Diagnosis and Characterization of Leishmania Species in Giemsa-Stained slides by PCR-RFLP
Background: Direct identification of Leishmania species in Giemsa-stained slides without parasite culturing in the areas where multiple species exist, is very helpful. This study was designed to isolate Leishmani spp. from Giemsa-stained smears and to characterize them by PCR technique.Methods: A to...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2008-05-01
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| Series: | Iranian Journal of Public Health |
| Subjects: | |
| Online Access: | http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/6086.pdf&manuscript_id=6086 |
| _version_ | 1818862464020774912 |
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| author | E Kazemi-Rad M Mohebali H Hajjaran S Rezaei S Mamishi |
| author_facet | E Kazemi-Rad M Mohebali H Hajjaran S Rezaei S Mamishi |
| author_sort | E Kazemi-Rad |
| collection | DOAJ |
| description | Background: Direct identification of Leishmania species in Giemsa-stained slides without parasite culturing in the areas where multiple species exist, is very helpful. This study was designed to isolate Leishmani spp. from Giemsa-stained smears and to characterize them by PCR technique.Methods: A total of 48 Giemsa-Stained slides from confirmed cases of leishmaniasis were examined under a light micro­scope at×1000 and classified based on grading of Leishmania parasites. DNA from each slide was extracted separately and sub­jected to PCR. The ribosomal internal transcribed spacer 1 (ITS1) was amplified with specific primers and the PCR prod­ucts were digested with a restriction enzyme (HaeIII).Results: Of the 48 microscopy-positive slides, 43(89.6%) were positive by PCR-RFLP and Leishmania species were identi­fied. A statistically significant difference was observed between the both methods (P< 0.05) and also a concordance was found between microscopy and PCR-RFLP (k= 0.55).Conclusion: PCR-RFLP seems to be an effective method to identify Leishmania species from Giemsa-stained smears which have been collected from both infected humans and animal reservoir hosts in Iran. |
| first_indexed | 2024-12-19T10:00:16Z |
| format | Article |
| id | doaj.art-3ef7552b4b914436a5215661141f0da7 |
| institution | Directory Open Access Journal |
| issn | 2251-6085 |
| language | English |
| last_indexed | 2024-12-19T10:00:16Z |
| publishDate | 2008-05-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Public Health |
| spelling | doaj.art-3ef7552b4b914436a5215661141f0da72022-12-21T20:26:40ZengTehran University of Medical SciencesIranian Journal of Public Health2251-60852008-05-013715460Diagnosis and Characterization of Leishmania Species in Giemsa-Stained slides by PCR-RFLPE Kazemi-RadM MohebaliH HajjaranS RezaeiS MamishiBackground: Direct identification of Leishmania species in Giemsa-stained slides without parasite culturing in the areas where multiple species exist, is very helpful. This study was designed to isolate Leishmani spp. from Giemsa-stained smears and to characterize them by PCR technique.Methods: A total of 48 Giemsa-Stained slides from confirmed cases of leishmaniasis were examined under a light micro­scope at×1000 and classified based on grading of Leishmania parasites. DNA from each slide was extracted separately and sub­jected to PCR. The ribosomal internal transcribed spacer 1 (ITS1) was amplified with specific primers and the PCR prod­ucts were digested with a restriction enzyme (HaeIII).Results: Of the 48 microscopy-positive slides, 43(89.6%) were positive by PCR-RFLP and Leishmania species were identi­fied. A statistically significant difference was observed between the both methods (P< 0.05) and also a concordance was found between microscopy and PCR-RFLP (k= 0.55).Conclusion: PCR-RFLP seems to be an effective method to identify Leishmania species from Giemsa-stained smears which have been collected from both infected humans and animal reservoir hosts in Iran.http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/6086.pdf&manuscript_id=6086Leishmania speciesGiemsa-stained smear |
| spellingShingle | E Kazemi-Rad M Mohebali H Hajjaran S Rezaei S Mamishi Diagnosis and Characterization of Leishmania Species in Giemsa-Stained slides by PCR-RFLP Iranian Journal of Public Health Leishmania species Giemsa-stained smear |
| title | Diagnosis and Characterization of Leishmania Species in Giemsa-Stained slides by PCR-RFLP |
| title_full | Diagnosis and Characterization of Leishmania Species in Giemsa-Stained slides by PCR-RFLP |
| title_fullStr | Diagnosis and Characterization of Leishmania Species in Giemsa-Stained slides by PCR-RFLP |
| title_full_unstemmed | Diagnosis and Characterization of Leishmania Species in Giemsa-Stained slides by PCR-RFLP |
| title_short | Diagnosis and Characterization of Leishmania Species in Giemsa-Stained slides by PCR-RFLP |
| title_sort | diagnosis and characterization of leishmania species in giemsa stained slides by pcr rflp |
| topic | Leishmania species Giemsa-stained smear |
| url | http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/6086.pdf&manuscript_id=6086 |
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