Antioxidant Activity of Bioactive Peptide Fractions from Germinated Soybeans Conjugated to Fe<sub>3</sub>O<sub>4</sub> Nanoparticles by the Ugi Multicomponent Reaction
The conjugation of biomolecules to magnetic nanoparticles has emerged as promising approach in biomedicine as the treatment of several diseases, such as cancer. In this study, conjugation of bioactive peptide fractions from germinated soybeans to magnetite nanoparticles was achieved. Different fract...
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| Format: | Article |
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MDPI AG
2021-09-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/19/5726 |
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| author | Yarelys Elena Augusto-Jimenez Marcela González-Montoya Dany Naranjo-Feliciano Daniel Uribe-Ramírez Eliseo Cristiani-Urbina Carlos Díaz-Águila Hernani Yee-Madeira Rosalva Mora-Escobedo |
| author_facet | Yarelys Elena Augusto-Jimenez Marcela González-Montoya Dany Naranjo-Feliciano Daniel Uribe-Ramírez Eliseo Cristiani-Urbina Carlos Díaz-Águila Hernani Yee-Madeira Rosalva Mora-Escobedo |
| author_sort | Yarelys Elena Augusto-Jimenez |
| collection | DOAJ |
| description | The conjugation of biomolecules to magnetic nanoparticles has emerged as promising approach in biomedicine as the treatment of several diseases, such as cancer. In this study, conjugation of bioactive peptide fractions from germinated soybeans to magnetite nanoparticles was achieved. Different fractions of germinated soybean peptides (>10 kDa and 5–10 kDa) were for the first time conjugated to previously coated magnetite nanoparticles (with 3-aminopropyltriethoxysilane (APTES) and sodium citrate) by the Ugi four-component reaction. The crystallinity of the nanoparticles was corroborated by X-ray diffraction, while the particle size was determined by scanning transmission electron microscopy. The analyses were carried out using infrared and ultraviolet–visible spectroscopy, dynamic light scattering, and thermogravimetry, which confirmed the coating and functionalization of the magnetite nanoparticles and conjugation of different peptide fractions on their surfaces. The antioxidant activity of the conjugates was determined by the reducing power and hydroxyl radical scavenging activity. The nanoparticles synthesized represent promising materials, as they have found applications in bionanotechnology for enhanced treatment of diseases, such as cancer, due to a higher antioxidant capacity than that of fractions without conjugation. The highest antioxidant capacity was observed for a >10 kDa peptide fraction conjugated to the magnetite nanoparticles coated with APTES. |
| first_indexed | 2024-03-10T06:54:50Z |
| format | Article |
| id | doaj.art-3efdd57f7b914b9b9c1e79c73bdcc66a |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T06:54:50Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-3efdd57f7b914b9b9c1e79c73bdcc66a2023-11-22T16:31:43ZengMDPI AGMolecules1420-30492021-09-012619572610.3390/molecules26195726Antioxidant Activity of Bioactive Peptide Fractions from Germinated Soybeans Conjugated to Fe<sub>3</sub>O<sub>4</sub> Nanoparticles by the Ugi Multicomponent ReactionYarelys Elena Augusto-Jimenez0Marcela González-Montoya1Dany Naranjo-Feliciano2Daniel Uribe-Ramírez3Eliseo Cristiani-Urbina4Carlos Díaz-Águila5Hernani Yee-Madeira6Rosalva Mora-Escobedo7Instituto Politécnico Nacional–ESFM, U.P.A.L.M., San Pedro Zacatenco, Ciudad de Mexico 07738, MexicoInstituto Politécnico Nacional–ENCB, U.P.A.L.M., San Pedro Zacatenco, Ciudad de Mexico 07738, MexicoCentro Nacional de Sanidad Agropecuaria, San Jose de las Lajas 32700, Mayabeque, CubaInstituto Politécnico Nacional–ENCB, U.P.A.L.M., San Pedro Zacatenco, Ciudad de Mexico 07738, MexicoInstituto Politécnico