Implementation of the asparaginase activity assessment technique for clinical use: experience of a Brazilian Center
Abstract Acute lymphoid leukemia is a childhood cancer that in high-income countries has event-free survival rates of 80% and global survival rates of 90%. In Brazil these rates are under 70%. This difference may be due to the implementation of supportive care, including the assessment of asparagina...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2020-12-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-020-78549-y |
| _version_ | 1818752470298394624 |
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| author | Daiane Keller Cecconello Ciliana Rechenmacher Isabel Werlang Priscila Pini Zenatti Jose Andres Yunes Ana Paula Alegretti Claudia Lanvers-Kaminsky Liane Esteves Daudt Mariana Bohns Michalowski |
| author_facet | Daiane Keller Cecconello Ciliana Rechenmacher Isabel Werlang Priscila Pini Zenatti Jose Andres Yunes Ana Paula Alegretti Claudia Lanvers-Kaminsky Liane Esteves Daudt Mariana Bohns Michalowski |
| author_sort | Daiane Keller Cecconello |
| collection | DOAJ |
| description | Abstract Acute lymphoid leukemia is a childhood cancer that in high-income countries has event-free survival rates of 80% and global survival rates of 90%. In Brazil these rates are under 70%. This difference may be due to the implementation of supportive care, including the assessment of asparaginase (ASNase) activity. ASNase may cause hypersensitivity reactions and silent drug inactivation. For this reason, ASNase activity monitoring is an essential tool to ensure an effective treatment. Our aim was to implement an ASNase activity measurement technique at a hospital setting. samples from children who were given Escherichia coli-derived ASNase were collected. The results of the analyses conducted in our laboratory Hospital de Clínicas de Porto Alegre were compared to those of two institutions: Centro Infantil Boldrini and University of Munster. 262 samples were assessed. The results of the first analyses were compared with those obtained at Centro Infantil Boldrini and showed an ICC of 0.954. Thirty samples were sent to the University of Munster and presented an ICC was 0.960. Our results, when compared to those of national and international centers, showed an excellent agreement. The study was able to implement an ASNase activity test to monitor the treatment. |
| first_indexed | 2024-12-18T04:51:58Z |
| format | Article |
| id | doaj.art-3f0393b11e36490cb2ac7fd60d863415 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-18T04:51:58Z |
| publishDate | 2020-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-3f0393b11e36490cb2ac7fd60d8634152022-12-21T21:20:23ZengNature PortfolioScientific Reports2045-23222020-12-011011610.1038/s41598-020-78549-yImplementation of the asparaginase activity assessment technique for clinical use: experience of a Brazilian CenterDaiane Keller Cecconello0Ciliana Rechenmacher1Isabel Werlang2Priscila Pini Zenatti3Jose Andres Yunes4Ana Paula Alegretti5Claudia Lanvers-Kaminsky6Liane Esteves Daudt7Mariana Bohns Michalowski8Graduate Program in Child and Adolescent Health, Universidade Federal do Rio Grande do SulGraduate Program in Child and Adolescent Health, Universidade Federal do Rio Grande do SulTranslational Pediatrics Laboratory, Experimental Research Center, Hospital de Clínicas de Porto AlegreCentro Infantil BoldriniCentro Infantil BoldriniHospital de Clínicas de Porto AlegreUniversity Children’s Hospital of MünsterGraduate Program in Child and Adolescent Health, Universidade Federal do Rio Grande do SulGraduate Program in Child and Adolescent Health, Universidade Federal do Rio Grande do SulAbstract Acute lymphoid leukemia is a childhood cancer that in high-income countries has event-free survival rates of 80% and global survival rates of 90%. In Brazil these rates are under 70%. This difference may be due to the implementation of supportive care, including the assessment of asparaginase (ASNase) activity. ASNase may cause hypersensitivity reactions and silent drug inactivation. For this reason, ASNase activity monitoring is an essential tool to ensure an effective treatment. Our aim was to implement an ASNase activity measurement technique at a hospital setting. samples from children who were given Escherichia coli-derived ASNase were collected. The results of the analyses conducted in our laboratory Hospital de Clínicas de Porto Alegre were compared to those of two institutions: Centro Infantil Boldrini and University of Munster. 262 samples were assessed. The results of the first analyses were compared with those obtained at Centro Infantil Boldrini and showed an ICC of 0.954. Thirty samples were sent to the University of Munster and presented an ICC was 0.960. Our results, when compared to those of national and international centers, showed an excellent agreement. The study was able to implement an ASNase activity test to monitor the treatment.https://doi.org/10.1038/s41598-020-78549-y |
| spellingShingle | Daiane Keller Cecconello Ciliana Rechenmacher Isabel Werlang Priscila Pini Zenatti Jose Andres Yunes Ana Paula Alegretti Claudia Lanvers-Kaminsky Liane Esteves Daudt Mariana Bohns Michalowski Implementation of the asparaginase activity assessment technique for clinical use: experience of a Brazilian Center Scientific Reports |
| title | Implementation of the asparaginase activity assessment technique for clinical use: experience of a Brazilian Center |
| title_full | Implementation of the asparaginase activity assessment technique for clinical use: experience of a Brazilian Center |
| title_fullStr | Implementation of the asparaginase activity assessment technique for clinical use: experience of a Brazilian Center |
| title_full_unstemmed | Implementation of the asparaginase activity assessment technique for clinical use: experience of a Brazilian Center |
| title_short | Implementation of the asparaginase activity assessment technique for clinical use: experience of a Brazilian Center |
| title_sort | implementation of the asparaginase activity assessment technique for clinical use experience of a brazilian center |
| url | https://doi.org/10.1038/s41598-020-78549-y |
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