CRISPR/Cas9 Targeted Editing of Genes Associated With Fungal Susceptibility in Vitis vinifera L. cv. Thompson Seedless Using Geminivirus-Derived Replicons

CRISPR/Cas9 Targeted Editing of Genes Associated With Fungal Susceptibility in Vitis vinifera L. cv. Thompson Seedless Using Geminivirus-Derived Replicons

The woody nature of grapevine (Vitis vinifera L.) has hindered the development of efficient gene editing strategies to improve this species. The lack of highly efficient gene transfer techniques, which, furthermore, are applied in multicellular explants such as somatic embryos, are additional techni...

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Bibliographic Details
Main Authors: Felipe Olivares, Rodrigo Loyola, Blanca Olmedo, María de los Ángeles Miccono, Carlos Aguirre, Ricardo Vergara, Danae Riquelme, Gabriela Madrid, Philippe Plantat, Roxana Mora, Daniel Espinoza, Humberto Prieto
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-12-01
Series:Frontiers in Plant Science
Subjects:
grapevine gene editing
BeYDV-derived vector
Agrobacterium-mediated transformation
paired gRNA gene editing
fungal susceptibility genes
Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2021.791030/full

Internet

https://www.frontiersin.org/articles/10.3389/fpls.2021.791030/full

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