Nacional–ENCB, U.P.A.L.M., San Pedro Zacatenco, Ciudad de Mexico 07738, MexicoCentro de Biomateriales, Universidad de La Habana, Plaza de la Revolucion 10400, La Habana, CubaInstituto Politécnico Nacional–ESFM, U.P.A.L.M., San Pedro Zacatenco, Ciudad de Mexico 07738, MexicoInstituto Politécnico Nacional–ENCB, U.P.A.L.M., San Pedro Zacatenco, Ciudad de Mexico 07738, MexicoThe conjugation of biomolecules to magnetic nanoparticles has emerged as promising approach in biomedicine as the treatment of several diseases, such as cancer. In this study, conjugation of bioactive peptide fractions from germinated soybeans to magnetite nanoparticles was achieved. Different fractions of germinated soybean peptides (>10 kDa and 5–10 kDa) were for the first time conjugated to previously coated magnetite nanoparticles (with 3-aminopropyltriethoxysilane (APTES) and sodium citrate) by the Ugi four-component reaction. The crystallinity of the nanoparticles was corroborated by X-ray diffraction, while the particle size was determined by scanning transmission electron microscopy. The analyses were carried out using infrared and ultraviolet–visible spectroscopy, dynamic light scattering, and thermogravimetry, which confirmed the coating and functionalization of the magnetite nanoparticles and conjugation of different peptide fractions on their surfaces. The antioxidant activity of the conjugates was determined by the reducing power and hydroxyl radical scavenging activity. The nanoparticles synthesized represent promising materials, as they have found applications in bionanotechnology for enhanced treatment of diseases, such as cancer, due to a higher antioxidant capacity than that of fractions without conjugation. The highest antioxidant capacity was observed for a >10 kDa peptide fraction conjugated to the magnetite nanoparticles coated with APTES.https://www.mdpi.com/1420-3049/26/19/5726soybeanpeptidesantioxidant capacitymagnetite nanoparticlesconjugationmulticomponent reaction |
| spellingShingle | Yarelys Elena Augusto-Jimenez Marcela González-Montoya Dany Naranjo-Feliciano Daniel Uribe-Ramírez Eliseo Cristiani-Urbina Carlos Díaz-Águila Hernani Yee-Madeira Rosalva Mora-Escobedo Antioxidant Activity of Bioactive Peptide Fractions from Germinated Soybeans Conjugated to Fe<sub>3</sub>O<sub>4</sub> Nanoparticles by the Ugi Multicomponent Reaction Molecules soybean peptides antioxidant capacity magnetite nanoparticles conjugation multicomponent reaction |
| title | Antioxidant Activity of Bioactive Peptide Fractions from Germinated Soybeans Conjugated to Fe<sub>3</sub>O<sub>4</sub> Nanoparticles by the Ugi Multicomponent Reaction |
| title_full | Antioxidant Activity of Bioactive Peptide Fractions from Germinated Soybeans Conjugated to Fe<sub>3</sub>O<sub>4</sub> Nanoparticles by the Ugi Multicomponent Reaction |
| title_fullStr | Antioxidant Activity of Bioactive Peptide Fractions from Germinated Soybeans Conjugated to Fe<sub>3</sub>O<sub>4</sub> Nanoparticles by the Ugi Multicomponent Reaction |
| title_full_unstemmed | Antioxidant Activity of Bioactive Peptide Fractions from Germinated Soybeans Conjugated to Fe<sub>3</sub>O<sub>4</sub> Nanoparticles by the Ugi Multicomponent Reaction |
| title_short | Antioxidant Activity of Bioactive Peptide Fractions from Germinated Soybeans Conjugated to Fe<sub>3</sub>O<sub>4</sub> Nanoparticles by the Ugi Multicomponent Reaction |
| title_sort | antioxidant activity of bioactive peptide fractions from germinated soybeans conjugated to fe sub 3 sub o sub 4 sub nanoparticles by the ugi multicomponent reaction |
| topic | soybean peptides antioxidant capacity magnetite nanoparticles conjugation multicomponent reaction |
| url | https://www.mdpi.com/1420-3049/26/19/5726 |
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