central carbon metabolism, protein digestion, and uptake in cancer. In summary, this study not only found new evidence of the synergistic effect of pathogenic bacteria and colon cancer tumor cells, but also provides a new direction for the early diagnosis and targeted treatment of colon cancer. |
| first_indexed | 2024-03-11T08:18:46Z |
| format | Article |
| id | doaj.art-3ee78d6c92f94091bdc9acf403a41c30 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-11T08:18:46Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-3ee78d6c92f94091bdc9acf403a41c302023-11-16T22:33:24ZengMDPI AGPathogens2076-08172023-01-0112220110.3390/pathogens12020201Integrating Transcriptomics and Metabolomics to Explore the Novel Pathway of <i>Fusobacterium nucleatum</i> Invading Colon Cancer CellsXinyu Wu0Jinzhao Xu1Xiaoying Yang2Danping Wang3Xiaoxi Xu4Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, ChinaColorectal cancer (CRC) is a malignancy with a very high incidence and mortality rate worldwide. <i>Fusobacterium nucleatum</i> bacteria and their metabolites play a role in inducing and promoting CRC; however, no studies on the exchange of information between <i>Fusobacterium nucleatum</i> extracellular vesicles (<i>Fn</i>evs) and CRC cells have been reported. Our research shows that <i>Fusobacterium nucleatum</i> ATCC25586 secretes extracellular vesicles carrying active substances from parental bacteria which are endocytosed by colon cancer cells. Moreover, <i>Fn</i>evs promote the proliferation, migration, and invasion of CRC cells and inhibit apoptosis; they also improve the ability of CRC cells to resist oxidative stress and SOD enzyme activity. The genes differentially expressed after transcriptome sequencing are mostly involved in the positive regulation of tumor cell proliferation. After detecting differential metabolites using liquid chromatography–tandem mass spectrometry, <i>Fn</i>evs were found to promote cell proliferation by regulating amino acid biosynthesis in CRC cells and metabolic pathways such as central carbon metabolism, protein digestion, and uptake in cancer. In summary, this study not only found new evidence of the synergistic effect of pathogenic bacteria and colon cancer tumor cells, but also provides a new direction for the early diagnosis and targeted treatment of colon cancer.https://www.mdpi.com/2076-0817/12/2/201<i>Fusobacterium nucleatum</i>extracellular vesiclescolon cancer cellstranscriptomicsmetabolomics |
| spellingShingle | Xinyu Wu Jinzhao Xu Xiaoying Yang Danping Wang Xiaoxi Xu Integrating Transcriptomics and Metabolomics to Explore the Novel Pathway of <i>Fusobacterium nucleatum</i> Invading Colon Cancer Cells Pathogens <i>Fusobacterium nucleatum</i> extracellular vesicles colon cancer cells transcriptomics metabolomics |
| title | Integrating Transcriptomics and Metabolomics to Explore the Novel Pathway of <i>Fusobacterium nucleatum</i> Invading Colon Cancer Cells |
| title_full | Integrating Transcriptomics and Metabolomics to Explore the Novel Pathway of <i>Fusobacterium nucleatum</i> Invading Colon Cancer Cells |
| title_fullStr | Integrating Transcriptomics and Metabolomics to Explore the Novel Pathway of <i>Fusobacterium nucleatum</i> Invading Colon Cancer Cells |
| title_full_unstemmed | Integrating Transcriptomics and Metabolomics to Explore the Novel Pathway of <i>Fusobacterium nucleatum</i> Invading Colon Cancer Cells |
| title_short | Integrating Transcriptomics and Metabolomics to Explore the Novel Pathway of <i>Fusobacterium nucleatum</i> Invading Colon Cancer Cells |
| title_sort | integrating transcriptomics and metabolomics to explore the novel pathway of i fusobacterium nucleatum i invading colon cancer cells |
| topic | <i>Fusobacterium nucleatum</i> extracellular vesicles colon cancer cells transcriptomics metabolomics |
| url | https://www.mdpi.com/2076-0817/12/2/201 |
| work_keys_str_mv | AT xinyuwu integratingtranscriptomicsandmetabolomicstoexplorethenovelpathwayofifusobacteriumnucleatumiinvadingcoloncancercells AT jinzhaoxu integratingtranscriptomicsandmetabolomicstoexplorethenovelpathwayofifusobacteriumnucleatumiinvadingcoloncancercells AT xiaoyingyang integratingtranscriptomicsandmetabolomicstoexplorethenovelpathwayofifusobacteriumnucleatumiinvadingcoloncancercells AT danpingwang integratingtranscriptomicsandmetabolomicstoexplorethenovelpathwayofifusobacteriumnucleatumiinvadingcoloncancercells AT xiaoxixu integratingtranscriptomicsandmetabolomicstoexplorethenovelpathwayofifusobacteriumnucleatumiinvadingcoloncancercells |