Phytophthora infestans Sporangia Produced in Artificial Media and Plant Lesions Have Subtly Divergent Transcription Profiles but Equivalent Infection Potential and Aggressiveness
Sporangia of the potato late blight agent Phytophthora infestans are often used in studies of pathogen biology and plant responses to infection. Investigations of spore biology can be challenging in oomycetes because their sporangia are physiologically active and change in response to environmental...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The American Phytopathological Society
2019-09-01
|
| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-12-18-0349-TA |
| _version_ | 1811264501716090880 |
|---|---|
| author | Wiphawee Leesutthiphonchai Howard S. Judelson |
| author_facet | Wiphawee Leesutthiphonchai Howard S. Judelson |
| author_sort | Wiphawee Leesutthiphonchai |
| collection | DOAJ |
| description | Sporangia of the potato late blight agent Phytophthora infestans are often used in studies of pathogen biology and plant responses to infection. Investigations of spore biology can be challenging in oomycetes because their sporangia are physiologically active and change in response to environmental factors and aging. Whether sporangia from artificial media and plant lesions are functionally equivalent has been a topic of debate. To address these issues, we compared the transcriptomes and infection ability of sporangia from rye-sucrose media, potato and tomato leaflets, and potato tubers. Small differences were observed between the mRNA profiles of sporangia from all sources, including variation in genes encoding metabolic enzymes, cell-wall-degrading enzymes, and ABC transporters. Small differences in sporangia age also resulted in variation in the transcriptome. Taking care to use sporangia of similar maturity, we observed that those sourced from media or plant lesions had similar rates of zoospore release and cyst germination. There were also no differences in infection rates or aggressiveness on leaflets, based on single-spore inoculation assays. Such results are discordant with those of a recent publication in this journal. Nevertheless, we conclude that sporangia from plant and media cultures are functionally similar and emphasize the importance of using “best practices” in experiments with sporangia to obtain reliable results. |
| first_indexed | 2024-04-12T20:04:26Z |
| format | Article |
| id | doaj.art-3eed4324b4d1496ab989fa11b73e6717 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-12T20:04:26Z |
| publishDate | 2019-09-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-3eed4324b4d1496ab989fa11b73e67172022-12-22T03:18:25ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062019-09-013291077108710.1094/MPMI-12-18-0349-TAPhytophthora infestans Sporangia Produced in Artificial Media and Plant Lesions Have Subtly Divergent Transcription Profiles but Equivalent Infection Potential and AggressivenessWiphawee Leesutthiphonchai0Howard S. Judelson1Department of Microbiology and Plant Pathology, University of California, Riverside, CA 92521, U.S.A.Department of Microbiology and Plant Pathology, University of California, Riverside, CA 92521, U.S.A.Sporangia of the potato late blight agent Phytophthora infestans are often used in studies of pathogen biology and plant responses to infection. Investigations of spore biology can be challenging in oomycetes because their sporangia are physiologically active and change in response to environmental factors and aging. Whether sporangia from artificial media and plant lesions are functionally equivalent has been a topic of debate. To address these issues, we compared the transcriptomes and infection ability of sporangia from rye-sucrose media, potato and tomato leaflets, and potato tubers. Small differences were observed between the mRNA profiles of sporangia from all sources, including variation in genes encoding metabolic enzymes, cell-wall-degrading enzymes, and ABC transporters. Small differences in sporangia age also resulted in variation in the transcriptome. Taking care to use sporangia of similar maturity, we observed that those sourced from media or plant lesions had similar rates of zoospore release and cyst germination. There were also no differences in infection rates or aggressiveness on leaflets, based on single-spore inoculation assays. Such results are discordant with those of a recent publication in this journal. Nevertheless, we conclude that sporangia from plant and media cultures are functionally similar and emphasize the importance of using “best practices” in experiments with sporangia to obtain reliable results.https://apsjournals.apsnet.org/doi/10.1094/MPMI-12-18-0349-TAoomycete–plant interactionsPhytophthora diseases |
| spellingShingle | Wiphawee Leesutthiphonchai Howard S. Judelson Phytophthora infestans Sporangia Produced in Artificial Media and Plant Lesions Have Subtly Divergent Transcription Profiles but Equivalent Infection Potential and Aggressiveness Molecular Plant-Microbe Interactions oomycete–plant interactions Phytophthora diseases |
| title | Phytophthora infestans Sporangia Produced in Artificial Media and Plant Lesions Have Subtly Divergent Transcription Profiles but Equivalent Infection Potential and Aggressiveness |
| title_full | Phytophthora infestans Sporangia Produced in Artificial Media and Plant Lesions Have Subtly Divergent Transcription Profiles but Equivalent Infection Potential and Aggressiveness |
| title_fullStr | Phytophthora infestans Sporangia Produced in Artificial Media and Plant Lesions Have Subtly Divergent Transcription Profiles but Equivalent Infection Potential and Aggressiveness |
| title_full_unstemmed | Phytophthora infestans Sporangia Produced in Artificial Media and Plant Lesions Have Subtly Divergent Transcription Profiles but Equivalent Infection Potential and Aggressiveness |
| title_short | Phytophthora infestans Sporangia Produced in Artificial Media and Plant Lesions Have Subtly Divergent Transcription Profiles but Equivalent Infection Potential and Aggressiveness |
| title_sort | phytophthora infestans sporangia produced in artificial media and plant lesions have subtly divergent transcription profiles but equivalent infection potential and aggressiveness |
| topic | oomycete–plant interactions Phytophthora diseases |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-12-18-0349-TA |
| work_keys_str_mv | AT wiphaweeleesutthiphonchai phytophthorainfestanssporangiaproducedinartificialmediaandplantlesionshavesubtlydivergenttranscriptionprofilesbutequivalentinfectionpotentialandaggressiveness AT howardsjudelson phytophthorainfestanssporangiaproducedinartificialmediaandplantlesionshavesubtlydivergenttranscriptionprofilesbutequivalentinfectionpotentialandaggressiveness |