Functional characterization of mammalian Wntless homolog in mammalian system
Wntless (GPR177) protein is a newly identified regulator of Wnt signals in Drosophila, but its cellular function in mammals is still unclear. In this study, we explored the expression pattern and potential cellular function of Wntless in mammalian cells. Wntless mRNA was expressed in many mouse tiss...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2012-07-01
|
| Series: | Kaohsiung Journal of Medical Sciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1607551X12000319 |
| _version_ | 1811214324439449600 |
|---|---|
| author | Li-Ting Wang Shih-Jong Wang Shih-Hsien Hsu |
| author_facet | Li-Ting Wang Shih-Jong Wang Shih-Hsien Hsu |
| author_sort | Li-Ting Wang |
| collection | DOAJ |
| description | Wntless (GPR177) protein is a newly identified regulator of Wnt signals in Drosophila, but its cellular function in mammals is still unclear. In this study, we explored the expression pattern and potential cellular function of Wntless in mammalian cells. Wntless mRNA was expressed in many mouse tissues, including the spleen, lung, kidney, thymus, and stomach, and lower levels of expression were detected in the mouse brain and testis. Expression of Wntless protein analyzed by Western blot and immunohistochemical staining was only detected in the submucosa, muscle, ganglia, and nerve cells of murine large intestines. Both immunofluorescence staining and subcellular fraction extraction analysis revealed that endogenous Wntless protein was expressed predominantly in the cytoplasmic organelles with a morphologically dot-shaped distribution. Furthermore, overexpression of Wntless could be corrected by and may activate the nuclear factor-κB (NF-κB) signaling pathway in cancer (HeLa) cells. These results suggest that Wntless plays a role in signaling regulation during the formation of cancer in addition to its role as a retromer protein in mammalian systems. |
| first_indexed | 2024-04-12T06:01:24Z |
| format | Article |
| id | doaj.art-3ef69f7200624342bda6b3d5f9936dc7 |
| institution | Directory Open Access Journal |
| issn | 1607-551X |
| language | English |
| last_indexed | 2024-04-12T06:01:24Z |
| publishDate | 2012-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Kaohsiung Journal of Medical Sciences |
| spelling | doaj.art-3ef69f7200624342bda6b3d5f9936dc72022-12-22T03:45:01ZengWileyKaohsiung Journal of Medical Sciences1607-551X2012-07-0128735536110.1016/j.kjms.2012.02.001Functional characterization of mammalian Wntless homolog in mammalian systemLi-Ting Wang0Shih-Jong Wang1Shih-Hsien Hsu2Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, TaiwanDivision of Hematology-Oncology, Department of Pediatrics, Changhua Christian Hospital, Changhua, TaiwanGraduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, TaiwanWntless (GPR177) protein is a newly identified regulator of Wnt signals in Drosophila, but its cellular function in mammals is still unclear. In this study, we explored the expression pattern and potential cellular function of Wntless in mammalian cells. Wntless mRNA was expressed in many mouse tissues, including the spleen, lung, kidney, thymus, and stomach, and lower levels of expression were detected in the mouse brain and testis. Expression of Wntless protein analyzed by Western blot and immunohistochemical staining was only detected in the submucosa, muscle, ganglia, and nerve cells of murine large intestines. Both immunofluorescence staining and subcellular fraction extraction analysis revealed that endogenous Wntless protein was expressed predominantly in the cytoplasmic organelles with a morphologically dot-shaped distribution. Furthermore, overexpression of Wntless could be corrected by and may activate the nuclear factor-κB (NF-κB) signaling pathway in cancer (HeLa) cells. These results suggest that Wntless plays a role in signaling regulation during the formation of cancer in addition to its role as a retromer protein in mammalian systems.http://www.sciencedirect.com/science/article/pii/S1607551X12000319GPR177NF-κBWntless |
| spellingShingle | Li-Ting Wang Shih-Jong Wang Shih-Hsien Hsu Functional characterization of mammalian Wntless homolog in mammalian system Kaohsiung Journal of Medical Sciences GPR177 NF-κB Wntless |
| title | Functional characterization of mammalian Wntless homolog in mammalian system |
| title_full | Functional characterization of mammalian Wntless homolog in mammalian system |
| title_fullStr | Functional characterization of mammalian Wntless homolog in mammalian system |
| title_full_unstemmed | Functional characterization of mammalian Wntless homolog in mammalian system |
| title_short | Functional characterization of mammalian Wntless homolog in mammalian system |
| title_sort | functional characterization of mammalian wntless homolog in mammalian system |
| topic | GPR177 NF-κB Wntless |
| url | http://www.sciencedirect.com/science/article/pii/S1607551X12000319 |
| work_keys_str_mv | AT litingwang functionalcharacterizationofmammalianwntlesshomologinmammaliansystem AT shihjongwang functionalcharacterizationofmammalianwntlesshomologinmammaliansystem AT shihhsienhsu functionalcharacterizationofmammalianwntlesshomologinmammaliansystem |