Possibilities of electrochemical techniques in metallothionein and lead detection in fish tissues

In the present paper, we report on the use of adsorptive transfer stripping technique in connection with chronopotentiometric stripping analysis for metallothionein determination and of differential pulse anodic stripping voltammetry for lead detection in tissues of wild perch (Perca fluviatilis, n...

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Main Authors: S. Křížková, O. Zítka, V. Adam, M. Beklová, A. Horna, Z. Svobodová, B. Sures, L. Trnková, L. Zeman, R. Kizek
Format: Article
Language:English
Published: Czech Academy of Agricultural Sciences 2007-05-01
Series:Czech Journal of Animal Science
Subjects:
Online Access:https://cjas.agriculturejournals.cz/artkey/cjs-200705-0005_possibilities-of-electrochemical-techniques-in-metallothionein-and-lead-detection-in-fish-tissues.php
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author S. Křížková
O. Zítka
V. Adam
M. Beklová
A. Horna
Z. Svobodová
B. Sures
L. Trnková
L. Zeman
R. Kizek
author_facet S. Křížková
O. Zítka
V. Adam
M. Beklová
A. Horna
Z. Svobodová
B. Sures
L. Trnková
L. Zeman
R. Kizek
author_sort S. Křížková
collection DOAJ
description In the present paper, we report on the use of adsorptive transfer stripping technique in connection with chronopotentiometric stripping analysis for metallothionein determination and of differential pulse anodic stripping voltammetry for lead detection in tissues of wild perch (Perca fluviatilis, n = 6) from the Svratka River in Brno, Czech Republic. Primarily, we determined the content of MT in tissues (muscles, gonads, liver and spleen) of perch. We measured the highest content of MT in spleen and liver (100-350 ng MT per gram of fresh weight). We assume that the content of MT determined in perch tissues is probably related with the age of the fish and, therefore, with their exposition to heavy metals naturally occurring in the Svratka River. We detected a lead concentration in the tissues of one perch. It clearly follows from the results that the content of MT well correlates with the concentration of lead.
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spelling doaj.art-3ef028d1a0f14a8d80e2b122dffc0e7d2023-02-23T03:32:29ZengCzech Academy of Agricultural SciencesCzech Journal of Animal Science1212-18191805-93092007-05-0152514314810.17221/2232-CJAScjs-200705-0005Possibilities of electrochemical techniques in metallothionein and lead detection in fish tissuesS. Křížková0O. Zítka1V. Adam2M. Beklová3A. Horna4Z. Svobodová5B. Sures6L. Trnková7L. Zeman8R. Kizek9Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Brno, Czech RepublicDepartment of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Brno, Czech RepublicDepartment of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Brno, Czech RepublicDepartment of Veterinary Ecology and Environmental Protection, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech RepublicDepartment of Food Engineering and Chemistry, Faculty of Technology, Tomas Bata University, Zlin, Czech RepublicInstitute of Parasitology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech RepublicUniversität Duisburg-Essen, Angewande Zoologie/Hydrobiologie, Essen, GermanyDepartment of Chemistry, Faculty of Science, Masaryk University, Brno, Czech RepublicDepartment of Animal Nutrition and Forage Production, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Brno, Czech RepublicDepartment of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Brno, Czech RepublicIn the present paper, we report on the use of adsorptive transfer stripping technique in connection with chronopotentiometric stripping analysis for metallothionein determination and of differential pulse anodic stripping voltammetry for lead detection in tissues of wild perch (Perca fluviatilis, n = 6) from the Svratka River in Brno, Czech Republic. Primarily, we determined the content of MT in tissues (muscles, gonads, liver and spleen) of perch. We measured the highest content of MT in spleen and liver (100-350 ng MT per gram of fresh weight). We assume that the content of MT determined in perch tissues is probably related with the age of the fish and, therefore, with their exposition to heavy metals naturally occurring in the Svratka River. We detected a lead concentration in the tissues of one perch. It clearly follows from the results that the content of MT well correlates with the concentration of lead.https://cjas.agriculturejournals.cz/artkey/cjs-200705-0005_possibilities-of-electrochemical-techniques-in-metallothionein-and-lead-detection-in-fish-tissues.phpelectrochemical detectioncatalytic signalpeak hheavy metalsfishenvironmental pollution
spellingShingle S. Křížková
O. Zítka
V. Adam
M. Beklová
A. Horna
Z. Svobodová
B. Sures
L. Trnková
L. Zeman
R. Kizek
Possibilities of electrochemical techniques in metallothionein and lead detection in fish tissues
Czech Journal of Animal Science
electrochemical detection
catalytic signal
peak h
heavy metals
fish
environmental pollution
title Possibilities of electrochemical techniques in metallothionein and lead detection in fish tissues
title_full Possibilities of electrochemical techniques in metallothionein and lead detection in fish tissues
title_fullStr Possibilities of electrochemical techniques in metallothionein and lead detection in fish tissues
title_full_unstemmed Possibilities of electrochemical techniques in metallothionein and lead detection in fish tissues
title_short Possibilities of electrochemical techniques in metallothionein and lead detection in fish tissues
title_sort possibilities of electrochemical techniques in metallothionein and lead detection in fish tissues
topic electrochemical detection
catalytic signal
peak h
heavy metals
fish
environmental pollution
url https://cjas.agriculturejournals.cz/artkey/cjs-200705-0005_possibilities-of-electrochemical-techniques-in-metallothionein-and-lead-detection-in-fish-tissues.php
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