Analysis of Raffinose Synthase Gene Family in Bread Wheat and Identification of Drought Resistance and Salt Tolerance Function of <i>TaRS15-3B</i>
Raffinose synthase (RS) plays a crucial role in plant growth and development, as well as in responses to biotic stresses and abiotic stresses, yet few studies have been conducted on its role in bread wheat. Therefore, in this study we screened and identified a family of bread wheat raffinose synthas...
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MDPI AG
2023-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/13/11185 |
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| author | Jiagui Guo Yan Yang Tingting Wang Yizhen Wang Xin Zhang Donghong Min Xiaohong Zhang |
| author_facet | Jiagui Guo Yan Yang Tingting Wang Yizhen Wang Xin Zhang Donghong Min Xiaohong Zhang |
| author_sort | Jiagui Guo |
| collection | DOAJ |
| description | Raffinose synthase (RS) plays a crucial role in plant growth and development, as well as in responses to biotic stresses and abiotic stresses, yet few studies have been conducted on its role in bread wheat. Therefore, in this study we screened and identified a family of bread wheat raffinose synthase genes based on bread wheat genome information and analyzed their physicochemical properties, phylogenetic evolutionary relationships, conserved structural domains, promoter cis-acting elements, and expression patterns. The BSMV-induced silencing of <i>TaRS15-3B</i> resulted in the bread wheat seedlings being susceptible to drought and salt stress and reduced the expression levels of stress-related and ROS-scavenging genes in bread wheat plants. This further affected the ability of bread wheat to cope with drought and salt stress. In conclusion, this study revealed that the RS gene family in bread wheat plays an important role in plant response to abiotic stresses and that the <i>TaRS15-3B</i> gene can improve the tolerance of transgenic bread wheat to drought and salt stresses, provide directions for the study of other RS gene families in bread wheat, and supply candidate genes for use in molecular breeding of bread wheat for stress resistance. |
| first_indexed | 2024-03-11T01:37:57Z |
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| id | doaj.art-3ee7df96161440e0b29a7397938662e4 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T01:37:57Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-3ee7df96161440e0b29a7397938662e42023-11-18T16:49:23ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-07-0124131118510.3390/ijms241311185Analysis of Raffinose Synthase Gene Family in Bread Wheat and Identification of Drought Resistance and Salt Tolerance Function of <i>TaRS15-3B</i>Jiagui Guo0Yan Yang1Tingting Wang2Yizhen Wang3Xin Zhang4Donghong Min5Xiaohong Zhang6State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Sciences, Northwest A&F University, Xianyang 712100, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Xianyang 712100, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Sciences, Northwest A&F University, Xianyang 712100, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Sciences, Northwest A&F University, Xianyang 712100, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Sciences, Northwest A&F University, Xianyang 712100, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Xianyang 712100, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Sciences, Northwest A&F University, Xianyang 712100, ChinaRaffinose synthase (RS) plays a crucial role in plant growth and development, as well as in responses to biotic stresses and abiotic stresses, yet few studies have been conducted on its role in bread wheat. Therefore, in this study we screened and identified a family of bread wheat raffinose synthase genes based on bread wheat genome information and analyzed their physicochemical properties, phylogenetic evolutionary relationships, conserved structural domains, promoter cis-acting elements, and expression patterns. The BSMV-induced silencing of <i>TaRS15-3B</i> resulted in the bread wheat seedlings being susceptible to drought and salt stress and reduced the expression levels of stress-related and ROS-scavenging genes in bread wheat plants. This further affected the ability of bread wheat to cope with drought and salt stress. In conclusion, this study revealed that the RS gene family in bread wheat plays an important role in plant response to abiotic stresses and that the <i>TaRS15-3B</i> gene can improve the tolerance of transgenic bread wheat to drought and salt stresses, provide directions for the study of other RS gene families in bread wheat, and supply candidate genes for use in molecular breeding of bread wheat for stress resistance.https://www.mdpi.com/1422-0067/24/13/11185bread wheatraffinose synthase<i>TaRS15-3B</i>drought tolerancesalt tolerance |
| spellingShingle | Jiagui Guo Yan Yang Tingting Wang Yizhen Wang Xin Zhang Donghong Min Xiaohong Zhang Analysis of Raffinose Synthase Gene Family in Bread Wheat and Identification of Drought Resistance and Salt Tolerance Function of <i>TaRS15-3B</i> International Journal of Molecular Sciences bread wheat raffinose synthase <i>TaRS15-3B</i> drought tolerance salt tolerance |
| title | Analysis of Raffinose Synthase Gene Family in Bread Wheat and Identification of Drought Resistance and Salt Tolerance Function of <i>TaRS15-3B</i> |
| title_full | Analysis of Raffinose Synthase Gene Family in Bread Wheat and Identification of Drought Resistance and Salt Tolerance Function of <i>TaRS15-3B</i> |
| title_fullStr | Analysis of Raffinose Synthase Gene Family in Bread Wheat and Identification of Drought Resistance and Salt Tolerance Function of <i>TaRS15-3B</i> |
| title_full_unstemmed | Analysis of Raffinose Synthase Gene Family in Bread Wheat and Identification of Drought Resistance and Salt Tolerance Function of <i>TaRS15-3B</i> |
| title_short | Analysis of Raffinose Synthase Gene Family in Bread Wheat and Identification of Drought Resistance and Salt Tolerance Function of <i>TaRS15-3B</i> |
| title_sort | analysis of raffinose synthase gene family in bread wheat and identification of drought resistance and salt tolerance function of i tars15 3b i |
| topic | bread wheat raffinose synthase <i>TaRS15-3B</i> drought tolerance salt tolerance |
| url | https://www.mdpi.com/1422-0067/24/13/11185 |
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