SDS-PAGE-Based Quantitative Assay for Screening of Kidney Stone Disease
<p>Abstract</p> <p>Kidney stone disease is a common health problem in industrialised nations. We developed a SDS-PAGE-based method to quantify Tamm Horsfall glycoprotein (THP) for screening of kidney stone disease. Urinary proteins were extracted by using ammonium sulphate precipit...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2009-01-01
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| Series: | Biological Procedures Online |
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| Online Access: | http://www.biologicalproceduresonline.com/content/11/1/145 |
| _version_ | 1818787643991785472 |
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| author | Wing-Seng Leong Wai-Hoe Lau Ismail Zhari Lay-Harn Gam |
| author_facet | Wing-Seng Leong Wai-Hoe Lau Ismail Zhari Lay-Harn Gam |
| author_sort | Wing-Seng Leong |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Kidney stone disease is a common health problem in industrialised nations. We developed a SDS-PAGE-based method to quantify Tamm Horsfall glycoprotein (THP) for screening of kidney stone disease. Urinary proteins were extracted by using ammonium sulphate precipitation at 0.27 g salt/mL urine. The resulted pellet was dissolved in TSE buffer. Ten microliters of the urinary proteins extract was loaded and separated on 10% SDS-PAGE under reducing condition. THP migrated as single band in SDS-PAGE. The assay reproducibility and repeatability were 4.8% CV and 2.6% CV, respectively. A total of 117 healthy subjects and 58 stone patients were tested using this assay, and a distinct cut-off (<it>P</it> < 0.05) at 5.6 μg/mL THP concentration was used to distinguish stone patients from healthy subjects. The sensitivity and specificity of the method were 92.3% and 83.3%, respectively.</p> |
| first_indexed | 2024-12-18T14:11:03Z |
| format | Article |
| id | doaj.art-3ef6cf0e697b43a1a40e9570a7fec78d |
| institution | Directory Open Access Journal |
| issn | 1480-9222 |
| language | English |
| last_indexed | 2024-12-18T14:11:03Z |
| publishDate | 2009-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Biological Procedures Online |
| spelling | doaj.art-3ef6cf0e697b43a1a40e9570a7fec78d2022-12-21T21:05:08ZengBMCBiological Procedures Online1480-92222009-01-01111145160SDS-PAGE-Based Quantitative Assay for Screening of Kidney Stone DiseaseWing-Seng LeongWai-Hoe LauIsmail ZhariLay-Harn Gam<p>Abstract</p> <p>Kidney stone disease is a common health problem in industrialised nations. We developed a SDS-PAGE-based method to quantify Tamm Horsfall glycoprotein (THP) for screening of kidney stone disease. Urinary proteins were extracted by using ammonium sulphate precipitation at 0.27 g salt/mL urine. The resulted pellet was dissolved in TSE buffer. Ten microliters of the urinary proteins extract was loaded and separated on 10% SDS-PAGE under reducing condition. THP migrated as single band in SDS-PAGE. The assay reproducibility and repeatability were 4.8% CV and 2.6% CV, respectively. A total of 117 healthy subjects and 58 stone patients were tested using this assay, and a distinct cut-off (<it>P</it> < 0.05) at 5.6 μg/mL THP concentration was used to distinguish stone patients from healthy subjects. The sensitivity and specificity of the method were 92.3% and 83.3%, respectively.</p>http://www.biologicalproceduresonline.com/content/11/1/145Kidney stone diseaseSDS-PAGETamm Horsfall Protein and diagnostic device |
| spellingShingle | Wing-Seng Leong Wai-Hoe Lau Ismail Zhari Lay-Harn Gam SDS-PAGE-Based Quantitative Assay for Screening of Kidney Stone Disease Biological Procedures Online Kidney stone disease SDS-PAGE Tamm Horsfall Protein and diagnostic device |
| title | SDS-PAGE-Based Quantitative Assay for Screening of Kidney Stone Disease |
| title_full | SDS-PAGE-Based Quantitative Assay for Screening of Kidney Stone Disease |
| title_fullStr | SDS-PAGE-Based Quantitative Assay for Screening of Kidney Stone Disease |
| title_full_unstemmed | SDS-PAGE-Based Quantitative Assay for Screening of Kidney Stone Disease |
| title_short | SDS-PAGE-Based Quantitative Assay for Screening of Kidney Stone Disease |
| title_sort | sds page based quantitative assay for screening of kidney stone disease |
| topic | Kidney stone disease SDS-PAGE Tamm Horsfall Protein and diagnostic device |
| url | http://www.biologicalproceduresonline.com/content/11/1/145 |
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