Antimicrobial Activity the Essential Oil from <i>Croton pluriglandulosus</i> Carn. Leaves against Microorganisms of Clinical Interest
Multiresistant pathogens pose a serious threat to human health. The genus <i>Candida</i> is one class of human pathogenic yeasts responsible for infections affecting healthy and immunocompromised patients. In this context, plant essential oils emerged as a future natural alternative to c...
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| Format: | Article |
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MDPI AG
2023-07-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/9/7/756 |
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| author | Rayara J. P. Carvalho Pedro F. N. Souza Ellen A. Malveira Nilton A. S. Neto Romério R. S. Silva Gabriel L. C. Melo Ayrles F. B. Silva Leandro B. Lima Cynthia C. de Albuquerque Rafael W. Bastos Gustavo H. Goldman Cleverson D. T. de Freitas |
| author_facet | Rayara J. P. Carvalho Pedro F. N. Souza Ellen A. Malveira Nilton A. S. Neto Romério R. S. Silva Gabriel L. C. Melo Ayrles F. B. Silva Leandro B. Lima Cynthia C. de Albuquerque Rafael W. Bastos Gustavo H. Goldman Cleverson D. T. de Freitas |
| author_sort | Rayara J. P. Carvalho |
| collection | DOAJ |
| description | Multiresistant pathogens pose a serious threat to human health. The genus <i>Candida</i> is one class of human pathogenic yeasts responsible for infections affecting healthy and immunocompromised patients. In this context, plant essential oils emerged as a future natural alternative to control the diseases caused by these pathogens. Based on that, the present study aimed to evaluate the antimicrobial potential of essential oil from <i>C. pluriglandulosus</i> and understand the mechanism of action. Here, it highlighted antimicrobial activity and the mechanisms of action of the essential oil extracted from <i>C. pluriglandulosus</i> Carn.-Torres & Riina (<i>CpEO</i>) leaves on human pathogenic microorganisms in planktonic and biofilm lifestyles. In addition, for the first time, the oil composition was revealed by GC-MS analysis and the toxicity to human red blood cells (HRBC). Twenty-six chemical compounds were identified in <i>CpEO</i>, elemicin, bicyclogermacrene, caryophyllene, brevifolin, and 2,4,6-trimethoxy-styrene. Through hemolytic assay, it was shown that <i>CpEO</i> has no toxicity to human RBCs. At the concentration of 50 μg mL<sup>−1</sup>, <i>CpEO</i> did not show great antibacterial potential. However, promising data were found for <i>C. krusei</i> and <i>C. parapsilosis</i> inhibiting by 89.3% and 80.7% of planktonic cell growth and 83.5% and 77.9% the biofilm formation, respectively. Furthermore, the mechanisms of action <i>CpEO</i> were elucidated by fluorescence. Scanning electron microscopy revealed damage to the cell membrane and pore formation, ROS overproduction, and induction of apoptosis in candida cells. Our results reinforce the potential of <i>CpEO</i> as an effective alternative molecule of pharmaceutical interest. |
| first_indexed | 2024-03-11T00:55:33Z |
| format | Article |
| id | doaj.art-3eec04612a9741e1840eeb5cd00bdc2f |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-11T00:55:33Z |
| publishDate | 2023-07-01 |
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| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-3eec04612a9741e1840eeb5cd00bdc2f2023-11-18T20:01:59ZengMDPI AGJournal of Fungi2309-608X2023-07-019775610.3390/jof9070756Antimicrobial Activity the Essential Oil from <i>Croton pluriglandulosus</i> Carn. Leaves against Microorganisms of Clinical InterestRayara J. P. Carvalho0Pedro F. N. Souza1Ellen A. Malveira2Nilton A. S. Neto3Romério R. S. Silva4Gabriel L. C. Melo5Ayrles F. B. Silva6Leandro B. Lima7Cynthia C. de Albuquerque8Rafael W. Bastos9Gustavo H. Goldman10Cleverson D. T. de Freitas11Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza 60020-181, BrazilDepartment of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza 60020-181, BrazilDepartment of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza 60020-181, BrazilDepartment of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza 60020-181, BrazilDepartment of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza 