Identification of Species Causing Cutaneous Leishmaniasis by PCR in Chahbahar, Iran
ABSTRACT Background and Objective: Chabahar is in Southern Iran located near the Iran-Pakistan border. Since leishmaniasis is an emerging disease in this region, this study aimed to diagnose the disease and identify different species of Leishmania parasite in the patients referred to the cen...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Golestan University of Medical Sciences
2016-03-01
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| Series: | Medical Laboratory Journal |
| Subjects: | |
| Online Access: | http://mlj.goums.ac.ir/article-1-853-en.html |
| _version_ | 1819078005166702592 |
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| author | Hosein Soleimanpoor Mansour Dabirzadeh Bahman Fooladi |
| author_facet | Hosein Soleimanpoor Mansour Dabirzadeh Bahman Fooladi |
| author_sort | Hosein Soleimanpoor |
| collection | DOAJ |
| description | ABSTRACT
Background and Objective: Chabahar is in Southern Iran located near the Iran-Pakistan border. Since leishmaniasis is an emerging disease in this region, this study aimed to diagnose the disease and identify different species of Leishmania parasite in the patients referred to the central laboratory.
Methods: This descriptive cross-sectional study was conducted in 2011-2012 on patients referred to the central laboratory in the city of Chabahar. The sampling of lesions, slide preparation, culture and PCR specific for kinetoplast DNA (kDNA), extracted from the media and slides, were performed. The data collected by a questionnaire were analyzed by the SPSS software.
Results: The resulted bands from the 48 tested cutaneous leishmaniasis isolates were compared with the standard strains of Leishmania tropica, L. infantum and L. major. All 48 investigated bands were in the 620bp region, which is related to L. major.
Conclusion: Since PCR has high sensitivity and specificity, it is recommended to use kDNA (present in a unique organelle called kinetoplast) for the routine diagnosis and treatment of the disease. |
| first_indexed | 2024-12-21T19:06:13Z |
| format | Article |
| id | doaj.art-3efce5aabfe94de3869faef1520cb95a |
| institution | Directory Open Access Journal |
| issn | 2538-4449 |
| language | English |
| last_indexed | 2024-12-21T19:06:13Z |
| publishDate | 2016-03-01 |
| publisher | Golestan University of Medical Sciences |
| record_format | Article |
| series | Medical Laboratory Journal |
| spelling | doaj.art-3efce5aabfe94de3869faef1520cb95a2022-12-21T18:53:20ZengGolestan University of Medical SciencesMedical Laboratory Journal2538-44492016-03-011024651Identification of Species Causing Cutaneous Leishmaniasis by PCR in Chahbahar, IranHosein Soleimanpoor0Mansour Dabirzadeh1Bahman Fooladi2 Zabol University of Medical Sciences, Zabol, Iran Faculty of Medicine, Zabol University of Medical Sciences, Zabol, Iran Zabol University of Medical Sciences, Zabol, Iran ABSTRACT Background and Objective: Chabahar is in Southern Iran located near the Iran-Pakistan border. Since leishmaniasis is an emerging disease in this region, this study aimed to diagnose the disease and identify different species of Leishmania parasite in the patients referred to the central laboratory. Methods: This descriptive cross-sectional study was conducted in 2011-2012 on patients referred to the central laboratory in the city of Chabahar. The sampling of lesions, slide preparation, culture and PCR specific for kinetoplast DNA (kDNA), extracted from the media and slides, were performed. The data collected by a questionnaire were analyzed by the SPSS software. Results: The resulted bands from the 48 tested cutaneous leishmaniasis isolates were compared with the standard strains of Leishmania tropica, L. infantum and L. major. All 48 investigated bands were in the 620bp region, which is related to L. major. Conclusion: Since PCR has high sensitivity and specificity, it is recommended to use kDNA (present in a unique organelle called kinetoplast) for the routine diagnosis and treatment of the disease.http://mlj.goums.ac.ir/article-1-853-en.htmlleishmaniasiscutaneouspolymerase chain reactioniran. |
| spellingShingle | Hosein Soleimanpoor Mansour Dabirzadeh Bahman Fooladi Identification of Species Causing Cutaneous Leishmaniasis by PCR in Chahbahar, Iran Medical Laboratory Journal leishmaniasis cutaneous polymerase chain reaction iran. |
| title | Identification of Species Causing Cutaneous Leishmaniasis by PCR in Chahbahar, Iran |
| title_full | Identification of Species Causing Cutaneous Leishmaniasis by PCR in Chahbahar, Iran |
| title_fullStr | Identification of Species Causing Cutaneous Leishmaniasis by PCR in Chahbahar, Iran |
| title_full_unstemmed | Identification of Species Causing Cutaneous Leishmaniasis by PCR in Chahbahar, Iran |
| title_short | Identification of Species Causing Cutaneous Leishmaniasis by PCR in Chahbahar, Iran |
| title_sort | identification of species causing cutaneous leishmaniasis by pcr in chahbahar iran |
| topic | leishmaniasis cutaneous polymerase chain reaction iran. |
| url | http://mlj.goums.ac.ir/article-1-853-en.html |
| work_keys_str_mv | AT hoseinsoleimanpoor identificationofspeciescausingcutaneousleishmaniasisbypcrinchahbahariran AT mansourdabirzadeh identificationofspeciescausingcutaneousleishmaniasisbypcrinchahbahariran AT bahmanfooladi identificationofspeciescausingcutaneousleishmaniasisbypcrinchahbahariran |