Physical Mapping of the <i>Anopheles</i> (<i>Nyssorhynchus</i>) <i>darlingi</i> Genomic Scaffolds
The genome assembly of <i>Anopheles darlingi</i> consists of 2221 scaffolds (N50 = 115,072 bp) and has a size spanning 136.94 Mbp. This assembly represents one of the smallest genomes among <i>Anopheles</i> species. <i>Anopheles darlingi</i> genomic DNA fragments...
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| Format: | Article |
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MDPI AG
2021-02-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/12/2/164 |
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| author | Míriam Silva Rafael Leticia Cegatti Bridi Igor V. Sharakhov Osvaldo Marinotti Maria V. Sharakhova Vladimir Timoshevskiy Giselle Moura Guimarães-Marques Valéria Silva Santos Carlos Gustavo Nunes da Silva Spartaco Astolfi-Filho Wanderli Pedro Tadei |
| author_facet | Míriam Silva Rafael Leticia Cegatti Bridi Igor V. Sharakhov Osvaldo Marinotti Maria V. Sharakhova Vladimir Timoshevskiy Giselle Moura Guimarães-Marques Valéria Silva Santos Carlos Gustavo Nunes da Silva Spartaco Astolfi-Filho Wanderli Pedro Tadei |
| author_sort | Míriam Silva Rafael |
| collection | DOAJ |
| description | The genome assembly of <i>Anopheles darlingi</i> consists of 2221 scaffolds (N50 = 115,072 bp) and has a size spanning 136.94 Mbp. This assembly represents one of the smallest genomes among <i>Anopheles</i> species. <i>Anopheles darlingi</i> genomic DNA fragments of ~37 Kb were cloned, end-sequenced, and used as probes for fluorescence in situ hybridization (FISH) with salivary gland polytene chromosomes. In total, we mapped nine DNA probes to scaffolds and autosomal arms. Comparative analysis of the <i>An. darlingi</i> scaffolds with homologous sequences of the <i>Anopheles albimanus</i> and <i>Anopheles gambiae</i> genomes identified chromosomal rearrangements among these species. Our results confirmed that physical mapping is a useful tool for anchoring genome assemblies to mosquito chromosomes. |
| first_indexed | 2024-03-09T00:52:20Z |
| format | Article |
| id | doaj.art-3ef1572a2f614520b6d9b4518a13960c |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-09T00:52:20Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-3ef1572a2f614520b6d9b4518a13960c2023-12-11T17:07:44ZengMDPI AGInsects2075-44502021-02-0112216410.3390/insects12020164Physical Mapping of the <i>Anopheles</i> (<i>Nyssorhynchus</i>) <i>darlingi</i> Genomic ScaffoldsMíriam Silva Rafael0Leticia Cegatti Bridi1Igor V. Sharakhov2Osvaldo Marinotti3Maria V. Sharakhova4Vladimir Timoshevskiy5Giselle Moura Guimarães-Marques6Valéria Silva Santos7Carlos Gustavo Nunes da Silva8Spartaco Astolfi-Filho9Wanderli Pedro Tadei10Coordenação de Sociedade Ambiente e Saúde, Laboratório de Vetores de Malária e Dengue, Instituto Nacional de Pesquisas da Amazônia, Av. André Araújo, 2936, Manaus, AM 69060-001, BrazilPrograma de Pós-Graduação em Genética, Conservação e Biologia Evolutiv, Instituto Nacional de Pesquisas da Amazônia, Manaus, AM 69060-001, BrazilDepartment of Entomology and Fralin Life Science Institute, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USAMTEKPrime, Aliso Viejo, CA 92656, USADepartment of Entomology and Fralin Life Science Institute, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USADepartment of Entomology and Fralin Life Science Institute, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USAPrograma de Pós-Graduação em Genética, Conservação e Biologia Evolutiv, Instituto Nacional de Pesquisas da Amazônia, Manaus, AM 69060-001, BrazilPrograma de Pós-Graduação em Genética, Conservação e Biologia Evolutiv, Instituto Nacional de Pesquisas da Amazônia, Manaus, AM 69060-001, BrazilPrograma de Pós-Graduação em Biotecnologia, Universidade Federal do Amazonas, Av. Rodrigo Otávio, 6.200. Coroado l, Manaus, AM 69080-900, BrazilLaboratorio de Tecnologias de DNA, Divisão de Biotecnologia, Centro de Apoio Multidisciplinar, Universi dade Federal do Amazonas, Av. Rodrigo Otávio, 6.200. Coroado l, Manaus, AM 69080-900, BrazilCoordenação de Sociedade Ambiente e Saúde, Laboratório de Vetores de Malária e Dengue, Instituto Nacional de Pesquisas da Amazônia, Av. André Araújo, 2936, Manaus, AM 69060-001, BrazilThe genome assembly of <i>Anopheles darlingi</i> consists of 2221 scaffolds (N50 = 115,072 bp) and has a size spanning 136.94 Mbp. This assembly represents one of the smallest genomes among <i>Anopheles</i> species. <i>Anopheles darlingi</i> genomic DNA fragments of ~37 Kb were cloned, end-sequenced, and used as probes for fluorescence in situ hybridization (FISH) with salivary gland polytene chromosomes. In total, we mapped nine DNA probes to scaffolds and autosomal arms. Comparative analysis of the <i>An. darlingi</i> scaffolds with homologous sequences of the <i>Anopheles albimanus</i> and <i>Anopheles gambiae</i> genomes identified chromosomal rearrangements among these species. Our results confirmed that physical mapping is a useful tool for anchoring genome assemblies to mosquito chromosomes.https://www.mdpi.com/2075-4450/12/2/164in situ hybridizationgenomics and cytogeneticssyntenypolytene chromosome |
| spellingShingle | Míriam Silva Rafael Leticia Cegatti Bridi Igor V. Sharakhov Osvaldo Marinotti Maria V. Sharakhova Vladimir Timoshevskiy Giselle Moura Guimarães-Marques Valéria Silva Santos Carlos Gustavo Nunes da Silva Spartaco Astolfi-Filho Wanderli Pedro Tadei Physical Mapping of the <i>Anopheles</i> (<i>Nyssorhynchus</i>) <i>darlingi</i> Genomic Scaffolds Insects in situ hybridization genomics and cytogenetics synteny polytene chromosome |
| title | Physical Mapping of the <i>Anopheles</i> (<i>Nyssorhynchus</i>) <i>darlingi</i> Genomic Scaffolds |
| title_full | Physical Mapping of the <i>Anopheles</i> (<i>Nyssorhynchus</i>) <i>darlingi</i> Genomic Scaffolds |
| title_fullStr | Physical Mapping of the <i>Anopheles</i> (<i>Nyssorhynchus</i>) <i>darlingi</i> Genomic Scaffolds |
| title_full_unstemmed | Physical Mapping of the <i>Anopheles</i> (<i>Nyssorhynchus</i>) <i>darlingi</i> Genomic Scaffolds |
| title_short | Physical Mapping of the <i>Anopheles</i> (<i>Nyssorhynchus</i>) <i>darlingi</i> Genomic Scaffolds |
| title_sort | physical mapping of the i anopheles i i nyssorhynchus i i darlingi i genomic scaffolds |
| topic | in situ hybridization genomics and cytogenetics synteny polytene chromosome |
| url | https://www.mdpi.com/2075-4450/12/2/164 |
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