A preliminary study of cross-amplified microsatellite loci using molted feathers from a near-threatened Painted Stork (Mycteria leucocephala) population of north India as a DNA source
Abstract Objective In continuation of an earlier study in which we reported the cross-amplification of Wood stork microsatellites on the DNA obtained from molted feathers of Painted stork (Mycteria leucocephala), here we investigated the nature of cross-amplified microsatellites and the effect of no...
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| Format: | Article |
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BMC
2017-11-01
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| Series: | BMC Research Notes |
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| Online Access: | http://link.springer.com/article/10.1186/s13104-017-2932-y |
| _version_ | 1818539217976819712 |
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| author | Bharat Bhushan Sharma Basu Dev Banerjee Abdul Jamil Urfi |
| author_facet | Bharat Bhushan Sharma Basu Dev Banerjee Abdul Jamil Urfi |
| author_sort | Bharat Bhushan Sharma |
| collection | DOAJ |
| description | Abstract Objective In continuation of an earlier study in which we reported the cross-amplification of Wood stork microsatellites on the DNA obtained from molted feathers of Painted stork (Mycteria leucocephala), here we investigated the nature of cross-amplified microsatellites and the effect of non-invasive samples on cross-amplification success. In a limited manner, we also addressed the genetic diversity and differentiation in a north Indian population of the Painted Stork examined over three nesting seasons. Results Among the nine cross-amplified loci, only 5 were polymorphic. Three and 6 loci exhibited low (< 50%) and high amplification success rates (> 80), respectively. For 36 of 145 samples most of the loci failed to amplify. For genetic diversity, only 3 loci could be used since others exhibited low amplification and linkage disequilibrium. Probability of identity (0.034) was not low enough to develop a confidence that the similar genotypes originate from the same individual. Forty-two unique genotypes were identified. In 3 loci, a low to moderate level of genetic diversity (mean He = 0.435) was reported. Non-significant Fst (0.003, P = 0.230), G’stH (0.005, P = 0.247) and Dest (0.003, P = 0.250) values indicate a lack of structuring in temporally distributed populations of Delhi Zoo. The limitations and uniqueness of this study are discussed. |
| first_indexed | 2024-12-11T21:39:08Z |
| format | Article |
| id | doaj.art-3efca30d807e4cc38636ece72e29dcca |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-12-11T21:39:08Z |
| publishDate | 2017-11-01 |
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| spelling | doaj.art-3efca30d807e4cc38636ece72e29dcca2022-12-22T00:49:54ZengBMCBMC Research Notes1756-05002017-11-011011610.1186/s13104-017-2932-yA preliminary study of cross-amplified microsatellite loci using molted feathers from a near-threatened Painted Stork (Mycteria leucocephala) population of north India as a DNA sourceBharat Bhushan Sharma0Basu Dev Banerjee1Abdul Jamil Urfi2Department of Environmental Studies, University of DelhiEnvironmental Biochemistry & Molecular Biology Laboratory, Department of Biochemistry, University College of Medical Sciences & Guru Teg Bahadur Hospital, University of DelhiDepartment of Environmental Studies, University of DelhiAbstract Objective In continuation of an earlier study in which we reported the cross-amplification of Wood stork microsatellites on the DNA obtained from molted feathers of Painted stork (Mycteria leucocephala), here we investigated the nature of cross-amplified microsatellites and the effect of non-invasive samples on cross-amplification success. In a limited manner, we also addressed the genetic diversity and differentiation in a north Indian population of the Painted Stork examined over three nesting seasons. Results Among the nine cross-amplified loci, only 5 were polymorphic. Three and 6 loci exhibited low (< 50%) and high amplification success rates (> 80), respectively. For 36 of 145 samples most of the loci failed to amplify. For genetic diversity, only 3 loci could be used since others exhibited low amplification and linkage disequilibrium. Probability of identity (0.034) was not low enough to develop a confidence that the similar genotypes originate from the same individual. Forty-two unique genotypes were identified. In 3 loci, a low to moderate level of genetic diversity (mean He = 0.435) was reported. Non-significant Fst (0.003, P = 0.230), G’stH (0.005, P = 0.247) and Dest (0.003, P = 0.250) values indicate a lack of structuring in temporally distributed populations of Delhi Zoo. The limitations and uniqueness of this study are discussed.http://link.springer.com/article/10.1186/s13104-017-2932-yPainted StorkMolted feathersMicrosatellitesWood StorkCross-amplificationGenetic diversity |
| spellingShingle | Bharat Bhushan Sharma Basu Dev Banerjee Abdul Jamil Urfi A preliminary study of cross-amplified microsatellite loci using molted feathers from a near-threatened Painted Stork (Mycteria leucocephala) population of north India as a DNA source BMC Research Notes Painted Stork Molted feathers Microsatellites Wood Stork Cross-amplification Genetic diversity |
| title | A preliminary study of cross-amplified microsatellite loci using molted feathers from a near-threatened Painted Stork (Mycteria leucocephala) population of north India as a DNA source |
| title_full | A preliminary study of cross-amplified microsatellite loci using molted feathers from a near-threatened Painted Stork (Mycteria leucocephala) population of north India as a DNA source |
| title_fullStr | A preliminary study of cross-amplified microsatellite loci using molted feathers from a near-threatened Painted Stork (Mycteria leucocephala) population of north India as a DNA source |
| title_full_unstemmed | A preliminary study of cross-amplified microsatellite loci using molted feathers from a near-threatened Painted Stork (Mycteria leucocephala) population of north India as a DNA source |
| title_short | A preliminary study of cross-amplified microsatellite loci using molted feathers from a near-threatened Painted Stork (Mycteria leucocephala) population of north India as a DNA source |
| title_sort | preliminary study of cross amplified microsatellite loci using molted feathers from a near threatened painted stork mycteria leucocephala population of north india as a dna source |
| topic | Painted Stork Molted feathers Microsatellites Wood Stork Cross-amplification Genetic diversity |
| url | http://link.springer.com/article/10.1186/s13104-017-2932-y |
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