60020-181, BrazilDepartment of Fishery Engineering, Federal University of Ceará, Fortaleza 60356-000, BrazilDepartment of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza 60020-181, BrazilDepartment of Chemistry, Faculty of Exact and Natural Sciences, State University of Rio Grande do Norte, Mossoró 59650-000, BrazilDepartment of Biological Sciences, Faculty of Exact and Natural Sciences, State University of Rio Grande do Norte, Mossoró 59650-000, BrazilDepartment of Microbiology and Parasitology, Biosciences Center, Federal University of Rio Grande do Norte, Natal 59078-970, BrazilFaculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, São Paulo 14040-903, BrazilDepartment of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza 60020-181, BrazilMultiresistant pathogens pose a serious threat to human health. The genus <i>Candida</i> is one class of human pathogenic yeasts responsible for infections affecting healthy and immunocompromised patients. In this context, plant essential oils emerged as a future natural alternative to control the diseases caused by these pathogens. Based on that, the present study aimed to evaluate the antimicrobial potential of essential oil from <i>C. pluriglandulosus</i> and understand the mechanism of action. Here, it highlighted antimicrobial activity and the mechanisms of action of the essential oil extracted from <i>C. pluriglandulosus</i> Carn.-Torres & Riina (<i>CpEO</i>) leaves on human pathogenic microorganisms in planktonic and biofilm lifestyles. In addition, for the first time, the oil composition was revealed by GC-MS analysis and the toxicity to human red blood cells (HRBC). Twenty-six chemical compounds were identified in <i>CpEO</i>, elemicin, bicyclogermacrene, caryophyllene, brevifolin, and 2,4,6-trimethoxy-styrene. Through hemolytic assay, it was shown that <i>CpEO</i> has no toxicity to human RBCs. At the concentration of 50 μg mL<sup>−1</sup>, <i>CpEO</i> did not show great antibacterial potential. However, promising data were found for <i>C. krusei</i> and <i>C. parapsilosis</i> inhibiting by 89.3% and 80.7% of planktonic cell growth and 83.5% and 77.9% the biofilm formation, respectively. Furthermore, the mechanisms of action <i>CpEO</i> were elucidated by fluorescence. Scanning electron microscopy revealed damage to the cell membrane and pore formation, ROS overproduction, and induction of apoptosis in candida cells. Our results reinforce the potential of <i>CpEO</i> as an effective alternative molecule of pharmaceutical interest.https://www.mdpi.com/2309-608X/9/7/756<i>Croton</i>yeastinhibitionchemical compositionaction mechanisms |
| spellingShingle | Rayara J. P. Carvalho Pedro F. N. Souza Ellen A. Malveira Nilton A. S. Neto Romério R. S. Silva Gabriel L. C. Melo Ayrles F. B. Silva Leandro B. Lima Cynthia C. de Albuquerque Rafael W. Bastos Gustavo H. Goldman Cleverson D. T. de Freitas Antimicrobial Activity the Essential Oil from <i>Croton pluriglandulosus</i> Carn. Leaves against Microorganisms of Clinical Interest Journal of Fungi <i>Croton</i> yeast inhibition chemical composition action mechanisms |
| title | Antimicrobial Activity the Essential Oil from <i>Croton pluriglandulosus</i> Carn. Leaves against Microorganisms of Clinical Interest |
| title_full | Antimicrobial Activity the Essential Oil from <i>Croton pluriglandulosus</i> Carn. Leaves against Microorganisms of Clinical Interest |
| title_fullStr | Antimicrobial Activity the Essential Oil from <i>Croton pluriglandulosus</i> Carn. Leaves against Microorganisms of Clinical Interest |
| title_full_unstemmed | Antimicrobial Activity the Essential Oil from <i>Croton pluriglandulosus</i> Carn. Leaves against Microorganisms of Clinical Interest |
| title_short | Antimicrobial Activity the Essential Oil from <i>Croton pluriglandulosus</i> Carn. Leaves against Microorganisms of Clinical Interest |
| title_sort | antimicrobial activity the essential oil from i croton pluriglandulosus i carn leaves against microorganisms of clinical interest |
| topic | <i>Croton</i> yeast inhibition chemical composition action mechanisms |
| url | https://www.mdpi.com/2309-608X/9/7/756 |
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